Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,679,142 G→T 7.5% E256D (GAG→GAT)  bioA → adenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,679,1420GT 7.5% 167.3 / 4.1 53E256D (GAG→GAT) bioAadenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base T (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

CCCTGGCCGAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGT  >  NC_002947/5678994‑5679290
                                                                                                                                                    |                                                                                                                                                     
cccTGGCCGAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACgag                                                                                                                                                       <  4:149738/149‑1 (MQ=255)
 ccTGGCCGAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACgaga                                                                                                                                                      <  1:21287/149‑1 (MQ=255)
            accacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGc                                                                                                                                           <  8:89389/149‑1 (MQ=255)
             ccacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCt                                                                                                                                          <  6:302592/149‑1 (MQ=255)
              cacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCtt                                                                                                                                         >  1:183265/1‑149 (MQ=255)
              cacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCtt                                                                                                                                         >  7:101444/1‑149 (MQ=255)
              cacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCtt                                                                                                                                         >  5:234132/1‑149 (MQ=255)
               acGCCTCGATCAGCGCCGTGATCGTCGAGCCGATGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGGCGATCTCGTCGTggataag                                                                                                                                        <  1:97429/149‑6 (MQ=255)
                 gCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCgg                                                                                                                                      <  7:23342/149‑1 (MQ=255)
                        tCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCAcc                                                                                                                               <  5:323133/149‑1 (MQ=255)
                            cgcCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCa                                                                                                                           >  2:98812/1‑149 (MQ=255)
                              ccGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACg                                                                                                                         <  2:93906/149‑1 (MQ=255)
                                    tcgtcgAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTc                                                                                                                   <  1:98812/149‑1 (MQ=255)
                                     cgtcgAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCg                                                                                                                  >  6:378806/1‑149 (MQ=255)
                                              gCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAg                                                                                                          >  8:227360/1‑148 (MQ=255)
                                               cTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGc                                                                                                         >  2:309582/1‑148 (MQ=255)
                                                  aTACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCaa                                                                                                       <  4:5487/147‑1 (MQ=255)
                                                              ggTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGccc                                                                                         <  6:234132/149‑1 (MQ=255)
                                                              ggTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGccc                                                                                         <  1:309582/149‑1 (MQ=255)
                                                                  gCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGAt                                                                                     <  1:134047/149‑1 (MQ=255)
                                                                  gCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGAt                                                                                     <  8:101444/149‑1 (MQ=255)
                                                                  gCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGAt                                                                                     >  3:76468/1‑149 (MQ=255)
                                                                  gCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGAt                                                                                     <  2:183265/149‑1 (MQ=255)
                                                                        gcATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtg                                                                               >  5:358013/1‑149 (MQ=255)
                                                                          aTGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgtg                                                                             <  7:227360/149‑1 (MQ=255)
                                                                              accaccCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTg                                                                         >  6:269149/1‑149 (MQ=255)
                                                                              accaccCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTg                                                                         >  2:35350/1‑149 (MQ=255)
                                                                               ccaccCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGt                                                                        <  1:35350/149‑1 (MQ=255)
                                                                                   ccGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTcc                                                                      >  4:296642/1‑147 (MQ=255)
                                                                                     ggTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAgg                                                                  <  5:378806/149‑1 (MQ=255)
                                                                                            cTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGAc                                                           >  3:291667/1‑149 (MQ=255)
                                                                                            cTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGAc                                                           >  2:195665/1‑149 (MQ=255)
                                                                                             tCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGAcc                                                          >  5:180898/1‑149 (MQ=255)
                                                                                             tCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGAcc                                                          >  1:164788/1‑149 (MQ=255)
                                                                                                aGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACcgg‑c                                                      <  6:358013/149‑1 (MQ=255)
                                                                                                         gcgAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTg                                             >  8:327765/1‑149 (MQ=255)
                                                                                                          cgAGGCCTGCGACCGCTATGACGTGCACCTTCTCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGc                                            <  2:150917/149‑1 (MQ=255)
                                                                                                                cTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTgg                                      <  1:195665/149‑1 (MQ=255)
                                                                                                                 tGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGgc                                     >  1:123175/1‑149 (MQ=255)
                                                                                                                   cGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGgc                                     <  4:291667/147‑1 (MQ=255)
                                                                                                                    gACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGgccgc                                  >  4:353526/1‑149 (MQ=255)
                                                                                                                         cTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCgcctgcc                             >  5:18028/1‑149 (MQ=255)
                                                                                                                         cTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCgcctgcc                             >  6:33238/1‑149 (MQ=255)
                                                                                                                            tGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGa                          <  6:18028/149‑1 (MQ=255)
                                                                                                                             gACGTGCACCTTATCCACGACGAGATTGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGac                         >  2:266042/1‑149 (MQ=255)
                                                                                                                                    aCCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGa                   >  6:137845/1‑148 (MQ=255)
                                                                                                                                         aTCCACGACGAGATCGCCGTGGTCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCGGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGTCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAg               <  7:226608/147‑1 (MQ=255)
                                                                                                                                         aTCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAg               <  3:296642/147‑1 (MQ=255)
                                                                                                                                          tCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgt             <  1:71057/149‑1 (MQ=255)
                                                                                                                                           ccACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGAc                  >  3:313332/1‑142 (MQ=255)
                                                                                                                                           ccACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGAc                  <  4:313332/142‑1 (MQ=255)
                                                                                                                                           ccACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgtg            >  7:153635/1‑148 (MQ=255)
                                                                                                                                             acgacgAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTAc         <  6:180898/149‑1 (MQ=255)
                                                                                                                                              cgacgaGATGGCCGTTGGCTTCGGCCGCACCGTCACGATGTTCGCCTGCGAGCAAGCCGTCATCCTCCCGGATTTCCTGTGCCTGTCCAAGGCCCTTACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTAcc        <  3:259635/149‑1 (MQ=255)
                                                                                                                                                 cgaGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAgg     >  7:155523/1‑149 (MQ=255)
                                                                                                                                                    gaTGGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTAGCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGt  <  1:266042/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCCTGGCCGAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGT  >  NC_002947/5678994‑5679290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑