Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 437,491 C→A 10.3% W290L (TGG→TTG)  rdoA ← serine/threonine protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947437,4910CA10.3% 170.4 / 9.5 58W290L (TGG→TTG) rdoAserine/threonine protein kinase
Reads supporting (aligned to +/- strand):  ref base C (24/28);  new base A (4/2);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.61e-01

TTCTAGAACAATTTCAGCGGAGCTTCATCCAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAGGCGCTCCTCGCGGTTGCCGGCCAGCATCATC  >  NC_002947/437350‑437637
                                                                                                                                             |                                                                                                                                                  
ttCTAGAACAATTTCAGCGGAGCTTCATCCAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGgcgc                                                                                                                                              >  8:266670/1‑148 (MQ=255)
    aGAACAATTTCAGCGGAGCTTCATCCAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAg                                                                                                                                         >  6:144116/1‑149 (MQ=255)
     gAACAATTTCAGCGGAGCTTCATCCAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCtata                                                                                                                                          <  6:134637/147‑1 (MQ=255)
     gAACAATTTCAGCGGAGCTTCATCCAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCtata                                                                                                                                          <  7:32239/147‑1 (MQ=255)
     gAACAATTTCAGCGGAGCTTCATCCAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCtata                                                                                                                                          <  5:279438/147‑1 (MQ=255)
     gAACAATTTCAGCGGAGCTTCATCCAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGt                                                                                                                                        <  6:170393/149‑1 (MQ=255)
        cAATTTCAGCGGAGCTTCATCCAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCa                                                                                                                                     <  8:337442/149‑1 (MQ=255)
               aGCGGAGCTTCATCCAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGgcg                                                                                                                              >  7:252728/1‑149 (MQ=255)
                   gAGCTTCATCCAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAgg                                                                                                                          <  7:266670/149‑1 (MQ=255)
                   gAGCTTCATCCAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAgg                                                                                                                          >  2:190920/1‑149 (MQ=255)
                            ccAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGgcgcg                                                                                                                     >  6:54424/1‑145 (MQ=255)
                            ccAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGgcgcg                                                                                                                     <  5:54424/145‑1 (MQ=255)
                            ccAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAgg                                                                                                                 >  5:147115/1‑149 (MQ=255)
                             cAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAgg                                                                                                                 <  1:190920/148‑1 (MQ=255)
                             cAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAgg                                                                                                                 <  6:2542/148‑1 (MQ=255)
                             cAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAgg                                                                                                                 <  2:91350/148‑1 (MQ=255)
                              aGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAgggg                                                                                                               >  4:113149/1‑149 (MQ=255)
                                cgcAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCt                                                                                                             <  5:159404/149‑1 (MQ=255)
                                     ccATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGAt                                                                                                         >  3:151659/1‑148 (MQ=255)
                                       aTCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAg                                                                                                      >  8:150639/1‑149 (MQ=255)
                                           gTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGc                                                                                                   >  5:94151/1‑148 (MQ=255)
                                            ttCGCGCAAGGCGAGTATCTGGTCGCCCGAGTAGCGTGGCTGGCCGAAGCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGc                                                                                                   <  6:63153/147‑1 (MQ=255)
                                            ttCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAgg                                                                                                     <  3:113149/145‑1 (MQ=255)
                                                cgcAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTc                                                                                             <  7:269840/149‑1 (MQ=255)
                                                   aaGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGACGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCCGCCCGGCGCTATAGTGCAGCTGGCGCCGGGCGCGCAGGGGCTCGATCAGGGCCAGGTcgcg                                                                                          >  8:267843/1‑149 (MQ=255)
                                                    aGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTcgcgc                                                                                         <  2:188471/149‑1 (MQ=255)
                                                            aTCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGaa                                                                                 <  8:252728/149‑1 (MQ=255)
                                                                 gTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGt                                                                               <  5:144116/146‑1 (MQ=255)
                                                                 gTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGt                                                                            <  4:117354/149‑1 (MQ=255)
                                                                   cGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGgcgcgc                                                                                                                    >  5:207756/1‑107 (MQ=255)
                                                                   cGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGgcgcgc                                                                                                                    <  6:207756/107‑1 (MQ=255)
                                                                   cGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTgg                                                                          <  7:176963/149‑1 (MQ=255)
                                                                    gCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCg                                                                                                           >  6:373395/1‑115 (MQ=255)
                                                                    gCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCg                                                                                                           <  5:373395/115‑1 (MQ=255)
                                                                      cccAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAgg                                                                                                                 >  6:280592/1‑107 (MQ=255)
                                                                      cccAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAgg                                                                                                                 <  5:280592/107‑1 (MQ=255)
                                                                      cccAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGaa                                                                                 >  5:106551/1‑139 (MQ=255)
                                                                      cccAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGaa                                                                                 <  6:106551/139‑1 (MQ=255)
                                                                        cAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTc                                                                     >  2:281592/1‑149 (MQ=255)
                                                                                  gctggcCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCg                                                           >  3:183323/1‑149 (MQ=255)
                                                                                   ctggcCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGt                                                          <  4:183323/149‑1 (MQ=255)
                                                                                        cGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATc                                                     <  6:94151/149‑1 (MQ=255)
                                                                                         gAACCAAGGGAAGCTGGGCGGGAACACCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCa                                                    >  8:229827/1‑149 (MQ=255)
                                                                                                        gggCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGACCTCGTTGTAGCCGTCGATCCGCTCGGCCAGTTGcc                                     >  8:158954/1‑149 (MQ=255)
                                                                                                                    cGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGTTCAGCTCGGCCAGTTGCCCCAGGCGctcct                         >  2:348370/1‑149 (MQ=255)
                                                                                                                           tcCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAGGCGCTCCTCGCGGtt                  <  7:150639/149‑1 (MQ=255)
                                                                                                                           tcCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAGGCGCTCCTCGCGGtt                  >  4:340126/1‑149 (MQ=255)
                                                                                                                           ctataGCGCCTGGCTGGCAAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAGGCGCTCCTCGCGGtt                  >  5:336228/5‑149 (MQ=255)
                                                                                                                            tataGCGCCTGGCTGGCAAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAGGCGctc                           >  7:330605/4‑139 (MQ=255)
                                                                                                                            tataGCGCCTGGCTGGCAAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAGGCGctc                           <  8:330605/136‑1 (MQ=255)
                                                                                                                            tataGCGCCTGGCTGGCAAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAGGCGCTCCTCGCGGTTg                 >  7:5516/4‑149 (MQ=255)
                                                                                                                             ataGCGCCTGGCTGGCAAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAgg                                <  5:333640/131‑1 (MQ=255)
                                                                                                                             ataGCGCCTGGCTGGCAAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTAGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAgg                                >  6:333640/3‑133 (MQ=255)
                                                                                                                               aGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGtt                                                                  >  8:130380/1‑97 (MQ=255)
                                                                                                                               aGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGtt                                                                  <  7:130380/97‑1 (MQ=255)
                                                                                                                                gcgcCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAGGCGCTCCTCGCGGTTGCCgg             >  8:38488/1‑149 (MQ=255)
                                                                                                                                   ccTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAg                                          <  1:202817/117‑1 (MQ=255)
                                                                                                                                   ccTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAg                                          >  2:202817/1‑117 (MQ=255)
                                                                                                                                        ccagccagGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAGGCGCTCCTCGCGGTTGCCGGCCAGcatc     <  6:147115/149‑1 (MQ=255)
                                                                                                                                            ccagGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAGGCGCTCCTCGCGGTTGCCGGCCAGCatcatc  >  8:227448/1‑148 (MQ=255)
                                                                                                                                            ccagGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAGGCGCTCCTCGCGGTTGCCGGCCAGCatcatc  >  8:196204/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
TTCTAGAACAATTTCAGCGGAGCTTCATCCAGCGCAGCCATCTGTTCGCGCAAGGCGAGAATCTGGTCGCCCCAGTAGCGTGGCTGGCCGAACCAAGGGAAGCTGGGCGGGAACGCCGGGTCGTCCCAGCGCCTTGCCAGCCAGGCGCTATAGTGCAGCTGGCGCAGGGCGCGCAGGGGCTCGATCAGGGCCAGTTCGCGCGGGTCGAAGTCGTGGAACTCGTTGTAGCCGTCGATCAGCTCGGCCAGTTGCCCCAGGCGCTCCTCGCGGTTGCCGGCCAGCATCATC  >  NC_002947/437350‑437637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑