Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,077,130 T→A 9.7% Y190F (TAC→TTC)  PP_5329 ← phosphate ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,077,1300TA 9.7% 174.4 / 8.3 62Y190F (TAC→TTC) PP_5329phosphate ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (33/23);  new base A (2/4);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

GACGCTGGCGGTCTTGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATGGCGTCGACCTGAGC  >  NC_002947/6076988‑6077278
                                                                                                                                              |                                                                                                                                                    
gACGCTGGCGGTCTTGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGc                                                                                                                                                   <  4:311676/146‑1 (MQ=255)
 aCGCTGGCGGTCTTGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTACTTCAAGGAAGAAGccct                                                                                                                                                <  4:315711/148‑3 (MQ=255)
 aCGCTGGCGGTCTTGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTACTTCAAGGAAGAAGccct                                                                                                                                                <  4:310464/148‑3 (MQ=255)
             ttGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATAc                                                                                                                                   >  5:109363/1‑149 (MQ=255)
             ttGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATAc                                                                                                                                   >  3:15874/1‑149 (MQ=255)
                 aaCCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGa                                                                                                                                <  5:339432/148‑1 (MQ=255)
                  aCCAATACCCGAGTAGCCGATGACGTTCTGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGGCAGGCTGTGCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACACCGCTTCTACCATCACGTAGCCTTAGGTGCCCGATACCGAGt                                                                                                                              >  2:298380/1‑149 (MQ=255)
                   ccAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGtt                                                                                                                             >  7:12414/1‑149 (MQ=255)
                    cAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTg                                                                                                                            >  8:99638/1‑149 (MQ=255)
                       tACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCgg                                                                                                                         <  1:14536/149‑1 (MQ=255)
                              gTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACa                                                                                                                  <  5:364485/149‑1 (MQ=255)
                                  ccGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTg                                                                                                              >  5:32072/1‑149 (MQ=255)
                                    gATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGa                                                                                                            >  6:113170/1‑149 (MQ=255)
                                     aTGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGAt                                                                                                           <  8:12414/149‑1 (MQ=255)
                                         cGTTCAGCGTGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGc                                                                                                       >  6:376221/1‑149 (MQ=255)
                                         cGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGc                                                                                                       <  3:119501/149‑1 (MQ=255)
                                            tCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCttg                                                                                                    >  5:240209/1‑149 (MQ=255)
                                              aGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCttgtt                                                                                                  >  1:158739/1‑149 (MQ=255)
                                              aGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCttgtt                                                                                                  <  4:15874/149‑1 (MQ=255)
                                              aGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCttgtt                                                                                                  <  7:99638/149‑1 (MQ=255)
                                               gCGTGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCttgttg                                                                                                 <  5:376221/149‑1 (MQ=255)
                                                        tGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTACTTCAAGGAAGAAGccctgt                                                                                                                                              <  3:316535/95‑5 (MQ=38)
                                                        tGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTACTTCAAGGAAGAAGccctgt                                                                                                                                              >  4:316535/1‑91 (MQ=38)
                                                        tGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGGTAGGCTTGAAGTCGCCTTTGCACAGGGCTACTTCAAGGAAGAAGccctgt                                                                                                                                              >  8:333103/1‑91 (MQ=37)
                                                        tGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGCTAGGCTTGAAGTCGCCTTTGCACAGGGCTACTTCAAGGAAGAAGccctgt                                                                                                                                              <  7:333103/95‑5 (MQ=37)
                                                          aTCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGcc                                                                                      >  5:364821/1‑149 (MQ=255)
                                                              aCTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGt                                                                                   >  2:178110/1‑148 (MQ=255)
                                                                  cACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCacac                                                                              >  4:204886/1‑149 (MQ=255)
                                                                   aCGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACAcc                                                                             >  4:170505/1‑149 (MQ=255)
                                                                     gACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTca                                                                                 <  5:78798/143‑1 (MQ=255)
                                                                     gACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTca                                                                                 >  6:78798/1‑143 (MQ=255)
                                                                                aGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAg                                                                <  5:113170/149‑1 (MQ=255)
                                                                                    aGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCt                                                            <  1:178110/149‑1 (MQ=255)
                                                                                                   gTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGAt                                                        <  1:125365/138‑1 (MQ=255)
                                                                                                   gTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGAt                                                        >  2:125365/1‑138 (MQ=255)
                                                                                                   gTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGCCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGc                                             >  5:101955/1‑149 (MQ=255)
                                                                                                         cTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTAGTTGGCCAGGTCGCCGGACACACCGACGTCACCCCAGCTCTAGATGTCGGCCTTGCCACCg                                        >  6:223646/1‑148 (MQ=255)
                                                                                                          ttGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGca                                      >  3:269537/1‑149 (MQ=255)
                                                                                                            gAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTc                                                             >  8:162452/1‑124 (MQ=255)
                                                                                                            gAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTc                                                             <  7:162452/124‑1 (MQ=255)
                                                                                                             aaGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGcaca                                    <  6:364821/148‑1 (MQ=255)
                                                                                                              aGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAgg                                  >  6:44510/1‑149 (MQ=255)
                                                                                                              aGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAgg                                  >  8:5285/1‑149 (MQ=255)
                                                                                                               gTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGc                                 >  3:56803/1‑149 (MQ=255)
                                                                                                                tCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAg                                   >  8:254983/1‑146 (MQ=255)
                                                                                                                tCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAg                                   <  7:254983/146‑1 (MQ=255)
                                                                                                                tCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGc                                 >  5:257852/1‑148 (MQ=255)
                                                                                                                 cGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGa                               <  3:204886/149‑1 (MQ=255)
                                                                                                                  gCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAg                              >  2:272200/1‑149 (MQ=255)
                                                                                                                   ccTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGt                             >  2:132458/1‑149 (MQ=255)
                                                                                                                    cTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTg                            >  5:50439/1‑149 (MQ=255)
                                                                                                                    cTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTg                            >  5:22241/1‑149 (MQ=255)
                                                                                                                          acaGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTggag                         >  4:156253/1‑146 (MQ=255)
                                                                                                                              ggCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGgaggag                      >  6:291397/1‑145 (MQ=255)
                                                                                                                               gCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGAt                 <  6:109363/149‑1 (MQ=255)
                                                                                                                                cttcttCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATg                <  6:22241/149‑1 (MQ=255)
                                                                                                                                cttcttCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATg                >  8:141206/1‑149 (MQ=255)
                                                                                                                                   cttcCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATGGc              <  1:132458/148‑1 (MQ=255)
                                                                                                                                    ttcCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATGGCGt            >  8:84640/1‑149 (MQ=255)
                                                                                                                                          gAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATGGCGTCGAc        <  5:266489/147‑1 (MQ=255)
                                                                                                                                          gAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATGGCGTCGAc        <  3:170505/147‑1 (MQ=255)
                                                                                                                                            aGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATGGCGTCGACCTGa    <  5:291397/149‑1 (MQ=255)
                                                                                                                                              tAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATGGCGTCGACCTGAGc  >  6:371801/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GACGCTGGCGGTCTTGTAACCAATACCCGAGTAGCCGATGCCGTTCAGCGAGGAGCTGATCGACTGCACGACCGAAGCCGAGCCAGGCTGTTCGTTGACGTTAGGCTTGAAGTCGCCTTTGCACAGGGCTTCTTCCTTGAAGTAGCCATAGGTGCCCGATACCGAGTTGCGGCCGAACAGCTGGATTGGCTTGTTGGCCAGGTCGCCGGTCACACCGACGTCACCCCAGGTCTTGATGTCGGCCTTGCCACCGCACAGGCGAGTGGAGGAGAAGATGGCGTCGACCTGAGC  >  NC_002947/6076988‑6077278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑