Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,636,018 T→C 6.0% L129P (CTG→CCG)  PP_4948 → acyl‑CoA dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,636,0180TC 6.0% 192.9 / 2.7 66L129P (CTG→CCG) PP_4948acyl‑CoA dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base T (32/30);  new base C (2/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

CCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGACCGAACCCCACGCTGGCTCGTCGCTGGCCGACATACGCAC  >  NC_002947/5635871‑5636165
                                                                                                                                                   |                                                                                                                                                   
ccgccgTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCt                                                                                                                                                     >  6:195869/1‑148 (MQ=255)
 cgccgTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGa                                                                                                                                                   <  5:78700/149‑1 (MQ=255)
     gTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCAt                                                                                                                                               <  7:259439/149‑1 (MQ=255)
        gATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATggg                                                                                                                                            >  8:310576/1‑149 (MQ=255)
            ccTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGcgcg                                                                                                                                        <  8:251888/149‑1 (MQ=255)
             cTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCCCCACGGCCTACCCGTTCCTGACCATGGGCGCgg                                                                                                                                       >  7:352211/1‑149 (MQ=255)
                  tCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAAc                                                                                                                                  >  7:188872/1‑149 (MQ=255)
                  tCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAAc                                                                                                                                  >  2:30417/1‑149 (MQ=255)
                    gAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAAc                                                                                                                                  <  8:188872/147‑1 (MQ=255)
                      aGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGa                                                                                                                              >  2:177873/1‑149 (MQ=255)
                                  cAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCg                                                                                                                  >  5:31441/1‑149 (MQ=255)
                                   aaCGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCg                                                                                                                  <  8:109322/148‑1 (MQ=255)
                                      gCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATgg                                                                                                                                             >  8:83408/1‑118 (MQ=255)
                                      gCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATgg                                                                                                                                             <  7:83408/118‑1 (MQ=255)
                                       ccAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTcc                                                                                                             <  5:195869/149‑1 (MQ=255)
                                        cAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTcc                                                                                                             >  8:142704/1‑148 (MQ=255)
                                         aaCCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCga                                                                                                                          >  7:205317/1‑134 (MQ=255)
                                         aaCCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCga                                                                                                                          <  8:205317/134‑1 (MQ=255)
                                         aaCCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGa                                                                                                           <  7:310576/149‑1 (MQ=255)
                                         aaCCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGa                                                                                                           >  1:322221/1‑149 (MQ=255)
                                          aCCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGAt                                                                                                          <  7:142704/149‑1 (MQ=255)
                                          aCCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGAt                                                                                                          <  5:378164/149‑1 (MQ=255)
                                             gggACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGaa                                                                                                       <  1:177873/149‑1 (MQ=255)
                                             gggACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGaa                                                                                                       <  1:30417/149‑1 (MQ=255)
                                              ggACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAAc                                                                                                      <  5:86472/149‑1 (MQ=255)
                                              ggACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCCGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGaa                                                                                                       >  5:151304/1‑148 (MQ=255)
                                              ggACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCCGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGaa                                                                                                       >  5:156891/1‑148 (MQ=255)
                                                  ttCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGGGCGGCCAACCTGATCGAGAGCTTCGGCTcc                                                                                                             >  5:319027/1‑138 (MQ=255)
                                                  ttCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTcc                                                                                                             <  6:319027/138‑1 (MQ=255)
                                                  ttCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGAAAGCCTGCATCTCCAACTTCCAGGCCGCCAACGCCGGCACAACGGCCTACCCGTTACTGACCATGGGCGCGGCCAACCTGATAGAGCGCTTCGGCTCCGATTAACAGaa                                                                                                  >  6:214770/1‑149 (MQ=255)
                                                      aGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAgcgc                                                                                              >  6:255094/1‑149 (MQ=255)
                                                                 aTGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGc                                                                                    >  2:24820/1‑148 (MQ=255)
                                                                   gCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAAcc                                                                                                                                 <  1:297523/101‑1 (MQ=255)
                                                                   gCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAAcc                                                                                                                                 >  2:297523/1‑101 (MQ=255)
                                                                     atcttcccAGCCTGGTCTCGCAAGCCTGCATCGCCCAATTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAAACTGATCGAGAGCTTAGGCTCCGATGAACAGAAGAGCCTGTTCCAGCaaac                                                                                >  8:184314/6‑146 (MQ=255)
                                                                            cAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATc                                                                         >  1:219925/1‑148 (MQ=255)
                                                                            cAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATc                                                                         >  3:120821/1‑148 (MQ=255)
                                                                            cAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCg                                                                        >  2:23099/1‑149 (MQ=255)
                                                                                   gTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGcc                                                                  <  2:322221/148‑1 (MQ=255)
                                                                                   gTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCg                                                                 >  3:335270/1‑149 (MQ=255)
                                                                                    tctcGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGACCTTCCGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCg                                                                 >  3:361548/1‑148 (MQ=255)
                                                                                      tcGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCt                                                               >  3:173679/1‑148 (MQ=255)
                                                                                        gCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGCTCGAGAGCTTCGGCTCCGATGAACAGTAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTa                                                              <  7:59292/147‑1 (MQ=255)
                                                                                        gCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGc                                                                >  7:171545/1‑145 (MQ=255)
                                                                                        gCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGc                                                                <  8:171545/145‑1 (MQ=255)
                                                                                          aaGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTc                                                          >  7:267563/1‑149 (MQ=255)
                                                                                          aaGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTc                                                          <  4:335270/149‑1 (MQ=255)
                                                                                           aGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCg                                                         <  8:267563/149‑1 (MQ=255)
                                                                                              cTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGca                                                      >  7:265398/1‑149 (MQ=255)
                                                                                                 cATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCTATGATCGACGGCCGCTACTTCGGCACaa                                                   <  1:23099/149‑1 (MQ=255)
                                                                                                 cATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGcaca                                                    <  3:18090/148‑1 (MQ=255)
                                                                                                   tCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATg                                                 >  1:138443/1‑149 (MQ=255)
                                                                                                     gCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGgc                                               >  2:295286/1‑149 (MQ=255)
                                                                                                       ccACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGgcg                                              >  2:337422/1‑148 (MQ=255)
                                                                                                          cTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGa                                          <  1:24820/149‑1 (MQ=255)
                                                                                                               aGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGACCGaa                                     >  2:258597/1‑149 (MQ=255)
                                                                                                                   cgccAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGACCGAAcccc                                 <  6:31441/149‑1 (MQ=255)
                                                                                                                    gccAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGa                                          >  4:90898/1‑139 (MQ=255)
                                                                                                                    gccAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGa                                          <  3:90898/139‑1 (MQ=255)
                                                                                                                        aCGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGACCGAACCCCACGCt                            <  2:138443/149‑1 (MQ=255)
                                                                                                                                accacGGCCTACCCGTTCCTGACCATGGGCTCGGCCAACCTGATCGAGAGCTTCGGCTGCGATGAACAGATGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGACCGAACCCCACGCTGGCtcgtc                    <  5:214770/149‑1 (MQ=255)
                                                                                                                                 ccacGGCCTACCCGTTCCCGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGACCGAACCCCACGCTGGCtcgtcg                   <  6:151304/149‑1 (MQ=255)
                                                                                                                                 ccacGGCCTACCCGTTCCCGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGACCGAACCCCACGCTGGCtcgtcg                   <  6:156891/149‑1 (MQ=255)
                                                                                                                                   acGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGACCGAACCCCACGCTGGCTCGTCGCt                 <  4:361548/149‑1 (MQ=255)
                                                                                                                                           cccGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGACCGAACCCCACGCTGGCTCGTCGCTGGCCGAc          <  8:265398/148‑1 (MQ=255)
                                                                                                                                              gTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGACCGAACCCCACGCTGGCTCGTCGCTGGCCGACATAc      >  7:280288/1‑149 (MQ=255)
                                                                                                                                                  cTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGACCGAACCCCACGCTGGCTCGTCGCTGGCCGACATACGCAc  >  8:254514/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGCCGTCGATGCCTTCCTCGAAGCCGGCTTCCTCAACGCCAACCGGGACTTCGAGGTCGGCGGCATGCAGCTGCCCAGCCTGGTCTCGCAAGCCTGCATCGCCCACTTCCAGGCCGCCAACGCCGGCACCACGGCCTACCCGTTCCTGACCATGGGCGCGGCCAACCTGATCGAGAGCTTCGGCTCCGATGAACAGAAGCGCCTGTTCCTGCAACCGATGATCGACGGCCGCTACTTCGGCACAATGGCGCTGACCGAACCCCACGCTGGCTCGTCGCTGGCCGACATACGCAC  >  NC_002947/5635871‑5636165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑