| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,077,125 | T→G | 10.2% | K192Q (AAG→CAG) ‡ | PP_5329 ← | phosphate ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,077,125 | 0 | T | G | 10.2% | 171.3 / 11.2 | 59 | K192Q (AAG→CAG) ‡ | PP_5329 | phosphate ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (33/20); new base G (2/4); total (35/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01 | |||||||||||
GGTACGGTCTTGACGCTGGCGGTCTTGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATGGCGT > NC_002947/6076977‑6077268 | ggTACGGTCTTGACGCTGGCGGTCTTGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCtt < 2:105584/149‑1 (MQ=255) gACGCTGGCGGTCTTGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGc < 4:311676/146‑1 (MQ=255) aCGCTGGCGGTCTTGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTACTTCAAGGAAGAAGccct < 4:315711/148‑3 (MQ=255) aCGCTGGCGGTCTTGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTACTTCAAGGAAGAAGccct < 4:310464/148‑3 (MQ=255) ttGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATAc > 5:109363/1‑149 (MQ=255) ttGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATAc > 3:15874/1‑149 (MQ=255) aaCCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGa < 5:339432/148‑1 (MQ=255) aCCAATACCCGAGTAGCCGATGACGTTCTGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGGCAGGCTGTGCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACACCGCTTCTACCATCACGTAGCCTTAGGTGCCCGATACCGAGt > 2:298380/1‑149 (MQ=255) ccAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGtt > 7:12414/1‑149 (MQ=255) cAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTg > 8:99638/1‑149 (MQ=255) tACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCgg < 1:14536/149‑1 (MQ=255) gTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACa < 5:364485/149‑1 (MQ=255) ccGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTg > 5:32072/1‑149 (MQ=255) gATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGa > 6:113170/1‑149 (MQ=255) aTGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGAt < 8:12414/149‑1 (MQ=255) cGTTCAGCGTGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGc > 6:376221/1‑149 (MQ=255) cGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGc < 3:119501/149‑1 (MQ=255) tCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCttg > 5:240209/1‑149 (MQ=255) aGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCttgtt < 7:99638/149‑1 (MQ=255) aGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCttgtt < 4:15874/149‑1 (MQ=255) aGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCttgtt > 1:158739/1‑149 (MQ=255) gCGTGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCttgttg < 5:376221/149‑1 (MQ=255) tGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTACTTCAAGGAAGAAGccctgt > 4:316535/1‑91 (MQ=38) tGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTACTTCAAGGAAGAAGccctgt < 3:316535/95‑5 (MQ=38) tGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGGTAGGCTTGAAGTCGCCTTTGCACAGGGCTACTTCAAGGAAGAAGccctgt > 8:333103/1‑91 (MQ=37) tGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGCTAGGCTTGAAGTCGCCTTTGCACAGGGCTACTTCAAGGAAGAAGccctgt < 7:333103/95‑5 (MQ=37) aTCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGcc > 5:364821/1‑149 (MQ=255) aCTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGt > 2:178110/1‑148 (MQ=255) cACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCacac > 4:204886/1‑149 (MQ=255) aCGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACAcc > 4:170505/1‑149 (MQ=255) gACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTca > 6:78798/1‑143 (MQ=255) gACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTca < 5:78798/143‑1 (MQ=255) aGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAg < 5:113170/149‑1 (MQ=255) aGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCt < 1:178110/149‑1 (MQ=255) gTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGAt < 1:125365/138‑1 (MQ=255) gTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGAt > 2:125365/1‑138 (MQ=255) gTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGCCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGc > 5:101955/1‑149 (MQ=255) cTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTAGTTGGCCAGGTCGCCGGACACACCGACGTCACCCCAGCTCTAGATGTCGGCCTTGCCACCg > 6:223646/1‑148 (MQ=255) ttGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGca > 3:269537/1‑149 (MQ=255) gAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTc < 7:162452/124‑1 (MQ=255) gAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTc > 8:162452/1‑124 (MQ=255) aaGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGcaca < 6:364821/148‑1 (MQ=255) aGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAgg > 6:44510/1‑149 (MQ=255) aGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAgg > 8:5285/1‑149 (MQ=255) gTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGc > 3:56803/1‑149 (MQ=255) tCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAg > 8:254983/1‑146 (MQ=255) tCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAg < 7:254983/146‑1 (MQ=255) tCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGc > 5:257852/1‑148 (MQ=255) cGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGa < 3:204886/149‑1 (MQ=255) gCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAg > 2:272200/1‑149 (MQ=255) ccTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGt > 2:132458/1‑149 (MQ=255) cTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTg > 5:50439/1‑149 (MQ=255) cTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTg > 5:22241/1‑149 (MQ=255) acaGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTggag > 4:156253/1‑146 (MQ=255) ggCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGgaggag > 6:291397/1‑145 (MQ=255) gCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGAt < 6:109363/149‑1 (MQ=255) cttcttCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATg < 6:22241/149‑1 (MQ=255) cttcttCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATg > 8:141206/1‑149 (MQ=255) cttcCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATGGc < 1:132458/148‑1 (MQ=255) ttcCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATGGCGt > 8:84640/1‑149 (MQ=255) | GGTACGGTCTTGACGCTGGCGGTCTTGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATGGCGT > NC_002947/6076977‑6077268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |