Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,679,147 C→T 7.1% A258V (GCC→GTC)  bioA → adenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,679,1470CT 7.1% 165.9 / 2.0 56A258V (GCC→GTC) bioAadenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA
Reads supporting (aligned to +/- strand):  ref base C (24/28);  new base T (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

ACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGTTCT  >  NC_002947/5679006‑5679293
                                                                                                                                             |                                                                                                                                                   
accacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGc                                                                                                                                              <  8:89389/149‑1 (MQ=255)
 ccacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCt                                                                                                                                             <  6:302592/149‑1 (MQ=255)
  cacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCtt                                                                                                                                            >  1:183265/1‑149 (MQ=255)
  cacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCtt                                                                                                                                            >  7:101444/1‑149 (MQ=255)
  cacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCtt                                                                                                                                            >  5:234132/1‑149 (MQ=255)
   acGCCTCGATCAGCGCCGTGATCGTCGAGCCGATGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGGCGATCTCGTCGTggataag                                                                                                                                           <  1:97429/149‑6 (MQ=255)
     gCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCgg                                                                                                                                         <  7:23342/149‑1 (MQ=255)
            tCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCAcc                                                                                                                                  <  5:323133/149‑1 (MQ=255)
                cgcCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCa                                                                                                                              >  2:98812/1‑149 (MQ=255)
                  ccGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACg                                                                                                                            <  2:93906/149‑1 (MQ=255)
                        tcgtcgAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTc                                                                                                                      <  1:98812/149‑1 (MQ=255)
                         cgtcgAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCg                                                                                                                     >  6:378806/1‑149 (MQ=255)
                                  gCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAg                                                                                                             >  8:227360/1‑148 (MQ=255)
                                   cTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGc                                                                                                            >  2:309582/1‑148 (MQ=255)
                                      aTACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCaa                                                                                                          <  4:5487/147‑1 (MQ=255)
                                                  ggTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGccc                                                                                            <  1:309582/149‑1 (MQ=255)
                                                  ggTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGccc                                                                                            <  6:234132/149‑1 (MQ=255)
                                                      gCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGAt                                                                                        <  1:134047/149‑1 (MQ=255)
                                                      gCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGAt                                                                                        <  8:101444/149‑1 (MQ=255)
                                                      gCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGAt                                                                                        <  2:183265/149‑1 (MQ=255)
                                                      gCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGAt                                                                                        >  3:76468/1‑149 (MQ=255)
                                                            gcATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtg                                                                                  >  5:358013/1‑149 (MQ=255)
                                                              aTGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgtg                                                                                <  7:227360/149‑1 (MQ=255)
                                                                  accaccCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTg                                                                            >  2:35350/1‑149 (MQ=255)
                                                                  accaccCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTg                                                                            >  6:269149/1‑149 (MQ=255)
                                                                   ccaccCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGt                                                                           <  1:35350/149‑1 (MQ=255)
                                                                       ccGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTcc                                                                         >  4:296642/1‑147 (MQ=255)
                                                                         ggTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAgg                                                                     <  5:378806/149‑1 (MQ=255)
                                                                                cTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGAc                                                              >  2:195665/1‑149 (MQ=255)
                                                                                cTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGAc                                                              >  3:291667/1‑149 (MQ=255)
                                                                                 tCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGAcc                                                             >  5:180898/1‑149 (MQ=255)
                                                                                 tCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGAcc                                                             >  1:164788/1‑149 (MQ=255)
                                                                                    aGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACcgg‑c                                                         <  6:358013/149‑1 (MQ=255)
                                                                                             gcgAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTg                                                >  8:327765/1‑149 (MQ=255)
                                                                                              cgAGGCCTGCGACCGCTATGACGTGCACCTTCTCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGc                                               <  2:150917/149‑1 (MQ=255)
                                                                                                    cTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTgg                                         <  1:195665/149‑1 (MQ=255)
                                                                                                     tGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGgc                                        >  1:123175/1‑149 (MQ=255)
                                                                                                       cGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGgc                                        <  4:291667/147‑1 (MQ=255)
                                                                                                        gACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGgccgc                                     >  4:353526/1‑149 (MQ=255)
                                                                                                             cTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCgcctgcc                                >  5:18028/1‑149 (MQ=255)
                                                                                                             cTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCgcctgcc                                >  6:33238/1‑149 (MQ=255)
                                                                                                                tGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGa                             <  6:18028/149‑1 (MQ=255)
                                                                                                                 gACGTGCACCTTATCCACGACGAGATTGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGac                            >  2:266042/1‑149 (MQ=255)
                                                                                                                        aCCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGa                      >  6:137845/1‑148 (MQ=255)
                                                                                                                             aTCCACGACGAGATCGCCGTGGTCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCGGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGTCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAg                  <  7:226608/147‑1 (MQ=255)
                                                                                                                             aTCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAg                  <  3:296642/147‑1 (MQ=255)
                                                                                                                              tCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgt                <  1:71057/149‑1 (MQ=255)
                                                                                                                               ccACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGAc                     >  3:313332/1‑142 (MQ=255)
                                                                                                                               ccACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGAc                     <  4:313332/142‑1 (MQ=255)
                                                                                                                               ccACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgtg               >  7:153635/1‑148 (MQ=255)
                                                                                                                                 acgacgAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTAc            <  6:180898/149‑1 (MQ=255)
                                                                                                                                  cgacgaGATGGCCGTTGGCTTCGGCCGCACCGTCACGATGTTCGCCTGCGAGCAAGCCGTCATCCTCCCGGATTTCCTGTGCCTGTCCAAGGCCCTTACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTAcc           <  3:259635/149‑1 (MQ=255)
                                                                                                                                     cgaGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAgg        >  7:155523/1‑149 (MQ=255)
                                                                                                                                        gaTGGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTAGCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGt     <  1:266042/149‑1 (MQ=255)
                                                                                                                                          tCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGTTc   <  6:116832/149‑1 (MQ=255)
                                                                                                                                            gCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGTTCt  <  5:137845/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
ACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGG‑CGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGTTCT  >  NC_002947/5679006‑5679293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑