Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,917,153	A→G	6.1%	K71E (AAG→GAG)	ygiF →	thiamine triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,917,153	0	A	G	6.1%	247.1 / 3.9	83	K71E (AAG→GAG)	ygiF	thiamine triphosphatase
Reads supporting (aligned to +/- strand):  ref base A (36/41);  new base G (2/3);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCTGCTGAAAAAGCGCAACAAGTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTGGCCAACCTGGACAAGAAAACCATCAAGCCG  >  NC_002947/5917009‑5917299                                                                                                                                                 |                                                                                                                                                   ccTCTGCTGAAAAAGCGCAACAAGTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTg                                                                                                                                                >  8:283899/1‑149 (MQ=255)     tgctgAAAAAGCGCAACAAGTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCcgc                                                                                                                                            <  3:277326/149‑1 (MQ=255)        tgAAAAAGCGCAACAAGTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGc                                                                                                                                         >  2:53975/1‑149 (MQ=255)         gAAAAAGCGCAACAAGTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGc                                                                                                                                         >  4:172194/1‑148 (MQ=255)            aaaGCGCAACAAGTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCaga                                                                                                                                     <  5:184801/149‑1 (MQ=255)                 gcAACAAGTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGCCACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTg                                                                                                                                >  3:2891/1‑149 (MQ=255)                 gcAACAAGTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCTCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTg                                                                                                                                <  7:324393/149‑1 (MQ=255)                   aacaaGTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTggc                                                                                                                              >  3:261420/1‑149 (MQ=255)                    acaaGTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTggcc                                                                                                                             <  2:346997/149‑1 (MQ=255)                      aaGTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCG‑CCTGCGCCATCGCCGCCAAGGCGACGCGGGGCTTAAAACCCACCACTGCCCCCGCCACCGCCTggccggc                                                                                                                          >  7:119954/1‑149 (MQ=255)                       aGTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTggccggc                                                                                                                          <  7:229423/149‑1 (MQ=255)                        gTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTggccggcc                                                                                                                         >  7:150142/1‑149 (MQ=255)                         tCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCt                                                                                                                        <  1:53975/149‑1 (MQ=255)                           cGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGt                                                                                                                      >  5:3943/1‑149 (MQ=255)                             gCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTcc                                                                                                                    <  5:177243/149‑1 (MQ=255)                                 gCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTggcc                                                                                                                             <  5:304893/136‑1 (MQ=255)                                 gCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTggcc                                                                                                                             >  6:304893/1‑136 (MQ=255)                                  cAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACg                                                                                                               >  3:267363/1‑149 (MQ=255)                                   aGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGc                                                                                                              <  6:325342/149‑1 (MQ=255)                                      cccGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAAc                                                                                                           <  2:267486/149‑1 (MQ=255)                                      cccGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAAc                                                                                                           <  7:283899/149‑1 (MQ=255)                                      cccGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAAc                                                                                                           <  8:150142/149‑1 (MQ=255)                                         gTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAg                                                                                                        <  3:293313/149‑1 (MQ=255)                                          tGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGt                                                                                                       <  4:261420/149‑1 (MQ=255)                                              cTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCGAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACg                                                                                                    >  2:110041/1‑148 (MQ=255)                                               tGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGaa                                                                                                  >  8:34079/1‑149 (MQ=255)                                               tGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGaa                                                                                                  >  5:151046/1‑149 (MQ=255)                                                gCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAAt                                                                                                 >  4:289710/1‑149 (MQ=255)                                                 cTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAAt                                                                                                 <  5:247626/148‑1 (MQ=255)                                                  tCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCGAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATg                                                                                                >  8:128565/1‑148 (MQ=255)                                                  tCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGAGCCTGCGCCGCGATGGCGACGCTGTCATTAAAACCCTCAAGTGCCGCGGACAGAGCGTGGCCGGCCTGTCCGAACGAAACGAGTACGAATg                                                                                                >  7:289267/1‑148 (MQ=255)                                                  tCAACCAGTACTTCGACACCCCCGAGCGAGAGCTCTCAGCCGCCCGTCACGCCCTGCGCCAGCGCCGAGATGGCGACGCTCTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGAGGCCGGCCTGTCCGAACGCAACGAGTACGAATg                                                                                                >  3:145577/1‑148 (MQ=255)                                                    aaCCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGa                                                                                              <  2:118104/148‑1 (MQ=255)                                                     aCCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAAc                                                                                            <  5:78650/149‑1 (MQ=255)                                                               cGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAg                                                                                   <  3:3493/148‑1 (MQ=255)                                                                gACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGt                                                                                 <  4:356237/149‑1 (MQ=255)                                                                   agccccGAGCGTGAGCTGTCCGGCGCCCGTGTCGCTCTGCGCCTGCGGCGCGATGGCGACGCTGTCATTGAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTTCGAATGGAAGCTGGACAAGGTCaa                                                                              <  4:145577/147‑1 (MQ=255)                                                                    cccccGAGCGTGAGCTGTCCGCCGCCCGTGTCTCCGTGCGCCTGCGCCGGGATTGCTACGCGGTCATGAAAACGCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGGACGAAACGAGTACGAATGGAACCTGGACAAGGTCAAg                                                                             <  4:274060/149‑1 (MQ=255)                                                                      cccGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCt                                                                           >  3:168436/1‑149 (MQ=255)                                                                      cccGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCt                                                                           >  2:35363/1‑149 (MQ=255)                                                                      cccGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCt                                                                           >  5:153340/1‑149 (MQ=255)                                                                       ccGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGt                                                                                                       >  7:33282/1‑120 (MQ=255)                                                                       ccGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGt                                                                                                       <  8:33282/120‑1 (MQ=255)                                                                       ccGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTc                                                                          >  3:100977/1‑149 (MQ=255)                                                                             gTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGt                                                                                                                      >  4:245223/1‑99 (MQ=255)                                                                             gTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGt                                                                                                                      <  3:245223/99‑1 (MQ=255)                                                                              tGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTga                                                                   <  4:267363/149‑1 (MQ=255)                                                                                aGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGaa                                                                                                  <  1:227080/116‑1 (MQ=255)                                                                                aGCTGTCCGCCGCCCGTGTCGCCCTGCGCCAGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGaa                                                                                                  >  2:227080/1‑116 (MQ=255)                                                                                  cTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTgaagaa                                                               <  4:100977/149‑1 (MQ=255)                                                                                     gccgcggCCCGGGT‑GGCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCGAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTg                                                           <  8:119954/148‑1 (MQ=255)                                                                                       cgccgcCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGGACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTg                                                           >  2:356565/1‑148 (MQ=255)                                                                                       cgccgcCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTgg                                                          <  4:168436/149‑1 (MQ=255)                                                                                             ccGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCa                                                    <  3:172194/149‑1 (MQ=255)                                                                                                  tCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGaagaag                                                              <  3:45105/134‑1 (MQ=255)                                                                                                  tCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGaagaag                                                              >  4:45105/1‑134 (MQ=255)                                                                                                    gCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGCGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACctgctg                                             >  5:116209/1‑149 (MQ=255)                                                                                                    gCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGATTGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACctgctg                                             >  1:302929/1‑149 (MQ=255)                                                                                                    gCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTgg                                                                                        <  8:271725/106‑1 (MQ=255)                                                                                                    gCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTgg                                                                                        >  7:271725/1‑106 (MQ=255)                                                                                                      cctgcgcctgcgCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTg                                                                    >  5:63948/1‑124 (MQ=255)                                                                                                      cctgcgcctgcgCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTg                                                                    <  6:63948/124‑1 (MQ=255)                                                                                                      cctgcgcctgcgCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGc                                           <  6:153340/149‑1 (MQ=255)                                                                                                       ctgcgcctgcgcCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGcc                                          >  4:327331/1‑149 (MQ=255)                                                                                                          cgcctgcgccGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGa                                       <  3:289710/149‑1 (MQ=255)                                                                                                          cgcctgcgccGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGa                                       <  7:34079/149‑1 (MQ=255)                                                                                                           gcctgcgccGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAg                                      >  6:77680/1‑149 (MQ=255)                                                                                                           gcctgcgccGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAg                                      <  6:3943/149‑1 (MQ=255)                                                                                                           gcctgcgccGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAg                                      >  6:11279/1‑149 (MQ=255)                                                                                                                cgccgcGATGGCGACGCTGTCATTCAAACCCTCGAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCaa                                   <  1:110041/147‑1 (MQ=255)                                                                                                                  ccgcGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTgg                               >  1:165425/1‑149 (MQ=255)                                                                                                                   cgcgATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGCATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTGGc                              <  4:2891/149‑1 (MQ=255)                                                                                                                   cgcgATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTGGc                              <  5:77680/149‑1 (MQ=255)                                                                                                                          gCGACGCTGTCATTCAAACCCTCGAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTGGCCAACCt                        <  7:128565/148‑1 (MQ=255)                                                                                                                          gCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTGGCCAACCTg                       >  3:253270/1‑149 (MQ=255)                                                                                                                          gCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTGGCCAACCTg                       <  1:35363/149‑1 (MQ=255)                                                                                                                            gACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTGGCCAACCTGGa                     >  2:64575/1‑149 (MQ=255)                                                                                                                               gCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTGGCCAACCTGGAc                    >  8:277804/1‑147 (MQ=255)                                                                                                                                  gTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTGGCCAACCTGGACAAGa                >  3:65289/1‑148 (MQ=255)                                                                                                                                         aaaCCCTCAAGTGCCTCGGACAGAGCGTGGCCGGGATGTCCGAACGCAACGAGTACGAATGGAACATGTACAATGTCAAGCTCGACCTGAAGAAGATTGACTCCACCTTCTGGCCCGATCAACTGGCCAACCTGGACAAGAAAACCATc        <  8:289267/149‑1 (MQ=255)                                                                                                                                         aaaCCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTGGCCAACCTGGACAAGAAAACCATc        <  7:277804/149‑1 (MQ=255)                                                                                                                                             ccTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTGGCCAACCTGGACAAGAAAACCATCAAGc    <  1:164465/149‑1 (MQ=255)                                                                                                                                               tCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTGGCCAACCTGGACAAGAAAACCATCAAGCCg  >  7:47256/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CCTCTGCTGAAAAAGCGCAACAAGTCCGGCTGGCAGACCCGTGAACTGCTCAACCAGTACTTCGACACCCCCGAGCGTGAGCTGTCCGCCGCCCGTGTCGCCCTGCGCCTGCGCCGCGATGGCGACGCTGTCATTCAAACCCTCAAGTGCCGCGGCCAGAGCGTGGCCGGCCTGTCCGAACGCAACGAGTACGAATGGAACCTGGACAAGGTCAAGCTCGACCTGAAGAAGCTGGACGCCACCTGCTGGCCCGAGCAACTGGCCAACCTGGACAAGAAAACCATCAAGCCG  >  NC_002947/5917009‑5917299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑