| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,658,116 | T→G | 8.6% | H57Q (CAT→CAG) | sahR → | methionine metabolism transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,658,116 | 0 | T | G | 8.6% | 246.8 / 13.2 | 81 | H57Q (CAT→CAG) | sahR | methionine metabolism transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (36/38); new base G (2/5); total (38/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
CCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTAC > NC_002947/5657970‑5658264 | cccGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATc < 3:307324/148‑1 (MQ=255) ccGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCAc < 4:236379/149‑1 (MQ=255) cGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCAcc > 1:74925/1‑149 (MQ=255) gAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCt < 6:108779/149‑1 (MQ=255) aaCGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAg < 2:74925/149‑1 (MQ=255) aaCGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAg < 6:66040/149‑1 (MQ=255) gAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCt > 6:123396/1‑149 (MQ=255) gAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCt > 3:109850/1‑149 (MQ=255) aaaCATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTg < 8:30074/149‑1 (MQ=255) aCATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCAGCACCTTGAGGT‑GATGGc < 1:287173/149‑1 (MQ=255) ggCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTC‑GGCCTGAGCCAGCACCTTGAGGT‑GATGGCTCatg < 1:23237/148‑3 (MQ=38) gCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCCTGAGCCAGCACCTTGAGGT‑GATGGCTCATGc < 7:260018/149‑1 (MQ=39) cAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATCTTTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGc < 1:91816/149‑1 (MQ=255) ccTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGAcc > 4:293821/1‑149 (MQ=255) tgCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTc < 4:66446/147‑1 (MQ=255) tgCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTc < 6:380588/147‑1 (MQ=255) gCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTg > 2:281354/1‑149 (MQ=255) gCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCAGCACCTTGAGGT‑GATGGc > 6:4306/1‑133 (MQ=255) gCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCAGCACCTTGAGGT‑GATGGc < 5:4306/133‑1 (MQ=255) cAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCAGGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTg < 3:331723/148‑1 (MQ=255) aaaGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTgg > 3:103327/1‑148 (MQ=255) aaGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGc > 7:189651/1‑148 (MQ=255) aGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACccg < 5:133919/149‑1 (MQ=255) gTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACccgc > 5:106649/1‑149 (MQ=255) cgctgcgcCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAgg < 5:123396/148‑1 (MQ=255) gctgcgcCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCg > 1:224185/1‑128 (MQ=255) gctgcgcCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCg < 2:224185/128‑1 (MQ=255) cgcCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAAc < 6:347842/147‑1 (MQ=255) cTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCa > 2:364241/1‑148 (MQ=255) cTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCAt > 4:65330/1‑149 (MQ=255) aaCGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCAtctt < 6:348926/149‑1 (MQ=255) aaCGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCAtct > 2:196180/1‑148 (MQ=255) aaCGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCAtc < 6:106649/147‑1 (MQ=255) cGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCAtcttct < 5:88569/149‑1 (MQ=255) tATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGTCGCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTa < 1:281354/149‑1 (MQ=255) gcgcgcCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTAccgcc > 4:349822/1‑149 (MQ=255) gcgcCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGt > 2:311082/1‑149 (MQ=255) gcgcCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGt > 8:190722/1‑149 (MQ=255) gcgcCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGt > 8:196068/1‑149 (MQ=255) gcCTTGTCCAATGACTCGTTCGGAGTGCTGTAACTTACGCAGATC‑TACGACATCGGCCAGTAGGGCATGAGCCATCACCTCAACTT‑GCTGTATCAGTCCGACATCGTGGCAACCCTCCGCGAAGGCAAAGCAATCTAATACAGCCGtgc > 8:158338/1‑149 (MQ=255) ttGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGtgccct > 5:337866/1‑149 (MQ=255) gCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGtgccctgcc < 1:196180/149‑1 (MQ=255) gCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGtgccctgcc < 3:293821/149‑1 (MQ=255) gACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATgg > 4:337280/1‑149 (MQ=255) aCTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGc > 8:267700/1‑149 (MQ=255) cTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGcc > 2:350424/1‑149 (MQ=255) gTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGCCCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTgc > 1:187497/1‑149 (MQ=255) ggCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCt < 1:364241/149‑1 (MQ=255) ggCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCt < 7:196068/149‑1 (MQ=255) ggCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCt < 7:190722/149‑1 (MQ=255) cGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTgg < 7:267700/149‑1 (MQ=255) cGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTgg < 6:356934/149‑1 (MQ=255) cGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTgg < 6:362121/149‑1 (MQ=255) tGCTGGAACTGGCGCAGATC‑TTCGACATCGTCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGt < 8:189651/149‑1 (MQ=255) tGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGt < 4:103327/149‑1 (MQ=255) cTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTgg > 5:32289/1‑149 (MQ=255) gAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCg < 1:350424/149‑1 (MQ=255) gcAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTgg < 1:304762/134‑1 (MQ=255) gcAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTgg > 2:304762/1‑134 (MQ=255) gcAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCAcg > 8:158507/1‑149 (MQ=255) cAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCAcgc < 6:32289/149‑1 (MQ=255) aTC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACgcggc > 4:143052/1‑149 (MQ=255) tC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATg > 4:59483/1‑117 (MQ=255) tC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATg < 3:59483/117‑1 (MQ=255) cGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCg > 8:152333/1‑146 (MQ=255) ggCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaag > 6:330417/1‑148 (MQ=255) ggCCAGGCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaag < 7:158338/148‑1 (MQ=255) gCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaagaa < 5:13301/149‑1 (MQ=255) gCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaag > 7:175968/1‑147 (MQ=255) aGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTc < 4:109850/149‑1 (MQ=255) gCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCgacgac > 4:38347/1‑149 (MQ=255) tGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaa > 1:185308/1‑134 (MQ=255) tGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaa < 2:185308/134‑1 (MQ=255) gAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTgg > 4:315281/1‑149 (MQ=255) gCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTTCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTggac > 7:180532/1‑147 (MQ=255) gCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGcc < 3:315281/149‑1 (MQ=255) ccATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGTAGGCAACGCCCTCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGccc > 1:90964/1‑149 (MQ=255) ccATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaa > 7:269013/1‑130 (MQ=255) ccATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCgaa < 8:269013/130‑1 (MQ=255) ccAGCACCTTGAGGT‑GATGGCTCAGGCCGACCTCGTGGCAACGCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTc < 8:346760/136‑1 (MQ=255) ccAGCACCTTGAGGT‑GATGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTc > 7:346760/1‑136 (MQ=255) tCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTAc > 1:150133/1‑149 (MQ=255) | CCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGCGCCTTGGCCAATGACTCGTTCGGCGTGCTGGAACTGGCGCAGATC‑TTCGACATCGGCCAGTCGGGCATGAGCCATCACCTCAAGGT‑GCTGGCTCAGGCCGACCTCGTGGCAACCCGCCGCGAAGGCAACGCCATCTTCTACCGCCGTGCCCTGCCCGATGGCCTGCGCCTGGGTGGCCGGCTGCACGCGGCGCTGCTCGAAGAAGTCGACGACCTGGCCCTAC > NC_002947/5657970‑5658264 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |