Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,692,105	G→C	6.5%	S160S (TCG→TCC) ‡	PP_4994 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,692,105	0	G	C	6.5%	189.7 / 2.9	62	S160S (TCG→TCC) ‡	PP_4994	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (30/27);  new base C (2/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

CGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTGGCGCAAGAAG  >  NC_002947/5691957‑5692252                                                                                                                                                      |                                                                                                                                                    aggCCACGCCCGCCGCGCTCCCACCCCCACCCCCCAAAAAGTCGCCACTGGCCCCGACGGCGCCCAAGCCGCACAACGTCCAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCg                                                                                                                                                     <  4:82777/148‑1 (MQ=255)  ggCCAGGCCCGAGGTCGTCCTACCCCCTCCCCCGAAAAAGTCGGCAGTGGGGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGc                                                                                                                                                    <  7:352327/149‑1 (MQ=255)       ggCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAATGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTg                                                                                                                                               <  1:285081/149‑1 (MQ=255)            gAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAGACCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTGCGAGCTGTcga                                                                                                                                           <  1:271987/149‑3 (MQ=255)               gtggtCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTccagcca                                                                                                                                       <  5:131612/149‑1 (MQ=255)                     ccACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTGCGAGCTGTcgaa                                                                                                                                          <  5:158310/140‑4 (MQ=255)                     ccACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTGCGAGCTGTcgaa                                                                                                                                          >  6:158310/1‑137 (MQ=255)                        cccccTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAg                                                                                                                              >  6:144969/1‑149 (MQ=255)                         ccccTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGc                                                                                                                             >  8:164779/1‑149 (MQ=255)                         ccccTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGc                                                                                                                             >  7:228099/1‑149 (MQ=255)                               ccccGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAg                                                                                                                       >  5:253819/1‑149 (MQ=255)                               ccccGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGACCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGa                                                                                                                        <  1:301307/148‑1 (MQ=255)                                cccGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAgg                                                                                                                      >  7:346233/1‑149 (MQ=255)                                cccGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAgg                                                                                                                      >  1:297239/1‑149 (MQ=255)                                         gTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGCCTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGt                                                                                                             >  3:215818/1‑149 (MQ=255)                                         gTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCACAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGt                                                                                                             <  5:94867/149‑1 (MQ=255)                                              cAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAAc                                                                                                        <  7:164779/149‑1 (MQ=255)                                              cAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAAc                                                                                                        <  8:228099/149‑1 (MQ=255)                                                      acgacgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGcc                                                                                                                            <  6:10923/121‑1 (MQ=255)                                                      acgacgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGcc                                                                                                                            >  5:10923/1‑121 (MQ=255)                                                      acgacgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAgcagca                                                                                                <  7:264256/149‑1 (MQ=255)                                                       cgacgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAgcagca                                                                                                <  8:346233/148‑1 (MQ=255)                                                        gacgGCGCCCAAGCCGCACAAGGTCGAGCCCAACCCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGt                                                                                                             >  2:159859/1‑134 (MQ=255)                                                        gacgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGt                                                                                                             <  1:159859/134‑1 (MQ=255)                                                        gacgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCACCGagcag                                                                                                  >  3:273362/1‑145 (MQ=255)                                                          cgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAg                                                                                                                 <  5:224591/128‑1 (MQ=255)                                                          cgGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAg                                                                                                                 >  6:224591/1‑128 (MQ=255)                                                                ccAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTccagc                                                                                                                                         <  6:125860/98‑1 (MQ=255)                                                                ccAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTccagc                                                                                                                                         >  5:125860/1‑98 (MQ=255)                                                                ccAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGc                                                                                       >  3:351061/1‑148 (MQ=255)                                                                 cAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGcc                                                                                      >  8:5868/1‑148 (MQ=255)                                                                         acaAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCcg                                                                             <  1:51574/149‑1 (MQ=255)                                                                             ggTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGca                                                                         >  8:241639/1‑149 (MQ=255)                                                                               tCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGcatc                                                                       <  7:241639/149‑1 (MQ=255)                                                                                    cccAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCGAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCg                                                                  >  2:72610/1‑149 (MQ=255)                                                                                     ccAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGTTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGc                                                                 >  5:267915/1‑149 (MQ=255)                                                                                       aaaCCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCTAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCt                                                               <  1:72610/149‑1 (MQ=255)                                                                                       aaaCCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCt                                                               >  4:58902/1‑149 (MQ=255)                                                                                          cccAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGaa                                                            >  1:69955/1‑149 (MQ=255)                                                                                             aaGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAAcgc                                                         >  5:114077/1‑149 (MQ=255)                                                                                               ggAAAGCAAGGCGCCG‑CACAAGCCCGCCACGCCGACGGCCGACTTCGACAGCTCGCAGCTGTCCAGCCTGATCGCCAGCCTGGAGCCGGAGCAGTCCAACGAGCAGCAGATGTACGCAAAGCGCCCGCTCATCCAGCGGCTGAccgccg                                                       >  6:16769/1‑149 (MQ=255)                                                                                                        ggCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCa                                              >  4:61823/1‑149 (MQ=255)                                                                                                                 ccAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGAc                                      <  6:114077/149‑1 (MQ=255)                                                                                                                  cAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGAc                                      >  6:371609/1‑148 (MQ=255)                                                                                                                       cagccGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGgc                                <  3:61823/149‑1 (MQ=255)                                                                                                                          ccGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGcagcag                                                                                               >  4:346830/1‑83 (MQ=255)                                                                                                                          ccGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGcagcag                                                                                               <  3:346830/83‑1 (MQ=255)                                                                                                                           cgcgCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCt                            <  5:144969/149‑1 (MQ=255)                                                                                                                                cGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTAc                       <  6:253819/149‑1 (MQ=255)                                                                                                                                  aCGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCCGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACaa                     <  5:371609/149‑1 (MQ=255)                                                                                                                                    gCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAg                    <  8:189424/148‑1 (MQ=255)                                                                                                                                    gCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAg                    >  7:189424/1‑148 (MQ=255)                                                                                                                                      cGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAgg                   >  3:201987/1‑147 (MQ=255)                                                                                                                                          cTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTgg                          <  5:206945/136‑1 (MQ=255)                                                                                                                                          cTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTgg                          >  6:206945/1‑136 (MQ=255)                                                                                                                                           ttCGACAGCTCGCAGCTGTCCAGCCAGCTCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTg            >  5:245764/1‑149 (MQ=255)                                                                                                                                            tggACAGCTGCGAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTgg           >  5:69993/3‑149 (MQ=255)                                                                                                                                             cGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGTTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTgg           <  6:267915/148‑1 (MQ=255)                                                                                                                                              gACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTGgc          <  2:203343/148‑1 (MQ=255)                                                                                                                                                   cTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGcatc                                                                       <  4:316116/82‑1 (MQ=255)                                                                                                                                                    tCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTGgcgc        >  2:275558/1‑144 (MQ=255)                                                                                                                                                    tCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTGgcgc        <  1:275558/144‑1 (MQ=255)                                                                                                                                                    tCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTGGCGCaag     >  5:192996/1‑147 (MQ=255)                                                                                                                                                      gCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTGGCGCaagaag  <  4:215818/148‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    CGGCCAGGCCCGAGGTGGTCCCACCCCCTCCCCCGAAAAAGTCGGCAGTGGTGACGACGGCGCCCAAGCCGCACAAGGTCGAGCCCAAACCCAAGGAAAGCAAGGCGCAG‑CCCAAGCCAGCCGCGCCGACGCCGGACTTCGACAGCTCGCAGCTGTCCAGCCAGATCGCCAGCCTGGAGGCGGAGCTGTCCAACGAGCAGCAGATGTACGCCAAGCGCCCGCGCATCCATCGCCTGAACGCCGCATCGACCATGCGCGACAAGGGCGCCTGGTACAAGGAAGAGTGGCGCAAGAAG  >  NC_002947/5691957‑5692252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑