Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,692,104	C→G	6.4%	S160W (TCG→TGG) ‡	PP_4994 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,692,104	0	C	G	6.4%	195.7 / 3.9	63	S160W (TCG→TGG) ‡	PP_4994	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (30/29);  new base G (2/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01

CGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTGGCGCAAG  >  NC_002947/5691957‑5692249                                                                                                                                                     |                                                                                                                                                  aggCCACGCCCGCCGCGCTCCCACCCCCACCCCCCAAAAAGTCGCCACTGGCCCCGACGGCGCCCAAGCCGCACAACGTCCAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCg                                                                                                                                                  <  4:82777/148‑1 (MQ=255)  ggCCAGGCCCGAGGTCGTCCTACCCCCTCCCCCGAAAAAGTCGGCAGTGGGGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGc                                                                                                                                                 <  7:352327/149‑1 (MQ=255)       ggCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAATGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTg                                                                                                                                            <  1:285081/149‑1 (MQ=255)            gAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGACCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTGCGAGCTGTcga                                                                                                                                        <  1:271987/149‑3 (MQ=255)               gtggtCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTccagcca                                                                                                                                    <  5:131612/149‑1 (MQ=255)                     ccACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTGCGAGCTGTcgaa                                                                                                                                       <  5:158310/140‑4 (MQ=255)                     ccACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTGCGAGCTGTcgaa                                                                                                                                       >  6:158310/1‑137 (MQ=255)                        cccccTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAg                                                                                                                           >  6:144969/1‑149 (MQ=255)                         ccccTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGc                                                                                                                          >  8:164779/1‑149 (MQ=255)                         ccccTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGc                                                                                                                          >  7:228099/1‑149 (MQ=255)                               ccccGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAg                                                                                                                    >  5:253819/1‑149 (MQ=255)                               ccccGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGACCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGa                                                                                                                     <  1:301307/148‑1 (MQ=255)                                cccGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAgg                                                                                                                   >  1:297239/1‑149 (MQ=255)                                cccGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAgg                                                                                                                   >  7:346233/1‑149 (MQ=255)                                         gTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGCCTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGt                                                                                                          >  3:215818/1‑149 (MQ=255)                                         gTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCACAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGt                                                                                                          <  5:94867/149‑1 (MQ=255)                                              cAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAAc                                                                                                     <  8:228099/149‑1 (MQ=255)                                              cAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAAc                                                                                                     <  7:164779/149‑1 (MQ=255)                                                      acgacgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGcc                                                                                                                         <  6:10923/121‑1 (MQ=255)                                                      acgacgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGcc                                                                                                                         >  5:10923/1‑121 (MQ=255)                                                      acgacgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAgcagca                                                                                             <  7:264256/149‑1 (MQ=255)                                                       cgacgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAgcagca                                                                                             <  8:346233/148‑1 (MQ=255)                                                        gacgGCGCCCAAGCCGCACAAGGTCGAGCCCAACCCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGt                                                                                                          >  2:159859/1‑134 (MQ=255)                                                        gacgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGt                                                                                                          <  1:159859/134‑1 (MQ=255)                                                        gacgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCACCGagcag                                                                                               >  3:273362/1‑145 (MQ=255)                                                          cgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAg                                                                                                              >  6:224591/1‑128 (MQ=255)                                                          cgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAg                                                                                                              <  5:224591/128‑1 (MQ=255)                                                                ccAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTccagc                                                                                                                                      <  6:125860/98‑1 (MQ=255)                                                                ccAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTccagc                                                                                                                                      >  5:125860/1‑98 (MQ=255)                                                                ccAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGc                                                                                    >  3:351061/1‑148 (MQ=255)                                                                 cAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGcc                                                                                   >  8:5868/1‑148 (MQ=255)                                                                         acaAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCcg                                                                          <  1:51574/149‑1 (MQ=255)                                                                             ggTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGca                                                                      >  8:241639/1‑149 (MQ=255)                                                                               tCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGcatc                                                                    <  7:241639/149‑1 (MQ=255)                                                                                    cccAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCGAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCg                                                               >  2:72610/1‑149 (MQ=255)                                                                                     ccAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGTTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGc                                                              >  5:267915/1‑149 (MQ=255)                                                                                       aaaCCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCTAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCt                                                            <  1:72610/149‑1 (MQ=255)                                                                                       aaaCCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCt                                                            >  4:58902/1‑149 (MQ=255)                                                                                          cccAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGaa                                                         >  1:69955/1‑149 (MQ=255)                                                                                             aaGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAAcgc                                                      >  5:114077/1‑149 (MQ=255)                                                                                               ggAAAGCAAGGCGCCG‑CACAAGCCCGCCACGCCGACGGCCGACTTCGACAGCTCGCAGCTGTCCAGCCTGATCGCCAGCCTGGAGCCGGAGCAGTCCAACGAGCAGCAGATGTACGCAAAGCGCCCGCTCATCCAGCGGCTGAccgccg                                                    >  6:16769/1‑149 (MQ=255)                                                                                                        ggCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCa                                           >  4:61823/1‑149 (MQ=255)                                                                                                                 ccAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGAc                                   <  6:114077/149‑1 (MQ=255)                                                                                                                  cAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGAc                                   >  6:371609/1‑148 (MQ=255)                                                                                                                       cagccGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGgc                             <  3:61823/149‑1 (MQ=255)                                                                                                                          ccGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGcagcag                                                                                            <  3:346830/83‑1 (MQ=255)                                                                                                                          ccGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGcagcag                                                                                            >  4:346830/1‑83 (MQ=255)                                                                                                                           cgcgCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCt                         <  5:144969/149‑1 (MQ=255)                                                                                                                                cGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTAc                    <  6:253819/149‑1 (MQ=255)                                                                                                                                  aCGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCCGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACaa                  <  5:371609/149‑1 (MQ=255)                                                                                                                                    gCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAg                 >  7:189424/1‑148 (MQ=255)                                                                                                                                    gCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAg                 <  8:189424/148‑1 (MQ=255)                                                                                                                                      cGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAgg                >  3:201987/1‑147 (MQ=255)                                                                                                                                          cTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTgg                       >  6:206945/1‑136 (MQ=255)                                                                                                                                          cTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTgg                       <  5:206945/136‑1 (MQ=255)                                                                                                                                           ttCGACAGCTCGCAGCTGTCCAGCCAGCTCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTg         >  5:245764/1‑149 (MQ=255)                                                                                                                                            tggACAGCTGCGAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTgg        >  5:69993/3‑149 (MQ=255)                                                                                                                                             cGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGTTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTgg        <  6:267915/148‑1 (MQ=255)                                                                                                                                              gACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTGgc       <  2:203343/148‑1 (MQ=255)                                                                                                                                                   cTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGcatc                                                                    <  4:316116/82‑1 (MQ=255)                                                                                                                                                    tCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTGgcgc     <  1:275558/144‑1 (MQ=255)                                                                                                                                                    tCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTGgcgc     >  2:275558/1‑144 (MQ=255)                                                                                                                                                    tCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTGGCGCaag  >  5:192996/1‑147 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTGGCGCAAG  >  NC_002947/5691957‑5692249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑