Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,025,835 T→A 7.7% L236H (CTC→CAC)  PP_5276 → phospholipase D family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,025,8350TA 7.7% 153.4 / 3.2 52L236H (CTC→CAC) PP_5276phospholipase D family protein
Reads supporting (aligned to +/- strand):  ref base T (20/28);  new base A (2/2);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CCTGGGCGACGAGTATTTCGATGCCGAGCCCAACCTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGATGGCCTATCAGTCGCAGCCGCGCCTGGAT  >  NC_002947/6025689‑6025968
                                                                                                                                                  |                                                                                                                                      
gcTGGGCGACGAGTATTTCGATGCCGAGCACAACCTCAACTTCACCGACTTCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTTGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCctct                                                                                                                                      <  6:51321/148‑1 (MQ=255)
ccTGGGCGACGAGTATTTCGATGCCGAGCCCAACCTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCctct                                                                                                                                      <  8:9093/149‑1 (MQ=255)
 cTGGGCGACGAGTATTTCGATGCCGAGCCCAACCTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTg                                                                                                                                     <  2:72229/149‑1 (MQ=255)
    ggCAACGAGTATTTCGAAGCCGAGCCCAACCTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTgg                                                                                                                                    <  4:9619/147‑1 (MQ=255)
        acgaGTATTTCGATGCCGAGCCCAACCTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCGCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGAc                                                                                                                              >  2:156717/1‑149 (MQ=255)
               tttCGATGCCGAGCCCAACCTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGAt                                                                                                                        <  3:352077/148‑1 (MQ=255)
               tttCGATGCCGAGCCCAACCTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATg                                                                                                                       >  4:249517/1‑149 (MQ=255)
               tttCGATGCCGAGCCCAACCTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATg                                                                                                                       <  2:153362/149‑1 (MQ=255)
                       ccGAGCCCAACCTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGa                                                                                                                <  1:351902/148‑1 (MQ=255)
                        cGAGCCCAACCTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCACTCAGACGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGAcc                                                                                                              >  7:339454/1‑149 (MQ=255)
                          aGCCCAACCTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGCGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTg                                                                                                            <  8:68448/149‑1 (MQ=255)
                              cAACCTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCgg‑g                                                                                                       <  2:35859/149‑1 (MQ=255)
                                 ccTCAACTTGACCGACATCGACCTGCTGGGCATGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTa                                                                                                    <  3:295839/149‑1 (MQ=255)
                                 ccTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGATTCGACCAGTACTGGAACAGCGACCTCATCCGGCCGATAACCGATTTCCTCTGTCAGGACCCGGATGCCAACGACCTGCgg‑ga                                                                                                      >  7:324343/1‑146 (MQ=255)
                                           aCCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑Gc                                                                                                      <  8:205142/137‑1 (MQ=255)
                                           aCCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑Gc                                                                                                      >  7:205142/1‑137 (MQ=255)
                                             cGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGcc                                                                                        >  2:294867/1‑149 (MQ=255)
                                              gACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCt                                                                                       <  1:156717/149‑1 (MQ=255)
                                                      ccTGCTGCGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGaa                                                                                   <  8:333497/145‑1 (MQ=255)
                                                       ctgctgGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTc                                                                              >  2:1727/1‑149 (MQ=255)
                                                        tgctgGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAgt                                                                                 <  6:64996/145‑1 (MQ=255)
                                                        tgctgGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAgt                                                                                 >  5:64996/1‑145 (MQ=255)
                                                                 tGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCaaa                                                                    <  5:354430/149‑1 (MQ=255)
                                                                    gCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGc                                                                  >  6:70078/1‑148 (MQ=255)
                                                                               gAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAgcg                                                      >  4:3573/1‑149 (MQ=255)
                                                                                    gcTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAgg                                                 >  4:344269/1‑149 (MQ=255)
                                                                                     cTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGc                                                >  8:304021/1‑149 (MQ=255)
                                                                                              aGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGAGCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGa                                       >  1:116927/1‑149 (MQ=255)
                                                                                              aGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAgg                                                 >  4:265684/1‑139 (MQ=255)
                                                                                              aGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAgg                                                 <  3:265684/139‑1 (MQ=255)
                                                                                                cTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGGTCCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGAc                                      <  1:294867/149‑1 (MQ=255)
                                                                                                cTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGAc                                                            >  1:144237/1‑126 (MQ=255)
                                                                                                cTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGAc                                                            <  2:144237/126‑1 (MQ=255)
                                                                                                      ccAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGa                               <  3:3573/149‑1 (MQ=255)
                                                                                                       cAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGAt                              <  7:151029/149‑1 (MQ=255)
                                                                                                            cTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGcc                                               >  4:91533/1‑127 (MQ=255)
                                                                                                            cTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGcc                                               <  3:91533/127‑1 (MQ=255)
                                                                                                            cTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGATGGcc                          <  3:344269/148‑1 (MQ=255)
                                                                                                             ttgAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGGATGACCGGGATGCCAACGACCTGCGG‑TATAGCCGCCAGCGACTGGAAGTGTGGCTGGCCAAAGCCAGTACCCATCGCAAGGCCATGTAGGACCGCTTTTTGGc                           <  4:44514/144‑1 (MQ=255)
                                                                                                             tGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCaa                                                   <  1:212424/122‑1 (MQ=255)
                                                                                                             tGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCaa                                                   >  2:212424/1‑122 (MQ=255)
                                                                                                             tGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATCACCCGGATGCCAACGCCCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGCACCCAGCGCACGGCCCTGTACGACCGCTTGATGGc                           >  3:44514/1‑146 (MQ=255)
                                                                                                             tGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCACTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGATGGCCTa                        >  5:201438/1‑149 (MQ=255)
                                                                                                             tGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCACTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGATGGCCTa                        >  5:196430/1‑149 (MQ=255)
                                                                                                               gAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGATGGCCTAt                       >  8:177144/1‑148 (MQ=255)
                                                                                                                  cAGCGCCCTCAGCCGGCCGATCACCGATTTCCACTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGATGGCCTATCAGt                   <  6:201438/149‑1 (MQ=255)
                                                                                                                  cAGCGCCCTCAGCCGGCCGATCACCGATTTCCACTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGATGGCCTATCAGt                   <  6:196430/149‑1 (MQ=255)
                                                                                                                   aGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGATGGCCTATCAGTc                  <  3:249517/149‑1 (MQ=255)
                                                                                                                          tCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGcc                                                                 >  6:350375/1‑95 (MQ=255)
                                                                                                                          tCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGcc                                                                 <  5:350375/95‑1 (MQ=255)
                                                                                                                             gccggccgATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGATGGCCTATCAGTCGCAGCCgcgc        <  7:304021/149‑1 (MQ=255)
                                                                                                                               cggccgATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGATGGCCTATCAGTCGCAGCCGCGCCt      <  5:70078/149‑1 (MQ=255)
                                                                                                                                   cgATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGATGGCCTATCAGTCGCAGCCGCGCCTGGAt  <  1:1727/149‑1 (MQ=255)
                                                                                                                                   cgATCAACGATTTACTAGTGCAGTAACCGGATGCCAACGACCGGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGGCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGATGGCCTATCAGTCGCAGCCGCGCCTTGAt  <  8:324343/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                      
CCTGGGCGACGAGTATTTCGATGCCGAGCCCAACCTCAACTTCACCGACATCGACCTGCTGGGCGTGGGCCCGGTAGCCGAGCAGCTGGGGCACAGCTTCGACCAGTACTGGAACAGCGCCCTCAGCCGGCCGATCACCGATTTCCTCTGGCATGACCCGGATGCCAACGACCTGCGG‑GCTAGCCGCCAGCGCCTGGAAGTGTCGCTGGCCAAAGCCAGGACCCAGCGCAAGGCCCTGTACGACCGCTTGATGGCCTATCAGTCGCAGCCGCGCCTGGAT  >  NC_002947/6025689‑6025968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑