Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	428,748	G→A	5.9%	L87L (CTG→TTG)	PP_0351 ←	transmembrane sensor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	428,748	0	G	A	5.9%	205.2 / 2.3	67	L87L (CTG→TTG)	PP_0351	transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base G (33/30);  new base A (2/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

GTTCAGGCTCAGCAGTGTGCCGTCTTCTAGGCGCAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCAT  >  NC_002947/428602‑428894                                                                                                                                                   |                                                                                                                                                   gtTCAGGCTCAGCAGTGTGCCGTCTTCTAGGCGCAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGcc                                                                                                                                                  <  8:261826/149‑1 (MQ=255)             cagTGTGCCGTCTTCTAGGCGCAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCt                                                                                                                                      >  1:132275/1‑149 (MQ=255)               gtgtGCCGTCTTCTAGGCGCAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTg                                                                                                                                    <  4:74974/149‑1 (MQ=255)               gtgtGCCGTCTTCTAGGCGCAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTg                                                                                                                                    <  6:137097/149‑1 (MQ=255)                    ccGTCTTCTAGGCGCAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAAt                                                                                                                               <  2:132275/149‑1 (MQ=255)                       tcttctAGGCGCAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAACCCGCCGCCAACCTTGCCAATGCg                                                                                                                            >  4:269181/1‑149 (MQ=255)                       tcttctAGGCGCAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAACCCGCCGCCAACCTTGCCAATGCg                                                                                                                            >  2:40704/1‑149 (MQ=255)                         ttctAGGCGCAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTc                                                                                                                          >  3:62376/1‑149 (MQ=255)                            tAGGCGCAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTcgcc                                                                                                                       >  3:28640/1‑149 (MQ=255)                            tAGGCGCAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTcgcc                                                                                                                       >  2:333712/1‑149 (MQ=255)                            tAGGCGCAAGGTGCGTAATTCACACGTGGCGGAGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCTGCCAGCCCGCCGCAAACCTTGCCAATGCGTcgcc                                                                                                                       >  3:54332/1‑149 (MQ=255)                               gcgcAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTcgccg                                                                                                                      >  2:229576/1‑147 (MQ=255)                                cgcAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCAAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAg                                                                                                                   <  4:274133/149‑1 (MQ=255)                                 gcAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAgg                                                                                                                  >  8:254400/1‑149 (MQ=255)                                 gcAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAgg                                                                                                                  >  8:40622/1‑149 (MQ=255)                                      gTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGgc                                                                                                             <  7:40622/149‑1 (MQ=255)                                      gTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGgc                                                                                                             <  8:103244/149‑1 (MQ=255)                                      gTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGgc                                                                                                             >  1:184821/1‑149 (MQ=255)                                         cGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGgcgcg                                                                                                          >  5:355712/1‑149 (MQ=255)                                                 aCCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGgcgc                                                                                                           <  6:234633/140‑1 (MQ=255)                                                 aCCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGgcgc                                                                                                           >  5:234633/1‑140 (MQ=255)                                                 aCCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTgca                                                                                                  <  5:5238/149‑1 (MQ=255)                                                   ccGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTg                                                                                                    <  1:155304/145‑1 (MQ=255)                                                   ccGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTg                                                                                                    >  2:155304/1‑145 (MQ=255)                                                     gTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGcagcag                                                                                              >  2:266785/1‑149 (MQ=255)                                                           gTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAACCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTg                                                                                        <  1:40704/149‑1 (MQ=255)                                                           gTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAACCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTg                                                                                        <  3:269181/149‑1 (MQ=255)                                                                   gTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGcc                                                                                 <  8:175023/148‑1 (MQ=255)                                                                   gTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGc                                                                                  >  3:269713/1‑147 (MQ=255)                                                                   gTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCg                                                                                >  3:113110/1‑149 (MQ=255)                                                                        ccagccagTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCa                                                                           <  2:22617/149‑1 (MQ=255)                                                                           gccagTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCt                                                                        >  4:333486/1‑149 (MQ=255)                                                                             cagTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGc                                                                      >  8:321363/1‑149 (MQ=255)                                                                               gTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCcg                                                                    <  1:333712/149‑1 (MQ=255)                                                                               gTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCcg                                                                    >  7:301665/1‑149 (MQ=255)                                                                                tAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCAGGCCAGCTGccac                                                                   <  4:269713/149‑3 (MQ=255)                                                                                  gCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCcgcg                                                                  <  7:156163/148‑1 (MQ=255)                                                                                      ggTGCCCTCGACGGGGCGCCGCCCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGGCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAAc                                                             <  8:148772/149‑1 (MQ=255)                                                                                          ccAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGt                                                         <  4:62376/149‑1 (MQ=255)                                                                                            aGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTg                                                       <  6:355712/149‑1 (MQ=255)                                                                                                   gggCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATg                                                <  7:321363/149‑1 (MQ=255)                                                                                                    ggCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATgg                                               <  8:174906/149‑1 (MQ=255)                                                                                                    ggCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATgg                                               >  7:174906/1‑149 (MQ=255)                                                                                                     gcgcCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGgc                                              <  4:54332/149‑1 (MQ=255)                                                                                                          gACCCATGCAAGCAGCGCGCCTAGCATAAACGTGCCGCCCAGCCCGCCGCAAACCTTGCCAATGCGGCGCCGAAGGCTGGCGCGCGATTGCAGCAGCGCCTGCCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGa                                         <  1:266785/149‑1 (MQ=255)                                                                                                             ccATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCt                                      >  7:347980/1‑149 (MQ=255)                                                                                                             ccATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCt                                      <  7:251388/149‑1 (MQ=255)                                                                                                               aTGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCGAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTgg                                    >  3:24291/1‑149 (MQ=255)                                                                                                                tGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGgc                                   <  1:229576/149‑1 (MQ=255)                                                                                                                   cagcagCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGgcgc                                 >  1:278297/1‑148 (MQ=255)                                                                                                                   cagcagCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGgcg                                  >  6:337800/1‑147 (MQ=255)                                                                                                                   cagcagCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGgcg                                  <  5:337800/147‑1 (MQ=255)                                                                                                                   cagcagCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGcc                                <  7:254400/149‑1 (MQ=255)                                                                                                                   cagcagCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGcc                                >  2:227450/1‑149 (MQ=255)                                                                                                                                    gAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGCGCTGGCGCCCGGCACGTGCGTGctcg               >  8:298694/1‑148 (MQ=255)                                                                                                                                    gAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCAcgtgcgtg                   >  1:97861/1‑145 (MQ=255)                                                                                                                                    gAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCAcgtgcgtg                   <  2:97861/145‑1 (MQ=255)                                                                                                                                    gAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGctct               >  6:274353/1‑149 (MQ=255)                                                                                                                                    gAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGctct               >  3:173404/1‑149 (MQ=255)                                                                                                                                       aCGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCg            >  3:3689/1‑149 (MQ=255)                                                                                                                                          tGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTg         <  3:234071/149‑1 (MQ=255)                                                                                                                                          tGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTg         <  3:333486/149‑1 (MQ=255)                                                                                                                                             cGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTggcg      <  7:298694/149‑1 (MQ=255)                                                                                                                                             cGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTggcg      >  3:198051/1‑149 (MQ=255)                                                                                                                                             cGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATT‑‑‑GCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCa   >  8:58757/1‑149 (MQ=255)                                                                                                                                               gCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTggcgg     >  4:347764/1‑148 (MQ=255)                                                                                                                                                 cAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCAt  >  3:282754/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GTTCAGGCTCAGCAGTGTGCCGTCTTCTAGGCGCAAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCAT  >  NC_002947/428602‑428894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑