Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,001,228 G→T 7.7% P358Q (CCG→CAG)  amaA ← L‑pipecolate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,001,2280GT 7.7% 163.4 / 3.8 52P358Q (CCG→CAG) amaAL‑pipecolate oxidase
Reads supporting (aligned to +/- strand):  ref base G (22/26);  new base T (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

GCTCACACCGCTCATCGGCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTC  >  NC_002947/6001083‑6001373
                                                                                                                                                 |                                                                                                                                                 
gcTCACACCGCTCATCGGCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCt                                                                                                                                                >  7:116908/1‑149 (MQ=255)
gcTCACACCGCTCATCGGCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCt                                                                                                                                                <  8:189315/149‑1 (MQ=255)
    acacCGCTCATCGGCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGaa                                                                                                                                            <  2:127231/149‑1 (MQ=255)
       ccGCTCATCGGCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAg                                                                                                                                         <  2:42687/149‑1 (MQ=255)
         gCTCATCGGCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAAACACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCgg                                                                                                                                                  <  8:287871/138‑1 (MQ=255)
         gCTCATCGGCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAAACACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCgg                                                                                                                                                  >  7:287871/1‑138 (MQ=255)
              tCGGCGAAAACTGAATCGGCAGGCGGTCCGCCTGGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATAACGGTGCCGGTCGTGTTACCCCGACCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCTGTTCGAGCCGgcgcctg                                                                                                                                  <  7:164202/149‑4 (MQ=255)
              tCGGCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCAt                                                                                                                                   >  2:285895/1‑148 (MQ=255)
                ggCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAg                                                                                                                                <  3:221581/149‑1 (MQ=255)
                ggCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAg                                                                                                                                >  6:1316/1‑149 (MQ=255)
                 gCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAgg                                                                                                                               <  4:263114/149‑1 (MQ=255)
                   gAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCACTCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAgggg                                                                                                                             >  7:347703/1‑147 (MQ=255)
                   gAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACCGTGCCGGTCGTCTTACCCCGCCCGTTGTACCCAGTCACCGCCACCACCCCAGGCGCCGGATCGAACAGGCGCATCAggtg                                                                                                                             >  3:30596/1‑149 (MQ=255)
                     aaaCGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAggtggt                                                                                                                           <  6:300695/149‑1 (MQ=255)
                      aaCGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAggtggt                                                                                                                           >  1:9405/1‑148 (MQ=255)
                        cGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAg                                                                                                                        <  6:352441/149‑1 (MQ=255)
                         ggAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCTGTTCGAGCCGgc                                                                                                                                       >  1:190431/1‑133 (MQ=255)
                         ggAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCTGTTCGAGCCGgc                                                                                                                                       <  2:190431/133‑1 (MQ=255)
                          gAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAggg                                                                                                                      <  2:332944/149‑1 (MQ=255)
                          gAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAggg                                                                                                                      <  7:62085/149‑1 (MQ=255)
                           aaTCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAgggg                                                                                                                     >  3:131568/1‑149 (MQ=255)
                           aaTCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCAGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAgggg                                                                                                                     >  5:57501/1‑149 (MQ=255)
                               ggCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGaa                                                                                                                 <  8:170432/149‑1 (MQ=255)
                                 cAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGt                                                                                                               >  7:84864/1‑149 (MQ=255)
                                 cAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGt                                                                                                               <  8:116908/149‑1 (MQ=255)
                                                  ccTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAg                                                                                              >  4:16673/1‑149 (MQ=255)
                                                        gCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCAtt                                                                                        >  4:48050/1‑149 (MQ=255)
                                                        gCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCAtt                                                                                        <  4:131568/149‑1 (MQ=255)
                                                          aGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTc                                                                                      <  5:1316/149‑1 (MQ=255)
                                                                  ggCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCAt                                                                                         <  4:160690/138‑1 (MQ=255)
                                                                  ggCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCAt                                                                                         >  3:160690/1‑138 (MQ=255)
                                                                  ggCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTc                                                                                <  6:260088/147‑1 (MQ=255)
                                                                     aaaCGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGGGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCCTTTCCCCCGCGCCCt                                                                           >  6:327775/1‑149 (MQ=255)
                                                                                cAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTcc                                                                <  4:359162/149‑1 (MQ=255)
                                                                                   tCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCggg                                                             <  4:185816/149‑1 (MQ=255)
                                                                                   tCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCggg                                                             >  6:9210/1‑149 (MQ=255)
                                                                                      cGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTaa                                                          <  2:272969/149‑1 (MQ=255)
                                                                                      cGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTaa                                                          >  1:272969/1‑149 (MQ=255)
                                                                                           ccGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGt                                                      <  8:84864/148‑1 (MQ=255)
                                                                                            cGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTgg                                                    <  2:9405/149‑1 (MQ=255)
                                                                                              gTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAg                                                                    <  1:198956/131‑1 (MQ=255)
                                                                                              gTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAg                                                                    >  2:198956/1‑131 (MQ=255)
                                                                                                 gtgtTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGctct                                                <  3:16673/148‑1 (MQ=255)
                                                                                                       ccccGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGc                                          >  6:131759/1‑148 (MQ=255)
                                                                                                        cccgcccgTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGa                                        >  4:149653/1‑149 (MQ=255)
                                                                                                         ccgcccgTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGAt                                       >  7:302671/1‑149 (MQ=255)
                                                                                                                      ccAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCggg                                                             >  4:361910/1‑114 (MQ=255)
                                                                                                                      ccAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCggg                                                             <  3:361910/114‑1 (MQ=255)
                                                                                                                       cAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCgg                                                              >  6:135528/1‑112 (MQ=255)
                                                                                                                       cAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCgg                                                              <  5:135528/112‑1 (MQ=255)
                                                                                                                                 caccaACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAgg               >  6:85671/1‑149 (MQ=255)
                                                                                                                                       ctgatgcgcCTGTTCGAGCCGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAggctggct         >  4:132262/6‑149 (MQ=255)
                                                                                                                                           ggCGCCGGCTCGCACAGGCGCATCAGGTGGGCAGGGGGGAAGTCGCTGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGAGCGGCCCAGCTGCGCACGAACCAGGCTGGCttgtt     <  8:347703/149‑1 (MQ=255)
                                                                                                                                              gcCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGCAGTCGATGCAGCCGTTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTc  <  4:30596/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCTCACACCGCTCATCGGCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTC  >  NC_002947/6001083‑6001373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑