| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,909,296 | A→T | 6.4% | intergenic (‑76/+93) | spuD ← / ← spuC‑II | spermidine/putrescine ABC transporter substrate‑binding protein/polyamine:pyruvate transaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,909,296 | 0 | A | T | 6.4% | 251.4 / 3.4 | 87 | intergenic (‑76/+93) | spuD/spuC‑II | spermidine/putrescine ABC transporter substrate‑binding protein/polyamine:pyruvate transaminase |
| Reads supporting (aligned to +/- strand): ref base A (39/42); new base T (4/2); total (43/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTCCACCAGT > NC_002947/5909149‑5909443 | ttCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGaaa > 6:300130/1‑149 (MQ=255)ttCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGaa > 7:235152/1‑148 (MQ=255) cTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAAACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTc > 1:261048/1‑149 (MQ=255) tGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACa > 2:224452/1‑144 (MQ=255) tGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACa < 1:224452/144‑1 (MQ=255) gAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGc > 6:160829/1‑148 (MQ=255) aaCAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGc > 2:263456/1‑147 (MQ=255) aaCAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGc < 7:335315/147‑1 (MQ=255) ggAGGCCATGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GC‑‑CTGAAACAGTCGCTAGTCTgg > 1:111396/1‑149 (MQ=255) ggCCATGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCaa < 6:194585/149‑1 (MQ=255) ggCCATGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCaa < 1:263456/149‑1 (MQ=255) cATGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGt > 5:34874/1‑148 (MQ=255) aTGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTa < 5:300130/149‑1 (MQ=255) ggCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTAc > 7:271050/1‑148 (MQ=255) gCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACaa < 5:52638/149‑1 (MQ=255) cACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAg < 8:235152/149‑1 (MQ=255) aCCCATCAGGGCGGCGGCCAGG‑AACGTGTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAgg > 1:224129/1‑149 (MQ=255) ccATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGcc > 8:118792/1‑149 (MQ=255) ccATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGcc > 3:214690/1‑149 (MQ=255) cATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGccc > 3:16335/1‑149 (MQ=255) cATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGccc < 7:316170/149‑1 (MQ=255) tCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTATTTCATGCGTTATGATCGAGAATTTTTATTAAGCAACTGTCGAGCAGCCAGATCCCAAGGTGCAGAGCGCGCGGC‑GA‑‑CTGAATCAGTCTCTAGACGGGCAAGTTACAAggacgtg > 8:331068/1‑144 (MQ=255) ggcggcggcCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCt > 8:332118/1‑144 (MQ=255) ggcggcggcCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCt < 7:332118/144‑1 (MQ=255) cggcggcCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCaa < 2:224129/149‑1 (MQ=255) ggcCAGC‑AACGTCTTGCCCATTTTATTCCTGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCAAGCAGCCAGAACCCACGGTGCAGAGAGCGCGGC‑GA‑‑CGGTTAAAGTCGCTAGTCTGGCCAGTTACACGGCCGTGGATCAAGGa > 6:345672/1‑148 (MQ=255) ggcCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGC‑CCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGaaa < 4:163517/149‑1 (MQ=255) gcCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGaaa < 3:85968/149‑1 (MQ=255) cAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGATACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTa < 8:151189/138‑1 (MQ=255) cAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAg < 8:328606/123‑1 (MQ=255) cAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAg > 7:328606/1‑123 (MQ=255) cAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTa > 7:151189/1‑138 (MQ=255) cAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGc < 5:160829/149‑1 (MQ=255) aaCGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAgg < 8:84399/148‑1 (MQ=255) aaCGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAgg < 3:232890/148‑1 (MQ=255) aCGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGc > 1:66844/1‑149 (MQ=255) cGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTg < 6:234875/112‑1 (MQ=255) cGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGcc < 8:271050/149‑1 (MQ=255) tCTTGACCATTTTATTCATGCGTAATGCTACAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCAt > 6:333435/1‑149 (MQ=255) cccATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATATAAc < 2:111396/149‑1 (MQ=255) cATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGa < 7:118792/149‑1 (MQ=255) cATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGa < 2:180755/149‑1 (MQ=255) tGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGCGGAGGCTGAAACAGTCGCTAGTCTGGCAAGTTAAAAGGCCCTGTATCAAGAAAAGCAGGGCCATGTAACGACTTAATGt > 1:299969/1‑149 (MQ=255) aaTGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGaaa > 6:8783/1‑116 (MQ=255) aaTGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGaaa < 5:8783/116‑1 (MQ=255) tGCTCCAGAATTTTTATTTAGCAACTCTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACACTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGcta < 5:90834/149‑1 (MQ=255) gCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGctag > 5:309360/1‑149 (MQ=255) tCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGctagcc > 2:288058/1‑149 (MQ=255) tCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGctagcc > 8:92222/1‑149 (MQ=255) ccAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGctagcc > 3:364086/1‑148 (MQ=255) cAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGTC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGctagc < 5:333435/146‑1 (MQ=255) gAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGctagcctagc < 1:306147/149‑1 (MQ=255) tttttATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCaaa > 1:121558/1‑149 (MQ=255) tttatttaGCAACTTTCCAGCAGCCAGAACCCACTGTTCAGAGCGCGCGGC‑GA‑‑CTGAAACATTAGGTAGTCTGGCAATTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAAc < 5:345672/148‑1 (MQ=255) tatttaGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCt > 7:231301/1‑149 (MQ=255) taGCAACTGTCCAGCAGACAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAACGTCACCACGCTAGCCTAGCAAACGCTTAtt > 2:188045/1‑149 (MQ=255) aCTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATc > 4:59623/1‑149 (MQ=255) agcagcCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCaa < 7:92222/149‑1 (MQ=255) gcagcCAGAACACACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAg < 3:59623/149‑1 (MQ=255) cagcCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAgg > 2:230686/1‑149 (MQ=255) agcCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGCCCCTGTATCAAGGAAAGCAGGGCCTTGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAggg > 5:296696/1‑149 (MQ=255) ccAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTc < 8:231301/149‑1 (MQ=255) aGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑Ag < 6:309360/149‑1 (MQ=255) ccACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTc > 5:168421/1‑147 (MQ=255) cACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAg < 4:364086/149‑1 (MQ=255) cAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAACGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTg < 4:214690/149‑1 (MQ=255) agagCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTgc > 7:1946/1‑149 (MQ=255) agagCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGACTCAAGGGTC‑AGGTCCAGGCACTTgc > 2:184844/1‑149 (MQ=255) agCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTgcgc > 7:82856/1‑149 (MQ=255) agactagc‑gA‑‑CTGTTTCAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTgcgcgc > 1:355043/7‑149 (MQ=255) gactagc‑gA‑‑CTGTTTCAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGc > 5:2483/6‑138 (MQ=255) gactagc‑gA‑‑CTGTTTCAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGc < 6:2483/133‑1 (MQ=255) gcggc‑gA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCtt > 5:43570/1‑149 (MQ=255) tagc‑gA‑‑CTGTTTCAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCttt > 7:349602/3‑149 (MQ=255) ggc‑gA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATc > 7:267040/1‑112 (MQ=255) ggc‑gA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATc < 8:267040/112‑1 (MQ=255) c‑gA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCATGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTc < 5:375267/148‑1 (MQ=255) c‑gA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCATGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTc > 6:375267/1‑148 (MQ=255) c‑gA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTcc < 2:299969/149‑1 (MQ=255) a‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTccac < 1:288058/149‑1 (MQ=255) a‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTccac < 4:16335/149‑1 (MQ=255) a‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTccac < 6:43570/149‑1 (MQ=255) a‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTccac < 2:121558/149‑1 (MQ=255) tGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTc > 3:316410/1‑118 (MQ=255) tGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTc < 4:316410/118‑1 (MQ=255) tGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTccac > 1:160192/1‑147 (MQ=255) tGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTccac < 2:160192/147‑1 (MQ=255) tGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCAGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTc > 8:297442/1‑144 (MQ=255) tGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCAGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTc < 7:297442/144‑1 (MQ=255) aaaCAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTCCACCAGt > 3:46751/1‑149 (MQ=255) aaaCAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTCCACCAGt > 7:207168/1‑149 (MQ=255) aaCAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTCTAGGTCCAGGCACTTGCGCGCCTTTTCCACCAGt < 1:230686/149‑1 (MQ=255) | TTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGC‑AACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTATTTAGCAACTGTCCAGCAGCCAGAACCCACGGTGCAGAGCGCGCGGC‑GA‑‑CTGAAACAGTCGCTAGTCTGGCAAGTTACAAGGCCCTGTATCAAGGAAAGCAGGGCCATGTAACGACTTAATGTCACAACGCTAGCCTAGCAAACGCTTATTGAATCGCCTCAAGGGTC‑AGGTCCAGGCACTTGCGCGCCTTTTCCACCAGT > NC_002947/5909149‑5909443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |