Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,013,845 G→C 9.3% intergenic (+50/+60) xpt → / ← PP_5266 xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,013,8450GC 9.3% 115.4 / 4.2 43intergenic (+50/+60)xpt/PP_5266xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (20/19);  new base C (2/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01

GGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC  >  NC_002947/6013699‑6013977
                                                                                                                                                  |                                                                                                                                    
gggCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGtc                                                                                                                                    <  7:302947/149‑1 (MQ=255)
         tGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCt                                                                                                                           <  3:245814/149‑1 (MQ=255)
            cGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtgtg                                                                                                                        <  7:310983/149‑1 (MQ=255)
            cGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGGCTCGCACGCtgtg                                                                                                                        >  7:62173/1‑149 (MQ=255)
                      aGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCtgt                                                                                                              <  1:193308/149‑1 (MQ=25)
                        ccAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTTCCGTACCTGTgg                                                                                                            >  6:108235/1‑149 (MQ=255)
                        ccAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCACACACGTGCAGCACACGTCTCGAACGCTGTGCCGTAACAGTgg                                                                                                            >  7:287972/1‑149 (MQ=255)
                        ccAGGGGTACCGCGTAGAAGCGCTGGCCCGGGGGAAGTCGCTGGAAGGTGGTGGGGTCAGCGTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACCGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTgg                                                                                                            <  6:111966/149‑1 (MQ=18)
                          aGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCtgtg                                                                                                             <  5:243498/146‑1 (MQ=255)
                          aGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCtgtg                                                                                                             >  6:243498/1‑146 (MQ=255)
                             gTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCCCGCCCCCCCGGGCACCACAGGGGGGGAACGCGGTGGCGGAGCGGTGGGTGCg                                                                                                       >  4:147533/1‑149 (MQ=255)
                                   gcgTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCGCTACCACGGGTGCAGCAAAGGTCTCGAACGCTGTGCCGTACCGGTGGGAGCGGGCGTg                                                                                                 >  7:195921/1‑149 (MQ=255)
                                                  cccGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGc                                                                                  <  7:36418/149‑1 (MQ=255)
                                                   ccGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCAAGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtgtg                                                                                                                        <  3:117518/110‑1 (MQ=255)
                                                   ccGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCAAGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtgtg                                                                                                                        >  4:117518/1‑110 (MQ=255)
                                                             tCGCTGGAAGGTGGTGTGATCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCCCAGGGGCCGCAGAGGGCTGGGACCCGGTGCCCTACCTGTGGGGGCGGGCGTGCCCGCGATAGGGCGCAACCCGTTTAt                                                                       >  4:81417/1‑149 (MQ=25)
                                                               gCTGGATGGTGGTGTGATCAGCTTCATCGAATAACCGCGCCACCTGCGTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGTAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCt                                                                     <  3:81417/149‑1 (MQ=37)
                                                               gCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTAt                                                                       <  3:317302/147‑1 (MQ=255)
                                                                   gAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCACCACACGTCTCCAAAGCTGTGCCGTACCTGTGGGAGACGGCCTGCCCGCGAATGGCCGCAACCCAATAATCTGGtt                                                                 >  5:110391/1‑149 (MQ=37)
                                                                            gtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTg                                                                    <  1:108121/137‑1 (MQ=255)
                                                                             gggtCAGCTTCATCGAATACACGCGACACCTGCTGCGCGGGCCCGCCCGCTCCCACAGGTGCAGCACAGGGCGCGAACGCTGGGCCGTCCCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGcc                                                       <  5:306376/148‑1 (MQ=38)
                                                                                  aGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGcc                                                  <  3:147533/149‑1 (MQ=255)
                                                                                        aTCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaac                                            <  5:108235/149‑1 (MQ=255)
                                                                                        aTCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCCCACCCCCACGGGCAGCCCACGGAGGGGACGCGTTGGCGGACCGTGGGGGGCGGGGGGGGCCGCCGATGGCCGCACACCTTTTATGTGGTTGCCGCCCACCCCGCcaacaac                                            >  6:90292/1‑149 (MQ=255)
                                                                                                acggcgACACCGGCTGCGGGGGCAGGCCCGCTCCCAATGGTGCAGCACCGGTCTCGACCGCTGTGCCGTACCTGTGGGAGGGGGGGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                    <  1:345767/146‑1 (MQ=255)
                                                                                                     gACACCGGCTCCGCGGGCACGCCCGCTCCCACCGGGCCCGCACAGGTCTCGACCGCGGTGCCGTACCTGGGGGTGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTaaa                               <  6:21221/149‑1 (MQ=37)
                                                                                                           tGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCGGTGGGAGCGGGCGGGCCCGCGAATGGCCGCAACCCGTTTATCTGGTTGCCGCCAGCCCCGCCAACCACCAGCGCTGGTAAAgttgtt                         >  8:190291/1‑149 (MQ=37)
                                                                                                             cTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttcttc                        <  7:190291/148‑1 (MQ=255)
                                                                                                                cgcgGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCttt                     <  8:287972/148‑1 (MQ=255)
                                                                                                                 gcgGGCACGCCCGCTCCCACAGGTGCAGCACAGGGCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCTAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTaa                   <  8:62173/149‑1 (MQ=255)
                                                                                                                  cgGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAg                  >  1:41887/1‑149 (MQ=255)
                                                                                                                   gggCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGc                 >  4:143639/1‑149 (MQ=255)
                                                                                                                         gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc           <  1:317220/149‑1 (MQ=255)
                                                                                                                           ccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCgg         >  6:269290/1‑149 (MQ=255)
                                                                                                                           ccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCgg         >  7:123264/1‑149 (MQ=255)
                                                                                                                           ccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTCCTTTAAGCCCCTGCgg         >  7:276765/1‑149 (MQ=255)
                                                                                                                                 ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc   >  3:2936/1‑149 (MQ=255)
                                                                                                                                 ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc   >  2:21976/1‑149 (MQ=255)
                                                                                                                                 ccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc   >  2:105440/1‑149 (MQ=21)
                                                                                                                                 caaaagttGCAGCAAAGGTCTCGAACGCTGTGCCGTAAATGTGGGAGCGGGAGTGCACGCGAATGGACGAAACAATTTTATCTGGTTGCAGCCAGACCCGCCAACACAAAGCGCTTGTAAAGTTCTTCTTTAAGCCACTGCGGATGcc    >  2:27821/8‑148 (MQ=255)
                                                                                                                                  cacaGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCACCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc   >  7:107403/1‑148 (MQ=21)
                                                                                                                                   acaGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc  >  6:152988/1‑148 (MQ=255)
                                                                                                                                             cacaGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTgg                                   <  2:296669/105‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                    
GGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC  >  NC_002947/6013699‑6013977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑