| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 445,090 | T→C | 10.5% | intergenic (+63/‑225) | PP_0367 → / → PP_0368 | hypothetical protein/acyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 445,090 | 0 | T | C | 10.5% | 120.6 / 5.3 | 48 | intergenic (+63/‑225) | PP_0367/PP_0368 | hypothetical protein/acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (22/20); new base C (2/3); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-01 | |||||||||||
TGGGCAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACG > NC_002947/444942‑445237 | tGGGCAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGGTCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTCTTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGt < 1:76965/149‑1 (MQ=255) aGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATc < 7:269339/149‑1 (MQ=255) cGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCGCGAATGCGTACGAACAGa > 6:278511/1‑148 (MQ=255) gAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGCCACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCATGCCCGCGGATGCGTCCGCTCAGCcaacaaat > 5:280021/1‑147 (MQ=255) gCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAAtttt < 6:151498/149‑1 (MQ=255) gCTCGGCGCAAAGGTCGTCAACGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAAtttt < 6:15610/149‑1 (MQ=255) ggCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCt < 8:207338/149‑1 (MQ=255) ggCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCt > 6:302220/1‑149 (MQ=37) cgcAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCGTGGCCCCGAATGAGTCCGCTCAGACAACAAATTTGTGTgg > 2:279571/1‑149 (MQ=255) cgcAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCAGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTgg < 5:183617/149‑1 (MQ=255) gcAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTgat < 7:60047/149‑3 (MQ=25) aaGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCgg < 7:292475/149‑1 (MQ=255) cgtcAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCctc < 5:131458/149‑1 (MQ=255) gtcAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCctct < 1:334800/149‑1 (MQ=255) ggCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGGCCGCGAATGCGTCCCATAAGAAAACAATTTTTTATGGTGCGGCCTCTTagcg > 3:301257/1‑149 (MQ=255) gCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAATATTGTTGTCTGATCGGACGCATTCGCgg < 1:168365/149‑1 (MQ=18) gCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCgg < 5:302220/149‑1 (MQ=18) aTCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTcgcg > 7:121305/1‑146 (MQ=255) aTCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTcgcg < 8:121305/146‑1 (MQ=255) ccGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCGCGAATGCGTCCGATCAGACAACAATTTTGTGTGGTCGGGCCTCTTAGCGGG‑Caa > 5:375033/1‑149 (MQ=255) gaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCg < 2:332510/149‑1 (MQ=255) aaTGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCATTCCCCCGGAATCGTGCCCTCACACAACAAATTTTTAAGGTGGGGCCACTTAGCCGG‑CCAGcccccg > 7:2557/1‑146 (MQ=255) gCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCCCGAATTCGTCCGATAAGACAAAAATTTTTTCTGGGCGGGCCGATTAGCGGGCCAAGccgcg > 8:152349/1‑143 (MQ=255) ccGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCCGCGCCTGCCTTGTCGGCGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAgg < 1:258129/149‑1 (MQ=255) cTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATc > 3:20451/1‑73 (MQ=255) cTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATc < 4:20451/73‑1 (MQ=255) gAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCGTTACCCCGAAAACGGACCAAAAGACAAAAATTTTTTGTGGTAGGGGCGATAAGCGGG‑CAAAGCCCCGCCAACAAAGGTTTGTGgcatagcatgcaagat > 4:86640/1‑143 (MQ=255) aCTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCtt < 1:144749/97‑1 (MQ=255) aCTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCtt > 2:144749/1‑97 (MQ=255) aTCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCGCGAATGCGTCCGATCAGACAACAATTTTTTCTGGTCGGGCCTCTTAGCGGG‑CAAGCCCGCTCCGACAACGGTTTGTGGCATGGCATGACTGATTTGCCAtttttag > 7:93497/1‑147 (MQ=255) tCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGCGTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CTAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGt < 5:278511/149‑1 (MQ=255) cccTATTTCCGCCCCGGCCTTGTAGGAGCGGGCTTGCCCGCTAATGCGTCCTATCACACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTg < 4:50832/148‑1 (MQ=38) gcctgcctTGTAGGAGCGGGCTGGCCCGCTAATGCGTCCTATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCAc < 1:279571/149‑1 (MQ=255) ttcgCGGGCAAGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATcc > 7:246133/4‑149 (MQ=255) cGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa < 7:251288/149‑1 (MQ=255) gCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa > 7:89959/1‑146 (MQ=255) cccGCTAATGCGTCCGTTCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGc < 3:86640/149‑1 (MQ=255) cccGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGc > 5:212092/1‑149 (MQ=18) ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGAc > 2:4866/1‑92 (MQ=255) ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt > 1:278994/1‑149 (MQ=255) ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt > 2:243089/1‑149 (MQ=255) cgcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACaa > 8:232935/1‑70 (MQ=255) cgcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACaa < 7:232935/70‑1 (MQ=255) cgcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt > 2:13328/1‑148 (MQ=255) gcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt > 4:330305/1‑147 (MQ=255) aaTGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAg > 2:229941/1‑147 (MQ=255) aaTGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAg < 1:229941/147‑1 (MQ=255) gTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACg > 5:159226/1‑149 (MQ=255) gTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACg > 2:172231/1‑149 (MQ=255) tCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACg > 3:320823/1‑148 (MQ=255) | TGGGCAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACG > NC_002947/444942‑445237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |