Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,728,843 A→T 8.6% intergenic (‑206/+118) opgG ← / ← dtd glucans biosynthesis protein G/D‑tyrosyl‑tRNA(Tyr) deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,728,8430AT 8.6% 189.3 / 5.7 69intergenic (‑206/+118)opgG/dtdglucans biosynthesis protein G/D‑tyrosyl‑tRNA(Tyr) deacylase
Reads supporting (aligned to +/- strand):  ref base A (29/34);  new base T (4/2);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGAGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCGG  >  NC_002947/5728696‑5728987
                                                                                                                                                   |                                                                                                                                                
aCGAGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGaa                                                                                                                                                 >  3:77092/1‑149 (MQ=255)
     cAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAg                                                                                                                                            <  1:145729/149‑1 (MQ=255)
      aaCCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAgg                                                                                                                                           >  8:146159/1‑149 (MQ=255)
         ccTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTc                                                                                                                                         <  8:188549/148‑1 (MQ=255)
         ccTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTc                                                                                                                                         >  7:188549/1‑148 (MQ=255)
             gCCGAAAACAAATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATg                                                                                                                                     <  1:49708/148‑1 (MQ=255)
              ccGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCa                                                                                                                                   <  4:128238/149‑1 (MQ=255)
                   aaCAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAgcgcg                                                                                                                              >  3:342525/1‑149 (MQ=255)
                      aGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGa                                                                                                                           >  7:263786/1‑149 (MQ=255)
                           aTGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATc                                                                                                                      <  8:261831/149‑1 (MQ=255)
                               aaCCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGa                                                                                                                           >  6:130325/1‑140 (MQ=255)
                               aaCCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGa                                                                                                                           <  5:130325/140‑1 (MQ=255)
                               aaCCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCcag                                                                                                                   <  7:326649/148‑1 (MQ=255)
                               aaCCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCcag                                                                                                                   >  5:35665/1‑148 (MQ=255)
                                  cAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAgg                                                                                                               >  3:364884/1‑149 (MQ=255)
                                   aaCGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGCACCGGACCTACGAACGGAGGTCGATGCCGCGCGCGATTATCGAGCTGGt                                                                                                              >  4:284589/1‑149 (MQ=255)
                                      ggCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGc                                                                                                           <  3:191407/149‑1 (MQ=255)
                                       gCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCg                                                                                                          <  8:88683/149‑1 (MQ=255)
                                        ccAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGt                                                                                                         <  1:129048/149‑1 (MQ=255)
                                              aCAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAAc                                                                                                   <  2:69668/149‑1 (MQ=255)
                                                aaaTGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCGATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGc                                                                                                 <  6:298636/149‑1 (MQ=255)
                                                     ccGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCa                                                                                             <  4:364884/148‑1 (MQ=255)
                                                         gTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTg                                                                                        <  4:221896/149‑1 (MQ=255)
                                                          ttGGCGAGCACTGTTCGGACCCCGAGCGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTgg                                                                                       <  4:100941/149‑1 (MQ=255)
                                                           tGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGa                                                                                      <  7:146159/149‑1 (MQ=255)
                                                             gCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAg                                                                                     >  3:359013/1‑148 (MQ=255)
                                                             gCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAg                                                                                     >  2:77730/1‑148 (MQ=255)
                                                             gCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGc                                                                                    <  6:35665/149‑1 (MQ=255)
                                                                     tGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTAtt                                                                             >  6:91872/1‑148 (MQ=255)
                                                                         cGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATc                                                                         <  4:342525/148‑1 (MQ=255)
                                                                          ggACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCg                                                                        >  4:300734/1‑148 (MQ=255)
                                                                            aCCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTAc                                                                     <  3:276539/149‑1 (MQ=255)
                                                                                         gTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAgg                                                                                                               <  2:126831/94‑1 (MQ=255)
                                                                                         gTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAgg                                                                                                               >  1:126831/1‑94 (MQ=255)
                                                                                         gTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTg                                                          <  1:27728/147‑1 (MQ=255)
                                                                                         gTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTg                                                          >  2:27728/1‑147 (MQ=255)
                                                                                         gTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGtt                                                        >  8:323105/1‑149 (MQ=255)
                                                                                              gATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCt                                                   >  8:92174/1‑149 (MQ=255)
                                                                                                ttCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCtgt                                                 >  5:335377/1‑149 (MQ=255)
                                                                                                   cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaa                                              >  8:17742/1‑149 (MQ=255)
                                                                                                   cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaa                                              >  5:76265/1‑149 (MQ=255)
                                                                                                   cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGa                                               >  3:82764/1‑148 (MQ=255)
                                                                                                    ccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaac                                             <  4:157157/149‑1 (MQ=255)
                                                                                                     cAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCACGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaac                                             >  2:240971/1‑148 (MQ=255)
                                                                                                      aaaCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaacaa                                           >  6:306800/1‑149 (MQ=255)
                                                                                                        aCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACg                                         <  5:91872/149‑1 (MQ=255)
                                                                                                               cAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttt                                   <  7:17742/148‑1 (MQ=255)
                                                                                                               cAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGAGATTATCCCGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTATCCTGTGAACAAGGGGttttt                                  >  6:225363/1‑149 (MQ=255)
                                                                                                                aGACAATGCTCGAGTCAGAGTGAAACGTACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                 <  8:263786/149‑1 (MQ=255)
                                                                                                                aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                 <  4:359013/149‑1 (MQ=255)
                                                                                                                aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                 <  4:82764/149‑1 (MQ=255)
                                                                                                                aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCACGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                 <  1:240971/149‑1 (MQ=255)
                                                                                                                     aTGCTCGAGTCAGAGTGAAACTGACCTACGAACGCAGGTCCATGCATCGCTCGATTATCCAGCAGGATGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCt                            <  5:285237/149‑1 (MQ=255)
                                                                                                                        cTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCa                          <  3:300734/148‑1 (MQ=255)
                                                                                                                           gAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATAtt                      >  3:187583/1‑149 (MQ=255)
                                                                                                                           gAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATAtt                      >  3:192725/1‑149 (MQ=255)
                                                                                                                                agagTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                 >  7:34425/1‑133 (MQ=255)
                                                                                                                                agagTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                 <  8:34425/133‑1 (MQ=255)
                                                                                                                                     tgcatggACCTGCGTTCGTAGGTCCATGTAGCGCGCGATTATACAGAAGGTTGCGTGACAACGCATCAGGATGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAAAGGGTTTTTTGCCGTCATATTTGTAACATa             >  6:90204/6‑148 (MQ=255)
                                                                                                                                       aaCGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt          <  7:92174/149‑1 (MQ=255)
                                                                                                                                       aaCGGACCAACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGTTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt          >  7:138543/1‑149 (MQ=255)
                                                                                                                                        atggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt          >  2:203079/3‑148 (MQ=255)
                                                                                                                                        atggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt          <  1:203079/146‑1 (MQ=255)
                                                                                                                                        atggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATg         >  5:225094/3‑149 (MQ=255)
                                                                                                                                         tggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt          <  3:79865/146‑1 (MQ=255)
                                                                                                                                         tggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt          >  4:79865/2‑147 (MQ=255)
                                                                                                                                           gACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTa      >  5:284493/1‑149 (MQ=255)
                                                                                                                                             ccTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTAcc    <  3:48059/149‑1 (MQ=255)
                                                                                                                                               tACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCgg  >  1:49247/1‑149 (MQ=255)
                                                                                                                                                 cGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGATCAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCgg  <  1:77730/147‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
ACGAGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCGG  >  NC_002947/5728696‑5728987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑