Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,110,033 T→A 5.2% D91V (GAT→GTT)  PP_5360 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,110,0330TA 5.2% 235.4 / 2.2 76D91V (GAT→GTT) PP_5360hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/39);  new base A (2/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CCTGGGCCGACGTAAGTGGTGAGCAAGCGCAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTC  >  NC_002947/6109886‑6110178
                                                                                                                                                   |                                                                                                                                                 
ccTGGGCCGACGTAAGTGGTGAGCAAGCGCAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATc                                                                                                                                                  <  5:323712/149‑1 (MQ=255)
 cTGGGCCGACGTAAGTGGTGAGCAAGCGCAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCa                                                                                                                                                 <  4:203428/149‑1 (MQ=255)
    ggCCGACGTAAGTGGTGAGCAAGCGCAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGc                                                                                                                                               <  7:112074/148‑1 (MQ=255)
            tAAGTGGTGAGCAAGCGCAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGc                                                                                                                                      >  8:290931/1‑149 (MQ=255)
            tAAGTGGTGAGCAAGCGCAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGc                                                                                                                                      <  7:126227/149‑1 (MQ=255)
              aGTGGTGAGCAAGCGCAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCt                                                                                                                                     <  5:292460/148‑1 (MQ=255)
                  gtgAGCAAGCGCAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATa                                                                                                                                <  5:149964/149‑1 (MQ=255)
                  gtgAGCAAGCGCAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATa                                                                                                                                >  3:245204/1‑149 (MQ=255)
                  gtgAGCAAGCGCAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGAGCAAACCAGACAGGTATAAGCCGCCACCAACCACGTAACATCATCTACGAAAGCTTCATa                                                                                                                                >  5:214701/1‑149 (MQ=255)
                      gCAAGCGCAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCa                                                                                                                            <  7:290931/149‑1 (MQ=255)
                        aaGCGCAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATg                                                                                                                          >  5:343195/1‑149 (MQ=255)
                            gcAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCaaa                                                                                                                      >  2:14862/1‑149 (MQ=255)
                             cAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAAt                                                                                                                     >  4:340580/1‑149 (MQ=255)
                              aaCGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGAACCGTCGTGCGCCAACCAGACAGGTATAAGCAGCCACCAACCACGTCACATCAGCTACGAAAGCTTCCTAACCATGCAAATc                                                                                                                    >  1:352903/1‑149 (MQ=255)
                               aCGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATcc                                                                                                                   >  7:340630/1‑149 (MQ=255)
                                  gTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATccgcc                                                                                                                >  3:3229/1‑149 (MQ=255)
                                     cGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCt                                                                                                             >  4:59547/1‑149 (MQ=255)
                                         aTCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCa                                                                                                          <  7:175392/148‑1 (MQ=255)
                                             acaTGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACgcag                                                                                                     >  3:231200/1‑149 (MQ=255)
                                                tGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACgcag                                                                                                     >  5:27433/1‑146 (MQ=255)
                                                    aGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCAACAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCt                                                                                              <  6:122752/149‑1 (MQ=255)
                                                      cGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTgg                                                                                            <  7:289968/149‑1 (MQ=255)
                                                      cGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTgg                                                                                            <  8:210277/149‑1 (MQ=255)
                                                          ggCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCaa                                                                                         <  1:14862/148‑1 (MQ=255)
                                                           gCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATa                                                                                       >  6:147190/1‑149 (MQ=255)
                                                                     tcGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGTCAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTg                                                                             <  6:214701/148‑1 (MQ=255)
                                                                         tCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGctcc                                                                         >  6:88827/1‑149 (MQ=255)
                                                                           aaCACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCACATCTCGCAATACCGGCTTCTGatactc                                                                       >  6:361735/1‑149 (MQ=255)
                                                                             cacGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGt                                                                     <  8:340630/149‑1 (MQ=255)
                                                                             cacGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGt                                                                     >  1:126606/1‑149 (MQ=255)
                                                                               cgcgCAAGCGCAAACCGACCCGTCGTGCGCCATCCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTc                                                                   <  5:361735/149‑1 (MQ=255)
                                                                               cgcgCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTc                                                                   <  8:109515/149‑1 (MQ=255)
                                                                                tcgcaagcgcaaACCGACCCGTCGTGAGCCAACCAGACATGTATAAGACGCAACCAACCACGTAACATCAGCTACGAAAGCTTCATACACATGCAAATCCGCCGCTTCACGCAGCAGATCTTGCAATACCTGCTTCTGCTCCTCGtt                                                                    <  4:45078/146‑1 (MQ=255)
                                                                                gcgcaagcgcaaACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGtt                                                                    >  3:45078/1‑147 (MQ=255)
                                                                                      gcgcaaACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACatcaa                                                            >  4:11340/1‑149 (MQ=255)
                                                                                             ccGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCg                                                     <  6:27433/149‑1 (MQ=255)
                                                                                              cGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGc                                                    >  3:99216/1‑149 (MQ=255)
                                                                                                aCCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCt                                                   >  2:211064/1‑148 (MQ=255)
                                                                                                  cagtcgaGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCATCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCATCTGCTCCTCGTACACATAAATAACCCGCTGa                                                 >  8:35728/3‑148 (MQ=255)
                                                                                                   cgtcgtGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATc                                               <  3:11340/149‑1 (MQ=255)
                                                                                                     tcgtGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACAACAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAg                                             >  3:289641/1‑149 (MQ=255)
                                                                                                     tcgtGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACAACAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAg                                             >  3:294703/1‑149 (MQ=255)
                                                                                                          cgcCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCaa                                         <  6:343195/148‑1 (MQ=255)
                                                                                                           gcCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAgg                                       <  7:233040/149‑1 (MQ=255)
                                                                                                              aaCCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCt                                    >  4:168721/1‑149 (MQ=255)
                                                                                                               aCCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTg                                   >  5:192061/1‑149 (MQ=255)
                                                                                                               aCCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTg                                   <  5:88827/149‑1 (MQ=255)
                                                                                                                ccAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTgg                                  >  6:94181/1‑149 (MQ=255)
                                                                                                                  aGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGc                                 >  6:350779/1‑148 (MQ=255)
                                                                                                                     cAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAg                             <  4:3229/149‑1 (MQ=255)
                                                                                                                      aGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCTTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAgg                            <  2:352903/149‑1 (MQ=255)
                                                                                                                        gTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGtt                                                                    >  5:313283/1‑107 (MQ=255)
                                                                                                                        gTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGtt                                                                    <  6:313283/107‑1 (MQ=255)
                                                                                                                        gTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCt                          >  8:203521/1‑149 (MQ=255)
                                                                                                                        gTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCt                          <  3:340580/149‑1 (MQ=255)
                                                                                                                           taAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGAt                       <  3:146471/149‑1 (MQ=255)
                                                                                                                           taAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCATCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGAt                       <  3:151978/149‑1 (MQ=255)
                                                                                                                            aaGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGc                           >  8:146561/1‑144 (MQ=255)
                                                                                                                            aaGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGc                           <  7:146561/144‑1 (MQ=255)
                                                                                                                              gccgccACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCt                          >  3:274956/1‑143 (MQ=255)
                                                                                                                              gccgccACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCt                          <  4:274956/143‑1 (MQ=255)
                                                                                                                              gccgccACCAACCACGTCACAACAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCAc                    <  4:289641/149‑1 (MQ=255)
                                                                                                                              gccgccACCAACCACGTCACAACAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCAc                    <  4:294703/149‑1 (MQ=255)
                                                                                                                                      caaccaCGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCgg            >  2:357429/1‑149 (MQ=255)
                                                                                                                                       aaccaCGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCgg            >  8:42919/1‑148 (MQ=255)
                                                                                                                                          caCGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCaaa        <  5:350779/149‑1 (MQ=255)
                                                                                                                                          caCGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCaaa        <  2:126606/149‑1 (MQ=255)
                                                                                                                                           aCGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAg       <  5:147190/149‑1 (MQ=255)
                                                                                                                                             gTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGc           <  1:238707/143‑1 (MQ=255)
                                                                                                                                             gTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGc           >  2:238707/1‑143 (MQ=255)
                                                                                                                                             gTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGtc     <  1:211064/149‑1 (MQ=255)
                                                                                                                                                acaTCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGtcctc  <  7:35728/149‑1 (MQ=255)
                                                                                                                                                acaTCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGtcct   <  7:42919/148‑1 (MQ=255)
                                                                                                                                                acaTCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTAATCACCAGTGCGGCAAAGtcct   <  6:192061/148‑1 (MQ=255)
                                                                                                                                                  aTCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCaa                              >  8:318152/1‑119 (MQ=255)
                                                                                                                                                  aTCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCaa                              <  7:318152/119‑1 (MQ=255)
                                                                                                                                                   tCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCaaa        <  6:80631/140‑1 (MQ=255)
                                                                                                                                                   tCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCaaa        >  5:80631/1‑140 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCTGGGCCGACGTAAGTGGTGAGCAAGCGCAACGGTACGTTATCCACATGGAAGCGTGGGCACATCGGAGCGTTCAACACGCGCAAGCGCAAACCGACCCGTCGTGCGCCAACCAGACAGGTATAAGCCGCCACCAACCACGTCACATCAGCTACGAAAGCTTCATACCCATGCAAATCCGCCGCTTCACGCAGCAGATCTGGCAATACCGGCTTCTGCTCCTCGTTCACATCAATCACCCGCTGATCAGCCAAGGGCTGGCCAAGGCTGATCACCAGTGCGGCAAAGTCCTC  >  NC_002947/6109886‑6110178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑