Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,013,863 T→C 8.0% intergenic (+68/+42) xpt → / ← PP_5266 xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,013,8630TC 8.0% 132.9 / 3.0 51intergenic (+68/+42)xpt/PP_5266xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (20/25);  new base C (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.47e-01

AGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACC  >  NC_002947/6013721‑6014008
                                                                                                                                              |                                                                                                                                                 
aGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCtgt                                                                                                                                             <  1:193308/149‑1 (MQ=25)
  ccAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTTCCGTACCTGTgg                                                                                                                                           >  6:108235/1‑149 (MQ=255)
  ccAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCACACACGTGCAGCACACGTCTCGAACGCTGTGCCGTAACAGTgg                                                                                                                                           >  7:287972/1‑149 (MQ=255)
  ccAGGGGTACCGCGTAGAAGCGCTGGCCCGGGGGAAGTCGCTGGAAGGTGGTGGGGTCAGCGTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACCGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTgg                                                                                                                                           <  6:111966/149‑1 (MQ=18)
    aGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCtgtg                                                                                                                                            >  6:243498/1‑146 (MQ=255)
    aGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCtgtg                                                                                                                                            <  5:243498/146‑1 (MQ=255)
       gTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCCCGCCCCCCCGGGCACCACAGGGGGGGAACGCGGTGGCGGAGCGGTGGGTGCg                                                                                                                                      >  4:147533/1‑149 (MQ=255)
             gcgTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCGCTACCACGGGTGCAGCAAAGGTCTCGAACGCTGTGCCGTACCGGTGGGAGCGGGCGTg                                                                                                                                >  7:195921/1‑149 (MQ=255)
                            cccGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGc                                                                                                                 <  7:36418/149‑1 (MQ=255)
                                       tCGCTGGAAGGTGGTGTGATCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCCCAGGGGCCGCAGAGGGCTGGGACCCGGTGCCCTACCTGTGGGGGCGGGCGTGCCCGCGATAGGGCGCAACCCGTTTAt                                                                                                      >  4:81417/1‑149 (MQ=25)
                                         gCTGGATGGTGGTGTGATCAGCTTCATCGAATAACCGCGCCACCTGCGTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGTAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCt                                                                                                    <  3:81417/149‑1 (MQ=37)
                                         gCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTAt                                                                                                      <  3:317302/147‑1 (MQ=255)
                                             gAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCACCACACGTCTCCAAAGCTGTGCCGTACCTGTGGGAGACGGCCTGCCCGCGAATGGCCGCAACCCAATAATCTGGtt                                                                                                >  5:110391/1‑149 (MQ=37)
                                                      gtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTg                                                                                                   <  1:108121/137‑1 (MQ=255)
                                                       gggtCAGCTTCATCGAATACACGCGACACCTGCTGCGCGGGCCCGCCCGCTCCCACAGGTGCAGCACAGGGCGCGAACGCTGGGCCGTCCCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGcc                                                                                      <  5:306376/148‑1 (MQ=38)
                                                            aGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGcc                                                                                 <  3:147533/149‑1 (MQ=255)
                                                                  aTCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaac                                                                           <  5:108235/149‑1 (MQ=255)
                                                                  aTCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCCCACCCCCACGGGCAGCCCACGGAGGGGACGCGTTGGCGGACCGTGGGGGGCGGGGGGGGCCGCCGATGGCCGCACACCTTTTATGTGGTTGCCGCCCACCCCGCcaacaac                                                                           >  6:90292/1‑149 (MQ=255)
                                                                          acggcgACACCGGCTGCGGGGGCAGGCCCGCTCCCAATGGTGCAGCACCGGTCTCGACCGCTGTGCCGTACCTGTGGGAGGGGGGGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                                   <  1:345767/146‑1 (MQ=255)
                                                                               gACACCGGCTCCGCGGGCACGCCCGCTCCCACCGGGCCCGCACAGGTCTCGACCGCGGTGCCGTACCTGGGGGTGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTaaa                                                              <  6:21221/149‑1 (MQ=37)
                                                                                     tGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCGGTGGGAGCGGGCGGGCCCGCGAATGGCCGCAACCCGTTTATCTGGTTGCCGCCAGCCCCGCCAACCACCAGCGCTGGTAAAgttgtt                                                        >  8:190291/1‑149 (MQ=37)
                                                                                       cTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttcttc                                                       <  7:190291/148‑1 (MQ=255)
                                                                                          cgcgGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCttt                                                    <  8:287972/148‑1 (MQ=255)
                                                                                           gcgGGCACGCCCGCTCCCACAGGTGCAGCACAGGGCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCTAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTaa                                                  <  8:62173/149‑1 (MQ=255)
                                                                                            cgGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAg                                                 >  1:41887/1‑149 (MQ=255)
                                                                                             gggCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGc                                                >  4:143639/1‑149 (MQ=255)
                                                                                                   gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                          <  1:317220/149‑1 (MQ=255)
                                                                                                     ccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCgg                                        >  7:123264/1‑149 (MQ=255)
                                                                                                     ccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCgg                                        >  6:269290/1‑149 (MQ=255)
                                                                                                     ccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTCCTTTAAGCCCCTGCgg                                        >  7:276765/1‑149 (MQ=255)
                                                                                                           ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                                  >  2:21976/1‑149 (MQ=255)
                                                                                                           ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                                  >  3:2936/1‑149 (MQ=255)
                                                                                                           ccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                                  >  2:105440/1‑149 (MQ=21)
                                                                                                           caaaagttGCAGCAAAGGTCTCGAACGCTGTGCCGTAAATGTGGGAGCGGGAGTGCACGCGAATGGACGAAACAATTTTATCTGGTTGCAGCCAGACCCGCCAACACAAAGCGCTTGTAAAGTTCTTCTTTAAGCCACTGCGGATGcc                                   >  2:27821/8‑148 (MQ=255)
                                                                                                            cacaGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCACCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                                  >  7:107403/1‑148 (MQ=21)
                                                                                                             acaGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc                                 >  6:152988/1‑148 (MQ=255)
                                                                                                                       cacaGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTgg                                                                  <  2:296669/105‑1 (MQ=255)
                                                                                                                               tcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt              >  6:234558/1‑149 (MQ=255)
                                                                                                                               tcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt              >  7:98292/1‑149 (MQ=255)
                                                                                                                                 gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt              <  8:195921/147‑1 (MQ=255)
                                                                                                                                 gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt              <  3:143639/147‑1 (MQ=255)
                                                                                                                                 gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTg             <  5:90292/148‑1 (MQ=255)
                                                                                                                                   aCGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTCCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCt          <  8:276765/149‑1 (MQ=255)
                                                                                                                                     gCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCttt        <  4:117540/149‑1 (MQ=255)
                                                                                                                                       tgtgCCGTACCTGTGGGAGCGGGCGTGCCCACGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCa      >  5:30667/1‑149 (MQ=255)
                                                                                                                                         tgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCa      <  5:152988/147‑1 (MQ=255)
                                                                                                                                         ggCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATa    <  7:286349/148‑1 (MQ=255)
                                                                                                                                           ccGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTc       <  1:343407/144‑1 (MQ=255)
                                                                                                                                           ccGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTc       >  2:343407/1‑144 (MQ=255)
                                                                                                                                           ccGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAcc  <  7:32975/149‑1 (MQ=255)
                                                                                                                                           ccGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAc   <  5:269290/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
AGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACC  >  NC_002947/6013721‑6014008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑