| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 445,120 | G→A | 8.9% | intergenic (+93/‑195) | PP_0367 → / → PP_0368 | hypothetical protein/acyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 445,120 | 0 | G | A | 8.9% | 160.1 / 4.8 | 56 | intergenic (+93/‑195) | PP_0367/PP_0368 | hypothetical protein/acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (31/20); new base A (3/2); total (34/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01 | |||||||||||
CGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGTTTGATCGGCTT > NC_002947/444976‑445266 | cgtcAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCctc < 5:131458/149‑1 (MQ=255) gtcAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCctct < 1:334800/149‑1 (MQ=255) ggCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGGCCGCGAATGCGTCCCATAAGAAAACAATTTTTTATGGTGCGGCCTCTTagcg > 3:301257/1‑149 (MQ=255) gCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAATATTGTTGTCTGATCGGACGCATTCGCgg < 1:168365/149‑1 (MQ=18) gCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCgg < 5:302220/149‑1 (MQ=18) aTCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTcgcg < 8:121305/146‑1 (MQ=255) aTCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTcgcg > 7:121305/1‑146 (MQ=255) ccGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCGCGAATGCGTCCGATCAGACAACAATTTTGTGTGGTCGGGCCTCTTAGCGGG‑Caa > 5:375033/1‑149 (MQ=255) gaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCg < 2:332510/149‑1 (MQ=255) aaTGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCATTCCCCCGGAATCGTGCCCTCACACAACAAATTTTTAAGGTGGGGCCACTTAGCCGG‑CCAGcccccg > 7:2557/1‑146 (MQ=255) gCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCCCGAATTCGTCCGATAAGACAAAAATTTTTTCTGGGCGGGCCGATTAGCGGGCCAAGccgcg > 8:152349/1‑143 (MQ=255) ccGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCCGCGCCTGCCTTGTCGGCGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAgg < 1:258129/149‑1 (MQ=255) gAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCGTTACCCCGAAAACGGACCAAAAGACAAAAATTTTTTGTGGTAGGGGCGATAAGCGGG‑CAAAGCCCCGCCAACAAAGGTTTGTGgcatagcatgcaagat > 4:86640/1‑143 (MQ=255) aCTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCtt > 2:144749/1‑97 (MQ=255) aCTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCtt < 1:144749/97‑1 (MQ=255) aTCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCGCGAATGCGTCCGATCAGACAACAATTTTTTCTGGTCGGGCCTCTTAGCGGG‑CAAGCCCGCTCCGACAACGGTTTGTGGCATGGCATGACTGATTTGCCAtttttag > 7:93497/1‑147 (MQ=255) tCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGCGTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CTAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGt < 5:278511/149‑1 (MQ=255) cccTATTTCCGCCCCGGCCTTGTAGGAGCGGGCTTGCCCGCTAATGCGTCCTATCACACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTg < 4:50832/148‑1 (MQ=38) gcctgcctTGTAGGAGCGGGCTGGCCCGCTAATGCGTCCTATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCAc < 1:279571/149‑1 (MQ=255) ttcgCGGGCAAGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATcc > 7:246133/4‑149 (MQ=255) cGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa < 7:251288/149‑1 (MQ=255) gCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa > 7:89959/1‑146 (MQ=255) cccGCTAATGCGTCCGTTCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGc < 3:86640/149‑1 (MQ=255) cccGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGc > 5:212092/1‑149 (MQ=18) ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGAc > 2:4866/1‑92 (MQ=255) ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt > 2:243089/1‑149 (MQ=255) ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt > 1:278994/1‑149 (MQ=255) cgcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACaa < 7:232935/70‑1 (MQ=255) cgcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACaa > 8:232935/1‑70 (MQ=255) cgcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt > 2:13328/1‑148 (MQ=255) gcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt > 4:330305/1‑147 (MQ=255) aaTGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAg < 1:229941/147‑1 (MQ=255) aaTGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAg > 2:229941/1‑147 (MQ=255) gTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACg > 2:172231/1‑149 (MQ=255) gTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACg > 5:159226/1‑149 (MQ=255) tCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACg > 3:320823/1‑148 (MQ=255) ccGACCAGACAAAATTGTTGTCTGATCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGt > 5:316564/1‑148 (MQ=38) cAGACAAAATTGTTGTCTGATCGGACGCATTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGttt > 2:61283/1‑149 (MQ=37) acaacaATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCa > 3:152137/1‑148 (MQ=255) caacaaTTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCaaa < 6:159226/149‑1 (MQ=255) aacaaTTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCGCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACACAAAGCTGAGATAAACTTAACTTTCaaa > 8:288766/1‑148 (MQ=255) aacaaTTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTc > 5:21619/1‑145 (MQ=255) aacaaTTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTc < 6:21619/145‑1 (MQ=255) aacaaTTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCaaa > 3:134284/1‑148 (MQ=255) catttttGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaacaa < 6:280021/146‑1 (MQ=255) aattgttGTCTGATCGGACGCATTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGTCAGAAAGCTGAGCTAAACGTAAGTTTCAaaca > 5:242786/6‑148 (MQ=37) aaTTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaacaac < 8:93497/149‑1 (MQ=255) tGTCTGATCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGtt < 6:316564/148‑1 (MQ=255) tCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGGATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGTTTgg > 2:314780/1‑148 (MQ=255) tGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGTTTGATc > 2:143074/1‑149 (MQ=255) tGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGTTTGATc > 5:32579/1‑149 (MQ=255) tGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGTTTGATc > 3:50028/1‑149 (MQ=255) gTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGttt < 4:350281/130‑1 (MQ=255) gTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGttt > 3:350281/1‑130 (MQ=255) gTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGTTTGATc < 1:172231/147‑1 (MQ=255) gTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGTTTGATCg > 2:304975/1‑148 (MQ=255) gggCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGTTTGATCGGCtt > 5:253688/1‑149 (MQ=255) | CGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGG‑CAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGTTTGATCGGCTT > NC_002947/444976‑445266 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |