Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 138,950 T→G 6.8% R518S (AGA→AGC)  kinB ← alginate biosynthesis sensor protein KinB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947138,9500TG 6.8% 242.2 / 3.8 82R518S (AGA→AGC) kinBalginate biosynthesis sensor protein KinB
Reads supporting (aligned to +/- strand):  ref base T (35/41);  new base G (3/3);  total (38/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATTTCCTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACTCGATCTGC  >  NC_002947/138803‑139094
                                                                                                                                                   |                                                                                                                                                
aaTTTCCTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTc                                                                                                                                                 >  8:168580/1‑149 (MQ=255)
aaTTTCCTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTc                                                                                                                                                 >  3:174035/1‑149 (MQ=255)
aaTTTCCTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTc                                                                                                                                                 >  5:189693/1‑149 (MQ=255)
  tttCCTTTCACAGGGCGTGCCCCAGCCCAGGGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTg                                                                                                                                               <  7:323265/149‑1 (MQ=255)
  tttCCTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTg                                                                                                                                               <  3:65208/149‑1 (MQ=255)
   ttCCTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTgg                                                                                                                                              >  3:81617/1‑149 (MQ=255)
    tCCTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTgg                                                                                                                                              <  5:92196/148‑1 (MQ=255)
      cTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCGTTTCTTGCGACCTACCTGCACGTAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCAt                                                                                                                                           <  5:57269/149‑1 (MQ=255)
      cTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCAt                                                                                                                                           <  2:45947/149‑1 (MQ=255)
       ttGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATg                                                                                                                                          >  1:178122/1‑149 (MQ=255)
            caGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGc                                                                                                                                     <  1:369923/149‑1 (MQ=255)
             aGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCg                                                                                                                                    >  2:363666/1‑149 (MQ=255)
                  gAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATgc                                                                                                                               >  2:130146/1‑149 (MQ=255)
                   aGCCCCCGCCCAGCGACGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATgcg                                                                                                                              <  3:19739/149‑1 (MQ=255)
                      cccagcccagCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTCCTGCATGCCAGACGGCATGCAgaacgggt                                                                                                                                <  5:19422/144‑8 (MQ=255)
                      cccagcccagCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTCCTGCATGCCAGACGGCATGCAgaacgggt                                                                                                                                >  6:19422/1‑137 (MQ=255)
                       ccagcccagcGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGcgcccg                                                                                                                          <  1:130146/149‑1 (MQ=255)
                        cagcccagcGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGcgcccgc                                                                                                                         >  6:29316/1‑149 (MQ=255)
                            ccagcGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCGGGCGCATCcgcctgcatgc                                                                                                                     <  1:146989/149‑5 (MQ=255)
                              agcGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGGCTGGCATGCAGGCGGATGCGCCCGCCGTTgg                                                                                                                   <  8:71662/149‑1 (MQ=255)
                                cgccgcCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCg                                                                                                                 <  5:305171/149‑1 (MQ=255)
                                cgccgcCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCg                                                                                                                 <  6:340432/149‑1 (MQ=255)
                                cgccgcCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCg                                                                                                                 <  3:215489/149‑1 (MQ=255)
                                 gccgccTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCgg                                                                                                                <  4:174035/149‑1 (MQ=255)
                                  ccgccTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGt                                                                                                               <  1:363666/149‑1 (MQ=255)
                                    gccTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTAt                                                                                                             >  5:313573/1‑149 (MQ=255)
                                         tCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGc                                                                                                          <  1:32457/147‑1 (MQ=255)
                                                 cctacctGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGtt                                                                                                <  3:153821/149‑1 (MQ=255)
                                                     cctGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTgc                                                                                            <  6:189693/149‑1 (MQ=255)
                                                      ctGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTtgtg                                                                                             >  7:262430/1‑147 (MQ=255)
                                                      ctGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTtgtg                                                                                             >  4:10014/1‑147 (MQ=255)
                                                         cACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTgcagca                                                                                        <  2:352287/149‑1 (MQ=255)
                                                            gAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTgcagca                                                                                        >  6:145250/1‑146 (MQ=255)
                                                            gAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTgcagca                                                                                        <  5:145250/146‑1 (MQ=255)
                                                             aaaGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGtt                                                                                    <  7:291606/149‑1 (MQ=255)
                                                                ggCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTc                                                                                 <  2:178122/149‑1 (MQ=255)
                                                                  cTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGTAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTc                                                                                 >  1:113494/1‑147 (MQ=255)
                                                                  cTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCa                                                                               <  5:29316/149‑1 (MQ=255)
                                                                  cTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTATCCGTTGTCCTCGACGCTGATTATCACCCGTTCTGCATGCCTTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCa                                                                               <  2:109652/149‑1 (MQ=255)
                                                                   tCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCGTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGGAGCAGGTTGTCCAg                                                                              >  8:61174/1‑149 (MQ=255)
                                                                   tCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAg                                                                              >  4:15376/1‑149 (MQ=255)
                                                                     gAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGt                                                                                                               >  2:199933/1‑114 (MQ=255)
                                                                     gAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGt                                                                                                               <  1:199933/114‑1 (MQ=255)
                                                                           gcgcgcTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATggtg                                                                                                  <  4:279738/121‑3 (MQ=255)
                                                                           gcgcgcTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATggag                                                                                                  >  3:279738/1‑119 (MQ=255)
                                                                             gcgcTTGTTGCCCATAGGAAATGCCATATCCGTAGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGCTGCGCCCGCCGTTGGCGGAATGGCGGAAGGCGTTGTGCACCAGGATGTCCAGCACTCGGTcc                                                                    >  8:300655/1‑149 (MQ=255)
                                                                               gcTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCaa                                                                  <  5:4931/149‑1 (MQ=255)
                                                                                cttctcgCCCATAGCAAATGCCCGCTCCGTTCTCCTCGACGCTGCTGATCACCCGTTCTGCCTGCCGGCTGGCAGGCAGGCGGATGCGCCCGCCGTGGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAAc                                                                 <  8:262430/143‑1 (MQ=255)
                                                                                    ttgCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGTAGGTTGTCCAGCACTCGGTCCAACTggg                                                             <  1:357416/149‑1 (MQ=255)
                                                                                    ttgCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTgg                                                              <  4:81617/148‑1 (MQ=255)
                                                                                      gCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCg                                                           >  8:261382/1‑149 (MQ=255)
                                                                                       cccATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGa                                                          <  7:168580/149‑1 (MQ=255)
                                                                                        ccATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGAc                                                         >  1:357713/1‑149 (MQ=255)
                                                                                        ccATAGGAAATGCCCTCTCCGTTGTCCTCGAAGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGAc                                                         >  1:13167/1‑149 (MQ=255)
                                                                                            aGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATGACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCg                                                     <  8:111688/149‑1 (MQ=255)
                                                                                                    cccTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGtt                                                                                                >  1:283991/1‑98 (MQ=255)
                                                                                                    cccTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGtt                                                                                                <  2:283991/98‑1 (MQ=255)
                                                                                                        ctcCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATAcgc                                         >  7:145783/1‑149 (MQ=255)
                                                                                                           cGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGCCGTCGGCCTGGATACGCgg                                       >  2:361890/1‑148 (MQ=255)
                                                                                                                  cTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAgcggcg                               >  1:314850/1‑149 (MQ=255)
                                                                                                                  cTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAgcggc                                <  3:10014/148‑1 (MQ=255)
                                                                                                                  cTCGACGCTGATGATAACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAgcggcg                               >  6:107780/1‑149 (MQ=255)
                                                                                                                   tCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGcggcgg                              >  4:227881/1‑149 (MQ=255)
                                                                                                                    cGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGCTACGCGGTAGCGGCggg                             >  8:231422/1‑149 (MQ=255)
                                                                                                                          tgatgaTCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCg                                                           >  2:221736/1‑113 (MQ=255)
                                                                                                                          tgatgaTCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCg                                                           <  1:221736/113‑1 (MQ=255)
                                                                                                                            atgatCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGt                       <  2:13167/147‑1 (MQ=255)
                                                                                                                               atCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCtt                  <  6:83392/149‑1 (MQ=255)
                                                                                                                               atCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCt                   <  6:313573/148‑1 (MQ=255)
                                                                                                                                  aCCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAAc                                                                 <  8:247382/99‑1 (MQ=255)
                                                                                                                                  aCCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGAt               >  6:380381/1‑149 (MQ=255)
                                                                                                                                   cccGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGcggcgg                              >  7:212044/1‑133 (MQ=255)
                                                                                                                                   cccGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGcggcgg                              <  8:212044/133‑1 (MQ=255)
                                                                                                                                   cccGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGAt               >  5:361708/1‑148 (MQ=255)
                                                                                                                                    ccGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATc              >  1:338090/1‑148 (MQ=255)
                                                                                                                                       ttCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACt          <  3:15376/149‑1 (MQ=255)
                                                                                                                                        ccTGCATGCCAGACGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACTc         >  4:295723/2‑149 (MQ=255)
                                                                                                                                         cTGCATGCCAGACGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGAGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTCGGCGACGTCGGCAAGGATACGCGGTAGCGGCGGGTCCAATTCCTTGATaaaaac         >  4:38918/1‑145 (MQ=255)
                                                                                                                                          tGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAAc           >  8:190265/1‑145 (MQ=255)
                                                                                                                                           gCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACTc         >  6:68829/1‑146 (MQ=255)
                                                                                                                                                ccGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACTCGATCTGc  <  8:145783/148‑1 (MQ=255)
                                                                                                                                                ccGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACTCGATCTGc  <  7:190265/148‑1 (MQ=255)
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AATTTCCTTGCACAGCGCGAGCCCCAGCCCAGCGCCGCCTTTCTTGCGACCTACCTGCACGAAAGGCTCGAAGATGCGCGCTTGTTGCCCATAGGAAATGCCCTCTCCGTTGTCCTCGACGCTGATGATCACCCGTTCTGCATGCCGTCTGGCATGCAGGCGGATGCGCCCGCCGTTGGCGGTATGGCGGATGGCGTTGTGCAGCAGGTTGTCCAGCACTCGGTCCAACTGGGCGACGTCGGCCTGGATACGCGGTAGCGGCGGGTCCAGTTCCTTGATCAACTCGATCTGC  >  NC_002947/138803‑139094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑