Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,849,048	A→G	9.6%	intergenic (+23/‑248)	rlmI → / → ilvD	ribosomal RNA large subunit methyltransferase I/dihydroxy‑acid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,849,048	0	A	G	9.6%	170.6 / 6.8	62	intergenic (+23/‑248)	rlmI/ilvD	ribosomal RNA large subunit methyltransferase I/dihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base A (30/26);  new base G (4/2);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTGGACCGCAACATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATCCCGCGCGG  >  NC_002947/5848901‑5849194                                                                                                                                                     |                                                                                                                                                   aCCTGGACCGCAACATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAg                                                                                                                                                   >  2:36962/1‑149 (MQ=255)  ccTGGACCGCAACATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGc                                                                                                                                                  >  7:268258/1‑149 (MQ=255)  ccTGGACCGCAACATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGc                                                                                                                                                  >  3:103947/1‑149 (MQ=255)      gACCGCAACATGCGGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCTGGGCGGcc                                                                                                                                              >  1:348205/1‑149 (MQ=255)            aaCATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCTGGGCGGCCCttttt                                                                                                                                        >  7:210297/1‑147 (MQ=255)            aaCATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCTGGGCGGCCCttttt                                                                                                                                        <  8:210297/149‑3 (MQ=255)              cATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTg                                                                                                                                      <  8:268258/149‑1 (MQ=255)                tGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACAGCCGGCCATCGCCGA‑AACCCTCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGcc                                                                                                                                    <  6:99503/149‑1 (MQ=255)                         tCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATcccc                                                                                                                           >  3:166976/1‑149 (MQ=255)                         tCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATcccc                                                                                                                           >  4:181428/1‑149 (MQ=255)                             gcgagGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGc                                                                                                                       <  1:36962/149‑1 (MQ=255)                               gagGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATccccc                                                                                                                          <  8:284847/144‑1 (MQ=255)                               gagGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATccccc                                                                                                                          >  7:284847/1‑144 (MQ=255)                                agGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCaa                                                                                                                    <  5:344144/149‑1 (MQ=255)                                      tCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCCATCCCCCCGCCAACCATc                                                                                                               >  5:120163/1‑148 (MQ=255)                                          ggCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCcaca                                                                                                           >  4:98893/1‑148 (MQ=255)                                            cccGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCt                                                                                                        <  3:98893/149‑1 (MQ=255)                                             ccGGATCACCCGGTACACCCGGCCATCGCCGATAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCt                                                                                                        >  1:44013/1‑149 (MQ=255)                                                 aTCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCAtt                                                                                                   >  5:202273/1‑149 (MQ=255)                                                    aCCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTcc                                                                                                 <  5:162791/148‑1 (MQ=255)                                                     cccGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCt                                                                                               >  3:147498/1‑149 (MQ=255)                                                       ctgTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTc                                                                                                                                         <  3:221679/103‑1 (MQ=255)                                                       cGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTc                                                                                                                                         >  4:221679/1‑105 (MQ=255)                                                                  gCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCg                                                                                  <  4:103947/149‑1 (MQ=255)                                                                     aTCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGgtg                                                                               >  5:200817/1‑149 (MQ=255)                                                                      tCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGgtgt                                                                              <  6:200817/149‑1 (MQ=255)                                                                        gCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTa                                                                             <  6:202273/148‑1 (MQ=255)                                                                               aCCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCgg                                                                      <  3:181428/149‑1 (MQ=255)                                                                                cccGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGc                                                                     <  6:136930/149‑1 (MQ=255)                                                                                cccGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGc                                                                     <  1:41931/149‑1 (MQ=255)                                                                                  cGCTACATCAAAAGCATCACCTGCCGTTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGGATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCt                                                                   <  4:132556/149‑1 (MQ=255)                                                                                      aCATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTAtt                                                                >  8:334941/1‑148 (MQ=255)                                                                                        aTCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCAt                                                             >  2:365307/1‑149 (MQ=255)                                                                                          cAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCg                                                           >  5:26828/1‑149 (MQ=255)                                                                                                  tCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCAt                                                   >  6:45996/1‑149 (MQ=255)                                                                                                       tGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTc                                                     <  5:163317/142‑1 (MQ=255)                                                                                                       tGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTc                                                     >  6:163317/1‑142 (MQ=255)                                                                                                             ttACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGt                                        <  4:166976/149‑1 (MQ=255)                                                                                                                 tGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGtt                                       >  7:325149/1‑146 (MQ=255)                                                                                                                 tGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGtt                                       <  8:325149/146‑1 (MQ=255)                                                                                                                   ccAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTAt                                    <  2:44013/147‑1 (MQ=255)                                                                                                                   ccAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTAt                                    <  1:365307/147‑1 (MQ=255)                                                                                                                   ccAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTAt                                    >  5:153449/1‑147 (MQ=255)                                                                                                                   ccAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGa                                  <  7:334941/149‑1 (MQ=255)                                                                                                                    cAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAg                                 >  5:24930/1‑149 (MQ=255)                                                                                                                       aCAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGctc                              >  3:31921/1‑149 (MQ=255)                                                                                                                           cTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGctct                             <  8:135396/146‑1 (MQ=255)                                                                                                                           cTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGctct                             >  7:135396/1‑146 (MQ=255)                                                                                                                            ttccgtCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTgg                           <  7:8945/142‑1 (MQ=255)                                                                                                                                   tGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCAcg                  >  3:181016/1‑149 (MQ=255)                                                                                                                                         aaaGGGCCGCTGGGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATGTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCTTCCCCGGCGGGTTTATGa                                  <  5:216320/127‑1 (MQ=255)                                                                                                                                         aaaGGGCCGCTGGGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCACTCAACCCCGGCGGGGTTATGa                                  >  6:216320/1‑127 (MQ=255)                                                                                                                                            gggCCGCTGGGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATcc         >  7:283935/1‑149 (MQ=255)                                                                                                                                              gccgccCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATCCcg       >  4:241570/1‑149 (MQ=255)                                                                                                                                                cgccCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATccc        <  5:161435/146‑1 (MQ=255)                                                                                                                                                cgccCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATccc        >  6:161435/1‑146 (MQ=255)                                                                                                                                                 gccCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCcggc                                            >  3:17879/1‑109 (MQ=255)                                                                                                                                                 gccCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCcggc                                            <  4:17879/109‑1 (MQ=255)                                                                                                                                                 gccCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATCCcgcgc    <  4:147498/149‑1 (MQ=255)                                                                                                                                                 gccCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATCCcgcgc    >  4:347696/1‑149 (MQ=255)                                                                                                                                                   ccAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATCCCGCGCgg  >  2:100143/1‑149 (MQ=255)                                                                                                                                                   ccAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATCCCGCGCgg  >  5:50413/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACCTGGACCGCAACATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGA‑AACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATCCCGCGCGG  >  NC_002947/5848901‑5849194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑