| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 435,321 | A→C | 6.5% | intergenic (‑122/‑328) | glcB ← / → PP_0357 | malate synthase G/ACT domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 435,321 | 0 | A | C | 6.5% | 321.0 / 8.4 | 106 | intergenic (‑122/‑328) | glcB/PP_0357 | malate synthase G/ACT domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (54/45); new base C (4/3); total (58/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
CCACCGACTTGAACGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGT > NC_002947/435174‑435469 | ccACCGACTTGAACGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGAc < 6:267124/149‑1 (MQ=255)ccACCGACTTGAACGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGACCTGCCAGAc < 5:233651/149‑1 (MQ=255) aCCGACTTGAACGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAg > 7:272001/1‑149 (MQ=255) ccGACATGACCCTATCCAGTCATTGCTTGCCTCAATCTGCTCAGCTATTTCGCGCATCTCTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGAACCAGTCCATGCCCCCGGGGATGCTGGCGGGCCGCTGGGTGCGGCCTGCCAGACAgg > 6:97661/1‑149 (MQ=255) aCTTGAACGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTg > 6:234299/1‑149 (MQ=255) ttGAACGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGc > 5:152528/1‑149 (MQ=255) gAACGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCCGCCAGCCTgtctg > 5:230204/1‑145 (MQ=255) gAACGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCgg > 2:192348/1‑149 (MQ=255) aTCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCCGCCAGCCTGTCTGGCAgg > 6:210963/1‑147 (MQ=255) tCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCCGCCAGCCTGTCTGGCAggc < 5:210963/148‑3 (MQ=255) tCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGCCTGGCGGGCAg‑t > 3:219099/1‑147 (MQ=255) ccAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑tgtg > 1:159920/1‑149 (MQ=255) cAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCCGCCAGCCTGTCTGGCAggccgc < 6:230204/149‑6 (MQ=255) gTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGt < 4:285338/149‑1 (MQ=255) aTTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGc > 7:26210/1‑144 (MQ=255) aTTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGc < 8:26210/144‑1 (MQ=255) cttgcCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTc < 5:234299/148‑1 (MQ=255) ttgcCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTca > 3:9119/1‑148 (MQ=255) gcCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGCGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAg > 8:238557/1‑149 (MQ=255) cTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGgc > 4:255507/1‑149 (MQ=255) cTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGgc > 6:195073/1‑149 (MQ=255) cACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCGTATAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGgcg > 1:272283/1‑149 (MQ=255) aCTCTGCTCAGCTATTTCGCTCGTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACCCGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCCG‑TGTGCGTATTTTCACAGGCGcc > 7:244948/1‑149 (MQ=255) cTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGCTGTGCGTATTTTCACAGGCGCCTTgg < 1:192348/148‑1 (MQ=255) cTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTgg < 7:238557/147‑1 (MQ=255) tCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTgg > 5:146725/1‑146 (MQ=255) tCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTgg > 6:274265/1‑146 (MQ=255) aGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCtt > 5:280801/1‑142 (MQ=255) aGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGa > 3:148114/1‑148 (MQ=255) cTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACAt > 6:153651/1‑149 (MQ=255) cTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGGGCATGCCCCCGGGGGTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCGGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACAt < 6:267925/149‑1 (MQ=255) ttCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTACATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCa > 1:275598/1‑148 (MQ=255) cGCTCTTCTTTATTAAGCCTGTAGCTCTTC‑TCTAAACACGGCACCAGTTCATGCCCCCGGGGTTGCTTGGTGGCGGCTGGGTGCGGCCTTCCAGACAGGCTTGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTTGCAGACATCCaaa < 5:251327/148‑1 (MQ=255) cGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAac > 2:310539/1‑149 (MQ=255) ttctGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGt > 1:36173/1‑149 (MQ=255) ttctGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGt > 4:146609/1‑149 (MQ=255) ttctGTATTAAGCCTGTAGCGCGTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGt > 7:231717/1‑149 (MQ=255) tctGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGtt > 3:178419/1‑149 (MQ=255) tctGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGtt > 1:151614/1‑149 (MQ=255) tGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGTTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGtttt < 7:169240/149‑1 (MQ=255) tGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGtttt > 5:140374/1‑149 (MQ=255) gTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGGTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTg > 6:165035/1‑149 (MQ=255) tATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCTGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTg < 7:96426/148‑1 (MQ=255) tATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTg < 4:299981/148‑1 (MQ=255) tATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTg > 4:339051/1‑148 (MQ=255) tATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTg < 2:36173/148‑1 (MQ=255) tATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTg < 4:9119/148‑1 (MQ=255) ttAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAac < 8:288843/137‑1 (MQ=255) ttAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAac > 7:288843/1‑137 (MQ=255) ttAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTa < 1:194215/148‑1 (MQ=255) ttAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAg > 6:32387/1‑149 (MQ=255) cTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCcgcg > 3:333286/1‑149 (MQ=255) gcTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACgg > 6:125627/1‑148 (MQ=255) ttC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGCCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGa > 1:260813/1‑148 (MQ=255) c‑tctAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGAta < 8:272001/148‑1 (MQ=255) c‑tctAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGAta < 5:165035/148‑1 (MQ=255) c‑tctAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGAta < 3:205520/148‑1 (MQ=255) tAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTa < 6:337929/128‑1 (MQ=255) tAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTa > 5:337929/1‑128 (MQ=255) tAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGAtacta > 2:9704/1‑148 (MQ=255) tAAACACGACACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGAtacta > 2:269388/1‑148 (MQ=255) aaCACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGAtactac < 5:195073/147‑1 (MQ=255) aaCACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGAtactac < 5:274265/147‑1 (MQ=255) aaCACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACAt < 4:333286/149‑1 (MQ=255) cACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAg > 6:340521/1‑149 (MQ=255) aCCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAg > 1:243359/1‑148 (MQ=255) ccAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTa < 3:339051/149‑1 (MQ=255) cAGTTCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAg < 8:130424/149‑1 (MQ=255) cAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAg > 2:200078/1‑149 (MQ=255) cATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCgg < 2:260813/148‑1 (MQ=255) cATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCgg < 5:32387/148‑1 (MQ=255) aTGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCgggg < 6:146725/149‑1 (MQ=255) tGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCgggg > 4:342001/1‑148 (MQ=255) cccccGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCa > 3:300644/1‑148 (MQ=255) ccccGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCaa > 7:31228/1‑148 (MQ=255) caccGGGGTTGCTTGGGGGCGGATGGGTGCGGCCTGCAAGACAGGCTGGCGGGAAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAAAGACGTTTTGTAGTCCTCGCCAAGGGATACTACATGAAGAAGTAGGCGGGGCaa > 7:36775/3‑148 (MQ=255) cGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATc < 1:310539/149‑1 (MQ=255) gggTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCTCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAg < 3:255507/149‑1 (MQ=255) ggTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGa > 5:203080/1‑149 (MQ=255) ggTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGa < 2:243359/149‑1 (MQ=255) ttGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTaaaa < 8:31228/149‑1 (MQ=255) ttGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGAGGGCAG‑TGTGCGTATTTTCACAGGCTCCTTGGATGACATCCAAACGACGTTTTGTATTCCGCGCCACGTTATACTACATGAAGAAGTAGTCGGGGCAAAATCAGACTaaaa < 8:36775/149‑1 (MQ=255) gggggCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTaaaa < 2:272283/147‑1 (MQ=255) gggggCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTaaaa < 5:340521/147‑1 (MQ=255) ggggCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTGGGCAGACATCCAAACGCCGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGAGGGGCAAAATCAGACTAAAcgcg > 2:305317/1‑149 (MQ=255) ggcTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGtt < 5:125627/149‑1 (MQ=255) ggcTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGtt < 3:342001/149‑1 (MQ=255) gTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGa < 1:9704/149‑1 (MQ=255) gTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGa > 4:357463/1‑149 (MQ=255) acactgCCCGCCAGCCTGTCTGGCAGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTa < 7:270417/139‑1 (MQ=255) acactgCCCGCCAGCCTGTCTGGCAGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTa > 8:270417/6‑144 (MQ=255) actgCCCGCCAGCCTGTCTGGCAGGCAGTTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGAc > 1:214818/4‑149 (MQ=255) gCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGt > 2:335222/1‑149 (MQ=255) ccAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTc > 6:220240/1‑149 (MQ=255) aGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTcgc < 2:275598/149‑1 (MQ=255) aGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTcgc > 8:244088/1‑149 (MQ=255) aGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGCGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTcgc > 3:34187/1‑149 (MQ=255) gACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTcgcg > 1:207516/1‑149 (MQ=255) aCAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGt < 4:148114/149‑1 (MQ=255) aCAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGt < 8:231717/149‑1 (MQ=255) | CCACCGACTTGAACGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTC‑TCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAG‑TGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGT > NC_002947/435174‑435469 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |