Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,728,847 C→T 8.8% intergenic (‑210/+114) opgG ← / ← dtd glucans biosynthesis protein G/D‑tyrosyl‑tRNA(Tyr) deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,728,8470CT 8.8% 195.9 / 6.3 70intergenic (‑210/+114)opgG/dtdglucans biosynthesis protein G/D‑tyrosyl‑tRNA(Tyr) deacylase
Reads supporting (aligned to +/- strand):  ref base C (29/34);  new base T (4/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

CAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCGGGCCAT  >  NC_002947/5728701‑5728992
                                                                                                                                                  |                                                                                                                                                 
cAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAg                                                                                                                                                 <  1:145729/149‑1 (MQ=255)
 aaCCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAgg                                                                                                                                                >  8:146159/1‑149 (MQ=255)
    ccTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTc                                                                                                                                              >  7:188549/1‑148 (MQ=255)
    ccTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTc                                                                                                                                              <  8:188549/148‑1 (MQ=255)
        gCCGAAAACAAATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATg                                                                                                                                          <  1:49708/148‑1 (MQ=255)
         ccGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCa                                                                                                                                        <  4:128238/149‑1 (MQ=255)
              aaCAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAgcgcg                                                                                                                                   >  3:342525/1‑149 (MQ=255)
                 aGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGa                                                                                                                                >  7:263786/1‑149 (MQ=255)
                      aTGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATc                                                                                                                           <  8:261831/149‑1 (MQ=255)
                          aaCCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGa                                                                                                                                >  6:130325/1‑140 (MQ=255)
                          aaCCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGa                                                                                                                                <  5:130325/140‑1 (MQ=255)
                          aaCCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCcag                                                                                                                        <  7:326649/148‑1 (MQ=255)
                          aaCCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCcag                                                                                                                        >  5:35665/1‑148 (MQ=255)
                             cAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAgg                                                                                                                    >  3:364884/1‑149 (MQ=255)
                              aaCGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGCACCGGACCTACGAACGGAGGTCGATGCCGCGCGCGATTATCGAGCTGGt                                                                                                                   >  4:284589/1‑149 (MQ=255)
                                 ggCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGc                                                                                                                <  3:191407/149‑1 (MQ=255)
                                  gCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCg                                                                                                               <  8:88683/149‑1 (MQ=255)
                                   ccAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGt                                                                                                              <  1:129048/149‑1 (MQ=255)
                                         aCAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAAc                                                                                                        <  2:69668/149‑1 (MQ=255)
                                           aaaTGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCGATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGc                                                                                                      <  6:298636/149‑1 (MQ=255)
                                                ccGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCa                                                                                                  <  4:364884/148‑1 (MQ=255)
                                                    gTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTg                                                                                             <  4:221896/149‑1 (MQ=255)
                                                     ttGGCGAGCACTGTTCGGACCCCGAGCGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTgg                                                                                            <  4:100941/149‑1 (MQ=255)
                                                      tGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGa                                                                                           <  7:146159/149‑1 (MQ=255)
                                                        gCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAg                                                                                          >  3:359013/1‑148 (MQ=255)
                                                        gCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAg                                                                                          >  2:77730/1‑148 (MQ=255)
                                                        gCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGc                                                                                         <  6:35665/149‑1 (MQ=255)
                                                                tGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTAtt                                                                                  >  6:91872/1‑148 (MQ=255)
                                                                    cGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATc                                                                              <  4:342525/148‑1 (MQ=255)
                                                                     ggACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCg                                                                             >  4:300734/1‑148 (MQ=255)
                                                                       aCCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTAc                                                                          <  3:276539/149‑1 (MQ=255)
                                                                                    gTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAgg                                                                                                                    <  2:126831/94‑1 (MQ=255)
                                                                                    gTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAgg                                                                                                                    >  1:126831/1‑94 (MQ=255)
                                                                                    gTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTg                                                               <  1:27728/147‑1 (MQ=255)
                                                                                    gTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTg                                                               >  2:27728/1‑147 (MQ=255)
                                                                                    gTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGtt                                                             >  8:323105/1‑149 (MQ=255)
                                                                                         gATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCt                                                        >  8:92174/1‑149 (MQ=255)
                                                                                           ttCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCtgt                                                      >  5:335377/1‑149 (MQ=255)
                                                                                              cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaa                                                   >  8:17742/1‑149 (MQ=255)
                                                                                              cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaa                                                   >  5:76265/1‑149 (MQ=255)
                                                                                              cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGa                                                    >  3:82764/1‑148 (MQ=255)
                                                                                               ccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaac                                                  <  4:157157/149‑1 (MQ=255)
                                                                                                cAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCACGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaac                                                  >  2:240971/1‑148 (MQ=255)
                                                                                                 aaaCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaacaa                                                >  6:306800/1‑149 (MQ=255)
                                                                                                   aCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACg                                              <  5:91872/149‑1 (MQ=255)
                                                                                                          cAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttt                                        <  7:17742/148‑1 (MQ=255)
                                                                                                          cAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGAGATTATCCCGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTATCCTGTGAACAAGGGGttttt                                       >  6:225363/1‑149 (MQ=255)
                                                                                                           aGACAATGCTCGAGTCAGAGTGAAACGTACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                      <  8:263786/149‑1 (MQ=255)
                                                                                                           aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                      <  4:359013/149‑1 (MQ=255)
                                                                                                           aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                      <  4:82764/149‑1 (MQ=255)
                                                                                                           aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCACGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                      <  1:240971/149‑1 (MQ=255)
                                                                                                                aTGCTCGAGTCAGAGTGAAACTGACCTACGAACGCAGGTCCATGCATCGCTCGATTATCCAGCAGGATGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCt                                 <  5:285237/149‑1 (MQ=255)
                                                                                                                   cTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCa                               <  3:300734/148‑1 (MQ=255)
                                                                                                                      gAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATAtt                           >  3:192725/1‑149 (MQ=255)
                                                                                                                      gAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATAtt                           >  3:187583/1‑149 (MQ=255)
                                                                                                                           agagTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                      <  8:34425/133‑1 (MQ=255)
                                                                                                                           agagTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                                      >  7:34425/1‑133 (MQ=255)
                                                                                                                                tgcatggACCTGCGTTCGTAGGTCCATGTAGCGCGCGATTATACAGAAGGTTGCGTGACAACGCATCAGGATGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAAAGGGTTTTTTGCCGTCATATTTGTAACATa                  >  6:90204/6‑148 (MQ=255)
                                                                                                                                  aaCGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt               <  7:92174/149‑1 (MQ=255)
                                                                                                                                  aaCGGACCAACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGTTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt               >  7:138543/1‑149 (MQ=255)
                                                                                                                                   atggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt               >  2:203079/3‑148 (MQ=255)
                                                                                                                                   atggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt               <  1:203079/146‑1 (MQ=255)
                                                                                                                                   atggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATg              >  5:225094/3‑149 (MQ=255)
                                                                                                                                    tggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt               <  3:79865/146‑1 (MQ=255)
                                                                                                                                    tggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt               >  4:79865/2‑147 (MQ=255)
                                                                                                                                      gACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTa           >  5:284493/1‑149 (MQ=255)
                                                                                                                                        ccTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTAcc         <  3:48059/149‑1 (MQ=255)
                                                                                                                                          tACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCgg       >  1:49247/1‑149 (MQ=255)
                                                                                                                                            cGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGATCAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCgg       <  1:77730/147‑1 (MQ=255)
                                                                                                                                                cGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGGTTTTTGACCTCATATTTGTAACAAAAATGTTACCGGGCcat  >  2:326141/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCGGGCCAT  >  NC_002947/5728701‑5728992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑