| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,670,453 | G→A | 10.9% | intergenic (+42/‑43) | metF → / → PP_4978 | 5,10‑methylenetetrahydrofolate reductase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,670,453 | 0 | G | A | 10.9% | 148.0 / 7.2 | 55 | intergenic (+42/‑43) | metF/PP_4978 | 5,10‑methylenetetrahydrofolate reductase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (27/22); new base A (3/3); total (30/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01 | |||||||||||
TGTGCGAACAGCTGTTGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGT > NC_002947/5670305‑5670593 | tgtgCGAACAGCTGTTGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTg < 8:96932/149‑1 (MQ=255) cGAACAGCTGTTGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCa > 6:161843/1‑149 (MQ=255) gAACAGCTGTTGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCa > 3:66032/1‑148 (MQ=255) gCTGTTGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGAc > 3:288398/1‑148 (MQ=255) ttGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTaaa < 5:179989/148‑1 (MQ=255) ttGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTaaa > 6:179989/1‑148 (MQ=255) gCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGc < 4:288398/148‑1 (MQ=255) cAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCt > 8:54119/1‑149 (MQ=255) aaGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCt > 5:43649/1‑148 (MQ=255) aaGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCt > 5:342548/1‑148 (MQ=255) aGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTg > 2:318690/1‑130 (MQ=255) aGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTg < 1:318690/130‑1 (MQ=255) ggcggcGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTAt > 1:218495/1‑149 (MQ=255) ggcggcGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTAGTCTATGACCAAAGcc > 5:311663/1‑145 (MQ=255) ggcggcGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTAGTCTATGACCAAAGcc < 6:311663/145‑1 (MQ=255) gcgcgcCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGCGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCAt < 6:314207/149‑1 (MQ=255) ccAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCg < 3:151188/149‑1 (MQ=255) ggCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGAATAAGGCTTTAGTCTATGACCAAAGCCTTAATCAtttcttaag < 6:136294/149‑7 (MQ=37) cTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAgg < 2:91793/149‑1 (MQ=255) cTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTc < 6:43649/148‑1 (MQ=255) taCCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTtgc > 2:163786/1‑149 (MQ=255) cTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTtgcctg > 5:318485/1‑149 (MQ=255) ttGAACCAGGCTGAACCGACCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTTGCTAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAATCCTTATTCATTTTCGGGTTAAACAGGTCTtgcctgc < 7:56587/149‑1 (MQ=255) cAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATgca < 1:342916/149‑1 (MQ=255) cAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATg > 5:66074/1‑147 (MQ=255) ggCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCtt < 2:132765/135‑1 (MQ=255) ggCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCtt > 1:132765/1‑135 (MQ=255) aGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAAcc < 6:318485/149‑1 (MQ=255) ccTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCtt > 5:212393/1‑149 (MQ=255) tGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTcg > 1:176949/1‑149 (MQ=255) tGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCca > 3:33131/1‑147 (MQ=255) acCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGta < 1:184287/149‑1 (MQ=255) ccTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGtat < 5:120831/149‑1 (MQ=255) ccTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGta > 6:120831/1‑148 (MQ=255) cgcgCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCtttt < 6:317013/149‑1 (MQ=255) cgcTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTATAGCCTTATTCATTTTCGGGTTAAAGAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTgg < 2:286360/149‑1 (MQ=255) aaaTCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCgg > 3:46281/1‑149 (MQ=255) aTCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGc > 2:141448/1‑148 (MQ=255) aTCTTTTGGCAAAGTCTGCCTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGc > 4:323120/1‑148 (MQ=255) ttGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAAc < 5:161843/146‑1 (MQ=255) tGGCAAAGTCTGCCTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTa < 3:323120/148‑1 (MQ=255) gTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCCCAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAg > 2:227180/1‑148 (MQ=255) gTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGACTGCCATGCAGCACACCCAACCTTCGCGCCCAAAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACcagcag > 2:227185/1‑148 (MQ=255) cTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGACCGGCAACCTACCATCAGGaa < 4:66032/146‑1 (MQ=255) ttAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCt > 6:52980/1‑149 (MQ=255) aaGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTg < 2:176949/148‑1 (MQ=255) gAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCtt < 4:46281/149‑1 (MQ=255) gaatAAGGCTTTAGTCTATGACCAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCCGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACGATCAGGAAGCCTGCTTCAGc > 6:264007/4‑148 (MQ=38) aTTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCACCCTACCATCAGGAAGCCTGCTTCAGCAt > 8:291094/1‑149 (MQ=255) aTTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCAt > 6:166512/1‑149 (MQ=255) ttAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCCGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCAGGCTTcctgatg < 6:342548/149‑7 (MQ=255) ttAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATc < 4:33131/149‑1 (MQ=255) ttAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATc > 5:11368/1‑149 (MQ=255) aaGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGt > 5:128982/1‑149 (MQ=255) aGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGt < 7:54119/148‑1 (MQ=255) ggCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGt > 8:298615/1‑147 (MQ=255) ggCTTTAGTCTATGACCAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGaa < 2:170493/130‑1 (MQ=255) ggCTTTAGTCTATGACCAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGaa > 1:170493/1‑130 (MQ=255) | TGTGCGAACAGCTGTTGCAAGGCGGCGCGCCAGGCCTGCACTTCTATACCTTGAACCAGGCTGAACCGAGCCTGGCGATCTGGAACAACCTGAAGCTGCCGCGCTGAAATCTTTTGGCAAAGTCTGCTTAAGAAATGATTAAGGCTTTGGTCATAGACTAAAGCCTTATTCATTTTCGGGTTAAACAGGTCTTGCCTGCCATGCAGCACACCCAACCTTCGCGCCCACAGCTTGTATACCTGGCTTTTGGCCCGGCAACCTACCATCAGGAAGCCTGCTTCAGCATCGT > NC_002947/5670305‑5670593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |