Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,874,820 A→C 6.2% S451A (TCG→GCG) ‡ ilvA‑II ← threonine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,874,8200AC 6.2% 253.8 / 7.2 81S451A (TCG→GCG) ‡ilvA‑IIthreonine deaminase
Reads supporting (aligned to +/- strand):  ref base A (37/39);  new base C (2/3);  total (39/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01

TAGGCCGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTGGCCAATTCGTT  >  NC_002947/5874675‑5874967
                                                                                                                                                 |                                                                                                                                                   
tAGGCCGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCgaga                                                                                                                                                   <  7:123434/148‑1 (MQ=255)
tAGGCCGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGAt                                                                                                                                                  >  3:344141/1‑149 (MQ=255)
   gCCGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGt                                                                                                                                                >  1:132891/1‑148 (MQ=255)
   gCCGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCACCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGtt                                                                                                                                               >  4:160319/1‑149 (MQ=255)
    ccGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCTCGATGTTc                                                                                                                                              <  3:361656/149‑1 (MQ=255)
    ccGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTc                                                                                                                                              <  6:191924/149‑1 (MQ=255)
     cGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTcc                                                                                                                                             <  6:130525/149‑1 (MQ=255)
       ggTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCTCGATGTTCCac                                                                                                                                           <  5:98646/149‑2 (MQ=255)
       ggTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAg                                                                                                                                           >  7:167377/1‑149 (MQ=255)
           ttCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAgcgc                                                                                                                                        <  5:126839/148‑1 (MQ=255)
               gTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGcgcccgcc                                                                                                                                   >  5:107671/1‑149 (MQ=255)
                  tcGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAg                                                                                                                                >  3:199043/1‑149 (MQ=255)
                    gTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCt                                                                                                                              <  2:94765/149‑1 (MQ=255)
                    gTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACAAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGAAGTGGAACATCGAGAAGTTCCAGCGCCCGCCCAGca                                                                                                                              >  7:151653/1‑148 (MQ=255)
                     tCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCtt                                                                                                                             >  7:251378/1‑149 (MQ=255)
                       ccAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCttgt                                                                                                                           >  7:498/1‑149 (MQ=255)
                       ccAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCttgt                                                                                                                           >  3:97145/1‑149 (MQ=255)
                        cAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCttgtt                                                                                                                          <  8:167377/149‑1 (MQ=255)
                         aGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCttgtt                                                                                                                          >  7:18991/1‑148 (MQ=255)
                         aGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCgtgttg                                                                                                                         >  8:93097/1‑149 (MQ=255)
                            aCGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAg                                                                                                                       >  6:266926/1‑148 (MQ=255)
                                gTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGt                                                                                                                  <  6:294193/149‑1 (MQ=255)
                                 tAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGtt                                                                                                                 <  4:97145/149‑1 (MQ=255)
                                   aCCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTg                                                                                                                >  7:114084/1‑148 (MQ=255)
                                        tCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGt                                                                                                          >  7:204603/1‑149 (MQ=255)
                                        tCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGt                                                                                                          >  5:57272/1‑149 (MQ=255)
                                          ttGGCCAGCGCTGCCTGCACCAGGTGAGGTTCGTCGTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTg                                                                                                         <  4:344141/148‑1 (MQ=255)
                                            ggCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAAc                                                                                                             <  3:10878/142‑1 (MQ=255)
                                            ggCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACa                                                                                                            <  2:312762/143‑1 (MQ=255)
                                            ggCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGt                                                                                                          <  2:181128/145‑1 (MQ=255)
                                             gCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGcccc                                                                                                     >  5:323323/1‑149 (MQ=255)
                                                        cGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCgg                                                                                          <  8:204603/149‑1 (MQ=255)
                                                         ggCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCggg                                                                                         <  5:167192/149‑1 (MQ=255)
                                                         ggCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCggg                                                                                         <  8:114084/149‑1 (MQ=255)
                                                            aCCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGaa                                                                                      <  5:10127/149‑1 (MQ=255)
                                                            aCCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGaa                                                                                      <  1:135037/149‑1 (MQ=255)
                                                             ccAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAAt                                                                                     <  4:199043/149‑1 (MQ=255)
                                                                ggTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGAGCACGTCAGCCGCGAAATGGTTGCGGTAGTGGAAAATCGAGATGTTCCAGAGCACGCCCAGCTTGTTGATGAAGTTGAACAGTGc                                                                                                        >  3:190725/1‑127 (MQ=255)
                                                                ggTGACGTTCGTCCTCCGGCACCTGCAGACCCGCAACCACGCTCCCTTCAGCCGCGCCATTTTTGCGGTAGTGGAACATCTAGATGTTGAAGCGCCCGCCCAGATTGTTGAGGAAGTTGAACAGTGc                                                                                                        <  4:190725/127‑1 (MQ=255)
                                                                  tGACGTTCGTCCTCCGGGACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGaa                                                                                <  4:348146/149‑1 (MQ=255)
                                                                  tGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGaa                                                                                <  8:82131/149‑1 (MQ=255)
                                                                   gACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAg                                                                               <  8:18991/149‑1 (MQ=255)
                                                                      gTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGCACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAgcgc                                                                            >  5:257166/1‑149 (MQ=255)
                                                                      gTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAgcg                                                                             >  5:240640/1‑148 (MQ=255)
                                                                      gTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAgcg                                                                             >  2:207096/1‑148 (MQ=255)
                                                                          gTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCgcagca                                                                        >  5:176070/1‑149 (MQ=255)
                                                                          gTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCgcagc                                                                         <  6:266692/148‑1 (MQ=255)
                                                                             ctccGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCa                                                                     >  2:126506/1‑149 (MQ=255)
                                                                                cGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCAttt                                                                  >  7:20946/1‑149 (MQ=255)
                                                                                 ggCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTtc                                                                 <  6:19498/149‑1 (MQ=255)
                                                                                    aCCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTtcgtc                                                              <  6:57272/149‑1 (MQ=255)
                                                                                      cTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGc                                                            >  3:81244/1‑149 (MQ=255)
                                                                                       tGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCt                                                           >  8:321334/1‑149 (MQ=255)
                                                                                         cAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTgg                                                         <  5:266926/149‑1 (MQ=255)
                                                                                         cAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTgg                                                         <  6:107671/149‑1 (MQ=255)
                                                                                                gCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGc                                                  >  1:293784/1‑149 (MQ=255)
                                                                                                      acGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTg                                            >  2:70079/1‑149 (MQ=255)
                                                                                                        gcgcCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGgc                                          <  8:251378/149‑1 (MQ=255)
                                                                                                          gcCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTg                                            >  2:123214/1‑145 (MQ=255)
                                                                                                          gcCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTg                                            <  1:123214/145‑1 (MQ=255)
                                                                                                           cccGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGgccgc                                       <  2:132891/149‑1 (MQ=255)
                                                                                                            ccGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGc                                                                                                 >  4:127524/1‑90 (MQ=255)
                                                                                                            ccGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGc                                                                                                 <  3:127524/90‑1 (MQ=255)
                                                                                                                  gCCGCGCCATGGTTGCGGTACTGCAACATCGAGATGTTCAAGCGCCCGCCCAGCTTGATGAGGAAGTTGAACAGTGCACCTGGCCGCTCCGGGAATTCGAAGCGCAGCACCATTTCGTAGATGGCACCGGCCGAGTGGCCGCCGACCa                                 >  8:324766/1‑148 (MQ=255)
                                                                                                                    cgcgCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCatat                              >  7:169302/1‑149 (MQ=255)
                                                                                                                     gcgcCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCatat                              >  7:158417/1‑148 (MQ=255)
                                                                                                                          aTGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGaa                        <  8:498/149‑1 (MQ=255)
                                                                                                                             gTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAatat                     <  8:20946/149‑1 (MQ=255)
                                                                                                                              ttGCGGTAGTGTAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATg                    <  1:126506/149‑1 (MQ=255)
                                                                                                                              ttGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATg                    <  4:81244/149‑1 (MQ=255)
                                                                                                                                gCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGc                   <  6:176070/148‑1 (MQ=255)
                                                                                                                                gCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGc                   <  2:293784/148‑1 (MQ=255)
                                                                                                                                  ggTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGc                >  6:111729/1‑149 (MQ=255)
                                                                                                                                   gTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCt               >  1:245823/1‑149 (MQ=255)
                                                                                                                                     gctGGAACATCTCGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGATGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGAAGAATATGCAGCTTg             >  1:331014/3‑149 (MQ=255)
                                                                                                                                      gTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTg             <  1:70079/148‑1 (MQ=255)
                                                                                                                                      gTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTg             <  6:240640/148‑1 (MQ=255)
                                                                                                                                      ctGGAACATCTCGATGTTCCAGCGCCCGCCCAGCTTATTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAat                       <  8:12235/137‑1 (MQ=255)
                                                                                                                                      ctGGAACATCTCGATGTTCCAGCGCCCGCCCAGCTTATTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAat                       >  7:12235/2‑138 (MQ=255)
                                                                                                                                         gAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTGGcc          >  1:138462/1‑148 (MQ=255)
                                                                                                                                              tCGAGATGTTCCTGCGCCCGCCCAGCTTGTTGATGAAGTTGTACACTGGCCCTGGCCGCTCTGGGAATTCGAAGCGCAGCACCATTTCGTGGCTGGCAGCGGCCGAGTGGGGGCCGAGCATATGACGATTATGCAGCTTGGACATTTCg    <  8:151653/149‑1 (MQ=255)
                                                                                                                                              tCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTGGCCAATTCg    >  5:54422/1‑149 (MQ=255)
                                                                                                                                                gagaTGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTGGCCAATTCGtt  <  7:321334/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TAGGCCGGGTTTTCGGTCTCGTCCCAGTACGGGTAACCGATCTTGGCCAGCGCTGCCGGCACCAGGTGACGTTCGTCCTCCGGCACCTGCAGCCCCGCCACCACGCGCCCGTCAGCCGCGCCATGGTTGCGGTAGTGGAACATCGAGATGTTCCAGCGCCCGCCCAGCTTGTTGAGGAAGTTGAACAGTGCCCCTGGCCGCTCGGGGAATTCGAAGCGCAGCACCATTTCGTCGCTGGCACCGGCCGAGTGGCCGCCGACCATATGACGAATATGCAGCTTGGCCAATTCGTT  >  NC_002947/5874675‑5874967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑