Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,790,143 A→T 12.1% intergenic (+22/+46) ynfM → / ← PP_5073 efflux transporter/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,790,1430AT12.1% 143.0 / 11.7 58intergenic (+22/+46)ynfM/PP_5073efflux transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (24/27);  new base T (4/3);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

CGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTG  >  NC_002947/5790000‑5790289
                                                                                                                                               |                                                                                                                                                  
cgggcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCca                                                                                                                                                >  1:234927/1‑148 (MQ=255)
 gggcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCTCCca                                                                                                                                                >  3:193666/1‑147 (MQ=255)
   gcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAAc                                                                                                                                            <  3:86858/149‑1 (MQ=255)
        gtgtTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCtggg                                                                                                                                       <  7:215339/149‑3 (MQ=255)
         tgtTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTgg                                                                                                                                      <  2:150350/149‑1 (MQ=255)
                gCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCTCCCACAGGTTGTGGGTGCgg                                                                                                                                <  4:72490/148‑1 (MQ=255)
                gCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCTCCCACAGGTTGTGGGTGCg                                                                                                                                 <  4:193666/147‑1 (MQ=255)
                gCACCAGGGGGGCTGGAACGGGATCGGGCGGGTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCg                                                                                                                                 <  8:78483/147‑1 (MQ=255)
                 taCCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGgt                                                                                                                              <  3:7327/148‑1 (MQ=255)
                 cACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCTAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGgt                                                                                                                              <  5:253830/149‑1 (MQ=255)
                 cACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGgt                                                                                                                              <  6:302610/149‑1 (MQ=255)
                    cAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGgtgt                                                                                                                            >  7:204251/1‑148 (MQ=255)
                     aGTGGGGCTGGAACGGGATCGGGGTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTTCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGa                                                                                                                          <  6:53419/149‑1 (MQ=255)
                     aGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGa                                                                                                                          <  5:348620/149‑1 (MQ=255)
                         gggCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCa                                                                                                                       <  5:373687/148‑1 (MQ=255)
                                  cGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGc                                                                                                              >  6:20971/1‑148 (MQ=255)
                                      aTCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCat                                                                                                           >  8:88585/1‑147 (MQ=255)
                                             tGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACGTGAGCAAGTTCCCACCCAAACCGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGt                                                                                                  <  5:270864/149‑1 (MQ=255)
                                              gTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGtt                                                                                                 >  3:59409/1‑149 (MQ=255)
                                               ttCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGt                                                                                                  <  2:234927/147‑1 (MQ=255)
                                                 cATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCTCCCACAGGTTGTGGGTGCgg                                                                                                                                >  3:45598/1‑115 (MQ=38)
                                                 cATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCTCCCACAGGTTGTGGGTGCgg                                                                                                                                <  4:45598/115‑1 (MQ=38)
                                                  aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTtgat                                                                                              >  3:97822/1‑148 (MQ=255)
                                                      gCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACACACAACCTGTGGGAGCGGGTGTGACCACCAAGAGGCCATCAGATCAGTTGATGAtct                                                                                         >  7:284929/1‑149 (MQ=255)
                                                                   ggTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatccac                                                                            <  3:326854/149‑1 (MQ=255)
                                                                        ccTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatccacatcca                                                                       <  4:97822/149‑1 (MQ=255)
                                                                        ccTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatcaacatcc                                                                        >  4:147067/1‑148 (MQ=255)
                                                                                     gTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCtcat                                                          >  1:348688/1‑149 (MQ=255)
                                                                                        gCACCTGAGCAAGTTGCCACCCAAAACGGGGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGTGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCtcatca                                                        <  4:186989/148‑1 (MQ=255)
                                                                                           ccTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTg                                                    >  4:235069/1‑149 (MQ=255)
                                                                                            cTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCg                                                                                                                                 <  2:227203/71‑1 (MQ=255)
                                                                                            cTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCg                                                                                                                                 >  1:227203/1‑71 (MQ=255)
                                                                                                    gctGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAAc                                           <  1:117938/147‑1 (MQ=255)
                                                                                                         cACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGAtc                                                                                          >  7:28279/1‑97 (MQ=255)
                                                                                                         cACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGAtc                                                                                          <  8:28279/97‑1 (MQ=255)
                                                                                                         cACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCAGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCAc                                      >  4:258164/1‑149 (MQ=255)
                                                                                                          aCCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCAcc                                     >  3:195484/1‑149 (MQ=255)
                                                                                                            ccAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGAGAATATACACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCgg                                   >  4:334708/1‑149 (MQ=255)
                                                                                                              aaaaCGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTCATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGt                                  >  8:202347/1‑148 (MQ=255)
                                                                                                                     tgtgATTCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCa                                       <  8:312095/136‑1 (MQ=255)
                                                                                                                     tgtgATACATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCa                                       >  7:312095/1‑136 (MQ=255)
                                                                                                                       tgATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGTGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCAcc                        <  5:20971/149‑1 (MQ=255)
                                                                                                                          tGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTg                     <  7:88585/149‑1 (MQ=255)
                                                                                                                           gCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTgg                    >  2:246853/1‑149 (MQ=255)
                                                                                                                             aTTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCt                             >  2:331361/1‑138 (MQ=255)
                                                                                                                             aTTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCt                             <  1:331361/138‑1 (MQ=255)
                                                                                                                                cGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCtt                <  2:348688/148‑1 (MQ=255)
                                                                                                                                 gTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGt              <  4:195484/149‑1 (MQ=255)
                                                                                                                                  tGGTCACACCCGCTCCCACAGGTTGTGGGTGCGGGCGTGCCCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTc             >  7:227318/1‑149 (MQ=37)
                                                                                                                                   gggCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTCGACTTGTCg            >  3:218018/1‑149 (MQ=255)
                                                                                                                                     gCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGt           <  3:147067/148‑1 (MQ=255)
                                                                                                                                          cccGCTCCCACAGGTTGTGGGTGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACg     >  6:376546/1‑149 (MQ=255)
                                                                                                                                           ccGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCttt                                                <  2:172597/105‑1 (MQ=255)
                                                                                                                                           ccGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCttt                                                >  1:172597/1‑105 (MQ=255)
                                                                                                                                           ccGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTGTTGTCGTTGAACgg    >  3:116213/1‑149 (MQ=255)
                                                                                                                                            cGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGgt   <  3:258164/149‑1 (MQ=255)
                                                                                                                                             gCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCTTAAGGTGCTTTTCAACCTCACCGTTCAGCTTCACCTTGGTCTTGTCGTTGAAAGgtg  >  1:273802/1‑149 (MQ=255)
                                                                                                                                             gCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCttt                                                >  2:346716/1‑103 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGCACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTG  >  NC_002947/5790000‑5790289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑