Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,934,056	G→T	6.1%	intergenic (‑80/‑66)	PP_5201 ← / → PP_5721	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,934,056	0	G	T	6.1%	211.6 / 2.9	65	intergenic (‑80/‑66)	PP_5201/PP_5721	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/29);  new base T (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TGACAGGATGGCCATTGCTCGAGAGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGATCGAACGCGTCGAGCAACTAAAACG  >  NC_002947/5933910‑5934204                                                                                                                                                     |                                                                                                                                                     tGACAGGATGGCCATTGCTCGAGAGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCgg                                                                                                                                                     >  1:72938/1‑148 (MQ=255)     aGGATGGCCATTGCTCGAGAGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACGTTGTATCCTAGCACCGGCAGGCGCGCCC‑‑AGACCATCGGCACTg                                                                                                                                                >  6:365583/1‑149 (MQ=255)           gCCATTGCTCGAGAGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGt                                                                                                                                           <  2:39515/148‑1 (MQ=255)            ccATTGCTCGAGAGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTg                                                                                                                                         <  2:340118/149‑1 (MQ=255)              aTTGCTCGAGAGCAGCATGGCGAAAGCGATGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTgcc                                                                                                                                       >  1:207756/1‑149 (MQ=255)                 gCTCGAGAGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTgccgcc                                                                                                                                    >  6:137677/1‑149 (MQ=255)                   tCGAGAGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCTGCACTGGCCGTTgccgcc                                                                                                                                    >  4:266835/1‑147 (MQ=255)                   tCGAGAGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCTGCACTGGCCGTTgccgcc                                                                                                                                    >  4:271837/1‑147 (MQ=255)                   tCGAGAGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGGCTAGAATGAAGACCTTGTATCATAGCACCGGCAGGCGCGCCA‑‑AGACAATCGGCACTGGCCGTTgccgcc                                                                                                                                    >  7:97141/1‑147 (MQ=255)                     gagaGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAgg                                                                                                                                <  2:173828/149‑1 (MQ=255)                        agcagcATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGACTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGCga                                                                                                                              >  3:89219/1‑148 (MQ=255)                          cagcaTGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGgaga                                                                                                                            <  8:129108/148‑1 (MQ=255)                            gcaTGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAgg                                                                                                                          >  7:325672/1‑148 (MQ=255)                            gcaTGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGc                                                                                                                         <  2:207756/149‑1 (MQ=255)                             caTGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGca                                                                                                                        <  7:12744/149‑1 (MQ=255)                               tGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGcatc                                                                                                                      <  8:325672/149‑1 (MQ=255)                                  cGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCatcatc                                                                                                                   <  5:59271/149‑1 (MQ=255)                                   gAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCg                                                                                                                  >  6:325849/1‑149 (MQ=255)                                         gTTGTAGGGCTATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGt                                                                                                            <  5:46511/149‑1 (MQ=255)                                          ttGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTg                                                                                                           <  2:72938/149‑1 (MQ=255)                                               gggCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTc                                                                                                       >  1:325194/1‑148 (MQ=255)                                                          tATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGc                                                                                            >  6:60697/1‑148 (MQ=255)                                                            ttGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGaa                                                                                         >  8:304638/1‑149 (MQ=255)                                                                gcatgggcaGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGa                                                                                     <  1:165519/149‑1 (MQ=255)                                                                    gggcaGCTAGGCGCCAGACGGCGCAATGTCGAGAATGAAGACCTTGTATCCTAGCACCGCCAGGCGCGCCA‑‑AGACCCTCGGCACTGGCCGTTGCCGCAGAGGCAGAGGCATCATCGCCAGGTGAGTCAACGCCGTAGCGCATCCAGAGc                                                                                 >  8:315438/1‑149 (MQ=255)                                                                     ggcaGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGc                                                                                 <  2:325194/148‑1 (MQ=255)                                                                      gcaGCTAGGCGCCAGACGGCGCAATGTCTTGAATGAAGACCTTGTATCCTCGCACCGGCAGGCGCGCCA‑‑AGACCCTCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGGGAGGCAACGACGGAGCGAATCGAGTGCaa                                                                               <  4:56723/149‑1 (MQ=255)                                                                      gcaGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCTGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCaa                                                                               <  3:271837/149‑1 (MQ=255)                                                                      gcaGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCTGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCaa                                                                               <  3:266835/149‑1 (MQ=255)                                                                         gCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAAccc                                                                            <  7:3882/149‑1 (MQ=255)                                                                            aGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACg                                                                         >  7:3171/1‑149 (MQ=255)                                                                                gcCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGa                                                                                              <  7:215140/124‑1 (MQ=255)                                                                                gcCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGa                                                                                              >  8:215140/1‑124 (MQ=255)                                                                                gcCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGaa                                                                                         >  7:16652/1‑129 (MQ=255)                                                                                gcCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGaa                                                                                         <  8:16652/129‑1 (MQ=255)                                                                                 ccAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCAAGAGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCACGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCaa                                                                      >  1:290244/1‑149 (MQ=255)                                                                                     aCGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAAc                                                                <  5:137677/149‑1 (MQ=255)                                                                                       ggCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGcatcat                                                                                                                    >  4:213223/1‑95 (MQ=255)                                                                                       ggCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGcatcat                                                                                                                    <  3:213223/95‑1 (MQ=255)                                                                                        gcgcAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGAc                                                             <  5:332906/149‑1 (MQ=255)                                                                                          gcAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGACCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCAACGCAAGAGAACGACCt                                                           >  5:110921/1‑149 (MQ=255)                                                                                               gTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAgg                                                      >  7:42210/1‑149 (MQ=255)                                                                                                        gAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAgg                                                      <  6:12707/140‑1 (MQ=255)                                                                                                        gAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAgg                                                      >  5:12707/1‑140 (MQ=255)                                                                                                        gAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACtgat                                               >  5:298347/1‑147 (MQ=255)                                                                                                         aaGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCa                                                    >  4:170839/1‑141 (MQ=255)                                                                                                         aaGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCa                                                    <  3:170839/141‑1 (MQ=255)                                                                                                         aaGACCTTGAATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACtgatga                                             <  5:365583/148‑1 (MQ=255)                                                                                                           gACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCTCGCAAGAGAACGACCTGCAGGCACTGATGAGcc                                          >  3:188607/1‑149 (MQ=255)                                                                                                              cTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGAt                                       >  1:223798/1‑149 (MQ=255)                                                                                                                       tAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGAACTGGCCGTTGCCGCCCAGGGAGAGGCATCATAGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCCCGCAAGAGAACGACCAGCAGGCACTGATGAGACGATTCGAGTTg                               >  5:262038/1‑148 (MQ=255)                                                                                                                         gCACCGGCAGTCGCGCAA‑‑AGCCCATCGGCTATGGCCGTTGCCGACCAGGGAGAGGCTTCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACACACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGc                              <  6:262038/147‑1 (MQ=255)                                                                                                                           aCCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGAt                          >  2:220821/1‑149 (MQ=255)                                                                                                                           aCCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGAt                          >  2:6992/1‑149 (MQ=255)                                                                                                                            ccGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGATc                         <  5:325849/149‑1 (MQ=255)                                                                                                                             cGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGt                                                                                   >  7:110190/1‑90 (MQ=255)                                                                                                                             cGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGt                                                                                   <  8:110190/90‑1 (MQ=255)                                                                                                                                     gcgcCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGATCGAACGCGTc                <  5:60697/149‑1 (MQ=255)                                                                                                                                      cgcCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGATCGAACGCGTCg               <  2:290244/149‑1 (MQ=255)                                                                                                                                       gcCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGATCGAACGCGTCGa              <  6:298347/149‑1 (MQ=255)                                                                                                                                         cA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGCCCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGATCGAACGGGTCGAg             >  7:226206/1‑148 (MQ=255)                                                                                                                                         cA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGCTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGATCGAACGCGTCGAg             >  5:331048/1‑148 (MQ=255)                                                                                                                                          a‑‑aGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGCGCGAATCGAGTGCACCCCCCGCAAGCGACCGACCTGCCGGCCCTGATGAGCCGATTCGAGTTGCTGATCGAACGCGTCGAGCa           >  3:207326/1‑149 (MQ=255)                                                                                                                                             aGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGATCGAACGCGTCGAGCaa          <  8:42210/149‑1 (MQ=255)                                                                                                                                              gACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGATCGAACGCGTCGAGCAAc         >  4:339143/1‑149 (MQ=255)                                                                                                                                                    cGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGATCGAACGCGTCGAGCAACTAAAAc   >  4:128182/1‑149 (MQ=255)                                                                                                                                                     ggCTCTGGCAGTTGCCGCCCATGGAGAGGCATCATCGCCAGGTGAGTCGACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGATCGACCTGCGGGCACTGATGAGCCGATTCGAGTTGCTGATCGAACGCGTCGAGCAACTAAAACg  <  4:89219/149‑1 (MQ=255)                                                                                                                                                     ggCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCTAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGATCGAACGCGTCGAGCAACTAAAACg  <  6:331048/149‑1 (MQ=255)                                                                                                                                                     ggCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGATCGAACGCGTCGAGCAACTAAAACg  <  3:339143/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TGACAGGATGGCCATTGCTCGAGAGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCAGACGGCGCAATGTCTAGAATGAAGACCTTGTATCCTAGCACCGGCAGGCGCGCCA‑‑AGACCATCGGCACTGGCCGTTGCCGCCCAGGGAGAGGCATCATCGCCAGGTGAGTCAACGACGGAGCGAATCGAGTGCAACCCACGCAAGAGAACGACCTGCAGGCACTGATGAGCCGATTCGAGTTGCTGATCGAACGCGTCGAGCAACTAAAACG  >  NC_002947/5933910‑5934204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑