Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,001,237 A→C 8.0% L355R (CTG→CGG)  amaA ← L‑pipecolate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,001,2370AC 8.0% 138.1 / 4.6 50L355R (CTG→CGG) amaAL‑pipecolate oxidase
Reads supporting (aligned to +/- strand):  ref base A (21/25);  new base C (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

CCGCTCATCGGCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAG  >  NC_002947/6001090‑6001385
                                                                                                                                                   |                                                                                                                                                    
ccGCTCATCGGCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAg                                                                                                                                                     <  2:42687/149‑1 (MQ=255)
       tCGGCGAAAACTGAATCGGCAGGCGGTCCGCCTGGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATAACGGTGCCGGTCGTGTTACCCCGACCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCTGTTCGAGCCGgcgcctg                                                                                                                                              <  7:164202/149‑4 (MQ=255)
       tCGGCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCAt                                                                                                                                               >  2:285895/1‑148 (MQ=255)
         ggCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAg                                                                                                                                            >  6:1316/1‑149 (MQ=255)
         ggCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAg                                                                                                                                            <  3:221581/149‑1 (MQ=255)
          gCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAgg                                                                                                                                           <  4:263114/149‑1 (MQ=255)
            gAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCACTCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAgggg                                                                                                                                         >  7:347703/1‑147 (MQ=255)
            gAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACCGTGCCGGTCGTCTTACCCCGCCCGTTGTACCCAGTCACCGCCACCACCCCAGGCGCCGGATCGAACAGGCGCATCAggtg                                                                                                                                         >  3:30596/1‑149 (MQ=255)
              aaaCGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAggtggt                                                                                                                                       <  6:300695/149‑1 (MQ=255)
               aaCGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAggtggt                                                                                                                                       >  1:9405/1‑148 (MQ=255)
                 cGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAg                                                                                                                                    <  6:352441/149‑1 (MQ=255)
                  ggAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCTGTTCGAGCCGgc                                                                                                                                                   >  1:190431/1‑133 (MQ=255)
                  ggAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCTGTTCGAGCCGgc                                                                                                                                                   <  2:190431/133‑1 (MQ=255)
                   gAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAggg                                                                                                                                  <  2:332944/149‑1 (MQ=255)
                   gAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAggg                                                                                                                                  <  7:62085/149‑1 (MQ=255)
                    aaTCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAgggg                                                                                                                                 >  3:131568/1‑149 (MQ=255)
                    aaTCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCAGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAgggg                                                                                                                                 >  5:57501/1‑149 (MQ=255)
                        ggCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGaa                                                                                                                             <  8:170432/149‑1 (MQ=255)
                          cAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGt                                                                                                                           >  7:84864/1‑149 (MQ=255)
                          cAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGt                                                                                                                           <  8:116908/149‑1 (MQ=255)
                                           ccTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAg                                                                                                          >  4:16673/1‑149 (MQ=255)
                                                 gCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCAtt                                                                                                    >  4:48050/1‑149 (MQ=255)
                                                 gCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCAtt                                                                                                    <  4:131568/149‑1 (MQ=255)
                                                   aGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTc                                                                                                  <  5:1316/149‑1 (MQ=255)
                                                           ggCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCAt                                                                                                     <  4:160690/138‑1 (MQ=255)
                                                           ggCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCAt                                                                                                     >  3:160690/1‑138 (MQ=255)
                                                           ggCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTc                                                                                            <  6:260088/147‑1 (MQ=255)
                                                              aaaCGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGGGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCCTTTCCCCCGCGCCCt                                                                                       >  6:327775/1‑149 (MQ=255)
                                                                         cAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTcc                                                                            <  4:359162/149‑1 (MQ=255)
                                                                            tCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCggg                                                                         <  4:185816/149‑1 (MQ=255)
                                                                            tCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCggg                                                                         >  6:9210/1‑149 (MQ=255)
                                                                               cGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTaa                                                                      <  2:272969/149‑1 (MQ=255)
                                                                               cGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTaa                                                                      >  1:272969/1‑149 (MQ=255)
                                                                                    ccGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGt                                                                  <  8:84864/148‑1 (MQ=255)
                                                                                     cGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTgg                                                                <  2:9405/149‑1 (MQ=255)
                                                                                       gTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAg                                                                                <  1:198956/131‑1 (MQ=255)
                                                                                       gTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAg                                                                                >  2:198956/1‑131 (MQ=255)
                                                                                          gtgtTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGctct                                                            <  3:16673/148‑1 (MQ=255)
                                                                                                ccccGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGc                                                      >  6:131759/1‑148 (MQ=255)
                                                                                                 cccgcccgTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGa                                                    >  4:149653/1‑149 (MQ=255)
                                                                                                  ccgcccgTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGAt                                                   >  7:302671/1‑149 (MQ=255)
                                                                                                               ccAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCggg                                                                         <  3:361910/114‑1 (MQ=255)
                                                                                                               ccAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCggg                                                                         >  4:361910/1‑114 (MQ=255)
                                                                                                                cAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCgg                                                                          <  5:135528/112‑1 (MQ=255)
                                                                                                                cAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCgg                                                                          >  6:135528/1‑112 (MQ=255)
                                                                                                                          caccaACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAgg                           >  6:85671/1‑149 (MQ=255)
                                                                                                                                ctgatgcgcCTGTTCGAGCCGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAggctggct                     >  4:132262/6‑149 (MQ=255)
                                                                                                                                    ggCGCCGGCTCGCACAGGCGCATCAGGTGGGCAGGGGGGAAGTCGCTGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGAGCGGCCCAGCTGCGCACGAACCAGGCTGGCttgtt                 <  8:347703/149‑1 (MQ=255)
                                                                                                                                       gcCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGCAGTCGATGCAGCCGTTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTc              <  4:30596/149‑1 (MQ=255)
                                                                                                                                                aaCAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGcc     <  5:131759/149‑1 (MQ=255)
                                                                                                                                                   aGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAg  <  6:57501/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGCTCATCGGCGAAAACGGAATCGGCAGGCTGTCCGCCTCGCCCTTGAGCAGGAACTCGGCAAACGCCCGGCCAATCACGGTGCCGGTCGTGTTACCCCGCCCGTTGTACCCAGTCACCGCCACCAACCCAGGCGCCGGCTCGAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAG  >  NC_002947/6001090‑6001385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑