Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,747,318 A→G 6.2% L219P (CTG→CCG)  PP_5043 ← PhoPQ‑activated pathogenicity‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,747,3180AG 6.2% 185.8 / 2.2 64L219P (CTG→CCG) PP_5043PhoPQ‑activated pathogenicity‑like protein
Reads supporting (aligned to +/- strand):  ref base A (27/33);  new base G (2/2);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

CTCCGCAGCCTGATAGGACCAAAGCGCCAATGGCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGC  >  NC_002947/5747176‑5747460
                                                                                                                                              |                                                                                                                                               
cTCCGCAGCCTGATAGGACCAAAGCGCCAATGGCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATg                                                                                                                                           <  7:264498/149‑1 (MQ=255)
  ccGCAGCCTGATAGGACCAAAGCGCCAATGGCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCa                                                                                                                                         >  1:11362/1‑149 (MQ=255)
   cGCAGCCTGATAGGACCAAAGCGCCAATGGCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCTGACGGCCATggc                                                                                                                                         <  8:17128/148‑3 (MQ=255)
                gACCAAAGCGCCAATGGCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGttt                                                                                                                          >  1:179835/1‑149 (MQ=255)
                 aCCAAAGCGCCAATGGCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTc                                                                                                                         >  3:285278/1‑149 (MQ=255)
                 aCCAAAGCGCCAATGGCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGGTTc                                                                                                                         >  7:291500/1‑149 (MQ=255)
                   cAAAGCGCCAATGGCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGa                                                                                                                       <  2:294584/149‑1 (MQ=255)
                    aaaGCGCCAATGGCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAg                                                                                                                      <  3:117244/149‑1 (MQ=255)
                    aaaGCGCCAATGGCCATGAACCACCGAACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAg                                                                                                                      >  1:9723/1‑149 (MQ=255)
                     aaGCGCCAATGGCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAgg                                                                                                                     <  5:68979/149‑1 (MQ=255)
                        cgcCAATGGCCATGATCCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGGCGGCAACATCGATGACGCTGCGCACGATGTCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCAc                                                                                                                  <  5:271038/149‑1 (MQ=255)
                          ccAATGGCCATTAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCAATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCg                                                                                                                <  6:8453/149‑1 (MQ=255)
                          ccAATGGCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCg                                                                                                                <  7:165038/149‑1 (MQ=255)
                            aaTGGCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGt                                                                                                              >  6:333755/1‑149 (MQ=255)
                               ggCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGACAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGtgat                                                                                                           >  7:225842/1‑149 (MQ=255)
                                 ccATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGtgatga                                                                                                         <  5:333755/149‑1 (MQ=255)
                                  cATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGtgatgat                                                                                                        <  2:11362/149‑1 (MQ=255)
                                     gAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGcc                                                                                                                                              >  7:261803/1‑109 (MQ=255)
                                     gAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGcc                                                                                                                                              <  8:261803/109‑1 (MQ=255)
                                     gAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCAGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGtgatgatga                                                                                                      >  3:188544/1‑148 (MQ=255)
                                       accaccGTACCGCTTTCGCAAGCCCTCCAGCATTCTGGAAGTGTCGGCAACATCGATGACGCTGGTCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAAc                                                                                                    <  3:229862/148‑1 (MQ=255)
                                       accaccGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACt                                                                                                   >  8:46810/1‑149 (MQ=255)
                                         caccGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGc                                                                                                 <  4:92177/149‑1 (MQ=255)
                                           ccGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTc                                                                                               <  7:296750/149‑1 (MQ=255)
                                             gTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTc                                                                                               >  5:39526/1‑147 (MQ=255)
                                              tACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAg                                                                                            <  8:291500/149‑1 (MQ=255)
                                              tACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAg                                                                                            <  2:179835/149‑1 (MQ=255)
                                              tACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAg                                                                                            <  4:285278/149‑1 (MQ=255)
                                                    ttGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAg                                                                                            <  4:219000/143‑1 (MQ=255)
                                                    ttGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAg                                                                                            >  3:219000/1‑143 (MQ=255)
                                                        gcAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCg                                                                                  <  3:146949/149‑1 (MQ=255)
                                                        gcAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAAATACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAACCCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTc                                                                                   <  1:260978/149‑1 (MQ=255)
                                                            cacttCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGc                                                                               >  2:301738/5‑148 (MQ=255)
                                                               cTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAAcg                                                                           >  5:104206/1‑149 (MQ=255)
                                                                    gCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTACTCGAGGCGTAGCTCGGGCAACGCGTCa                                                                      <  3:351440/149‑1 (MQ=255)
                                                                            gAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAg                                                              <  8:174687/149‑1 (MQ=255)
                                                                                    ggCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGc                                                      >  8:96308/1‑149 (MQ=255)
                                                                                       aaCATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGcccc                                                   >  4:295515/1‑149 (MQ=255)
                                                                                         cATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCctg                                                 >  1:114157/1‑149 (MQ=255)
                                                                                           tCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCctgct                                               <  7:46810/149‑1 (MQ=255)
                                                                                             gATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTgg                                             >  2:224637/1‑149 (MQ=255)
                                                                                              aTGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCGATGCCCCTGCTGGc                                            <  7:96308/149‑1 (MQ=255)
                                                                                                        gCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGc                                   >  3:96021/1‑148 (MQ=255)
                                                                                                          aCGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCAt                                >  4:228732/1‑149 (MQ=255)
                                                                                                          aCGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCAt                                >  5:311190/1‑149 (MQ=255)
                                                                                                          aCGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCAt                                <  6:311190/149‑1 (MQ=255)
                                                                                                           cGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCAtt                               <  2:299344/149‑1 (MQ=255)
                                                                                                           cGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCAtt                               <  4:188544/149‑1 (MQ=255)
                                                                                                            gATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTg                              <  4:96021/149‑1 (MQ=255)
                                                                                                               ggCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGca                           <  6:104206/149‑1 (MQ=255)
                                                                                                                      aCCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCa                     <  1:224637/148‑1 (MQ=255)
                                                                                                                       cccGGTCATCGGCCATGGCCGTCAGCCATGGACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGc                   >  1:31421/1‑149 (MQ=255)
                                                                                                                        ccGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCg                  >  3:261273/1‑149 (MQ=255)
                                                                                                                          ggTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCgg                 >  3:147976/1‑148 (MQ=255)
                                                                                                                          ggTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCgg                 >  5:111501/1‑148 (MQ=255)
                                                                                                                           gTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCa               <  6:39526/149‑1 (MQ=255)
                                                                                                                                  tgcATGGCTGACGGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCgg        >  7:209826/3‑149 (MQ=255)
                                                                                                                                   gcATGGCTGACGGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCct                                                  >  7:93174/2‑106 (MQ=255)
                                                                                                                                   gcATGGCTGACGGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCct                                                  <  8:93174/105‑1 (MQ=255)
                                                                                                                                    cATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCgg                 <  2:354943/138‑1 (MQ=255)
                                                                                                                                    cATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCgg                 >  1:354943/1‑138 (MQ=255)
                                                                                                                                    cATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGgcg      >  5:174793/1‑149 (MQ=255)
                                                                                                                                     aTGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGgcgc     <  6:294789/149‑1 (MQ=255)
                                                                                                                                        gCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGc  <  6:111501/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CTCCGCAGCCTGATAGGACCAAAGCGCCAATGGCCATGAACCACCGTACCGCTTGCGCAAGCCCTCCAGCATGCTGGAAGTGTCGGCAACATCGATGACGCTGGGCACGATGGCAATTACCCGGTCATCGGCCATGGCCGTCAGCCATGCACTCCAA‑CCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGC  >  NC_002947/5747176‑5747460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑