Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,013,852 A→G 8.7% intergenic (+57/+53) xpt → / ← PP_5266 xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,013,8520AG 8.7% 115.2 / 3.1 47intergenic (+57/+53)xpt/PP_5266xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base A (22/19);  new base G (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01

TGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCT  >  NC_002947/6013708‑6014000
                                                                                                                                                |                                                                                                                                                    
tGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCt                                                                                                                                                  <  3:245814/149‑1 (MQ=255)
   cGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtgtg                                                                                                                                               <  7:310983/149‑1 (MQ=255)
   cGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGGCTCGCACGCtgtg                                                                                                                                               >  7:62173/1‑149 (MQ=255)
             aGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCtgt                                                                                                                                     <  1:193308/149‑1 (MQ=25)
               ccAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTTCCGTACCTGTgg                                                                                                                                   >  6:108235/1‑149 (MQ=255)
               ccAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCACACACGTGCAGCACACGTCTCGAACGCTGTGCCGTAACAGTgg                                                                                                                                   >  7:287972/1‑149 (MQ=255)
               ccAGGGGTACCGCGTAGAAGCGCTGGCCCGGGGGAAGTCGCTGGAAGGTGGTGGGGTCAGCGTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACCGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTgg                                                                                                                                   <  6:111966/149‑1 (MQ=18)
                 aGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCtgtg                                                                                                                                    <  5:243498/146‑1 (MQ=255)
                 aGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCtgtg                                                                                                                                    >  6:243498/1‑146 (MQ=255)
                    gTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCCCGCCCCCCCGGGCACCACAGGGGGGGAACGCGGTGGCGGAGCGGTGGGTGCg                                                                                                                              >  4:147533/1‑149 (MQ=255)
                          gcgTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCGCTACCACGGGTGCAGCAAAGGTCTCGAACGCTGTGCCGTACCGGTGGGAGCGGGCGTg                                                                                                                        >  7:195921/1‑149 (MQ=255)
                                         cccGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGc                                                                                                         <  7:36418/149‑1 (MQ=255)
                                          ccGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCAAGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtgtg                                                                                                                                               <  3:117518/110‑1 (MQ=255)
                                          ccGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCAAGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtgtg                                                                                                                                               >  4:117518/1‑110 (MQ=255)
                                                    tCGCTGGAAGGTGGTGTGATCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCCCAGGGGCCGCAGAGGGCTGGGACCCGGTGCCCTACCTGTGGGGGCGGGCGTGCCCGCGATAGGGCGCAACCCGTTTAt                                                                                              >  4:81417/1‑149 (MQ=25)
                                                      gCTGGATGGTGGTGTGATCAGCTTCATCGAATAACCGCGCCACCTGCGTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGTAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCt                                                                                            <  3:81417/149‑1 (MQ=37)
                                                      gCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTAt                                                                                              <  3:317302/147‑1 (MQ=255)
                                                          gAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCACCACACGTCTCCAAAGCTGTGCCGTACCTGTGGGAGACGGCCTGCCCGCGAATGGCCGCAACCCAATAATCTGGtt                                                                                        >  5:110391/1‑149 (MQ=37)
                                                                   gtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTg                                                                                           <  1:108121/137‑1 (MQ=255)
                                                                    gggtCAGCTTCATCGAATACACGCGACACCTGCTGCGCGGGCCCGCCCGCTCCCACAGGTGCAGCACAGGGCGCGAACGCTGGGCCGTCCCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGcc                                                                              <  5:306376/148‑1 (MQ=38)
                                                                         aGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGcc                                                                         <  3:147533/149‑1 (MQ=255)
                                                                               aTCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaac                                                                   <  5:108235/149‑1 (MQ=255)
                                                                               aTCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCCCACCCCCACGGGCAGCCCACGGAGGGGACGCGTTGGCGGACCGTGGGGGGCGGGGGGGGCCGCCGATGGCCGCACACCTTTTATGTGGTTGCCGCCCACCCCGCcaacaac                                                                   >  6:90292/1‑149 (MQ=255)
                                                                                       acggcgACACCGGCTGCGGGGGCAGGCCCGCTCCCAATGGTGCAGCACCGGTCTCGACCGCTGTGCCGTACCTGTGGGAGGGGGGGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                           <  1:345767/146‑1 (MQ=255)
                                                                                            gACACCGGCTCCGCGGGCACGCCCGCTCCCACCGGGCCCGCACAGGTCTCGACCGCGGTGCCGTACCTGGGGGTGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTaaa                                                      <  6:21221/149‑1 (MQ=37)
                                                                                                  tGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCGGTGGGAGCGGGCGGGCCCGCGAATGGCCGCAACCCGTTTATCTGGTTGCCGCCAGCCCCGCCAACCACCAGCGCTGGTAAAgttgtt                                                >  8:190291/1‑149 (MQ=37)
                                                                                                    cTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttcttc                                               <  7:190291/148‑1 (MQ=255)
                                                                                                       cgcgGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCttt                                            <  8:287972/148‑1 (MQ=255)
                                                                                                        gcgGGCACGCCCGCTCCCACAGGTGCAGCACAGGGCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCTAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTaa                                          <  8:62173/149‑1 (MQ=255)
                                                                                                         cgGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAg                                         >  1:41887/1‑149 (MQ=255)
                                                                                                          gggCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGc                                        >  4:143639/1‑149 (MQ=255)
                                                                                                                gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                  <  1:317220/149‑1 (MQ=255)
                                                                                                                  ccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCgg                                >  6:269290/1‑149 (MQ=255)
                                                                                                                  ccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCgg                                >  7:123264/1‑149 (MQ=255)
                                                                                                                  ccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTCCTTTAAGCCCCTGCgg                                >  7:276765/1‑149 (MQ=255)
                                                                                                                        ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                          >  3:2936/1‑149 (MQ=255)
                                                                                                                        ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                          >  2:21976/1‑149 (MQ=255)
                                                                                                                        ccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                          >  2:105440/1‑149 (MQ=21)
                                                                                                                        caaaagttGCAGCAAAGGTCTCGAACGCTGTGCCGTAAATGTGGGAGCGGGAGTGCACGCGAATGGACGAAACAATTTTATCTGGTTGCAGCCAGACCCGCCAACACAAAGCGCTTGTAAAGTTCTTCTTTAAGCCACTGCGGATGcc                           >  2:27821/8‑148 (MQ=255)
                                                                                                                         cacaGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCACCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                          >  7:107403/1‑148 (MQ=21)
                                                                                                                          acaGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc                         >  6:152988/1‑148 (MQ=255)
                                                                                                                                    cacaGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTgg                                                          <  2:296669/105‑1 (MQ=255)
                                                                                                                                            tcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt      >  7:98292/1‑149 (MQ=255)
                                                                                                                                            tcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt      >  6:234558/1‑149 (MQ=255)
                                                                                                                                              gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt      <  3:143639/147‑1 (MQ=255)
                                                                                                                                              gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt      <  8:195921/147‑1 (MQ=255)
                                                                                                                                              gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTg     <  5:90292/148‑1 (MQ=255)
                                                                                                                                                aCGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTCCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCt  <  8:276765/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCT  >  NC_002947/6013708‑6014000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: