Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 447,768 A→T 7.3% E116V (GAG→GTG)  PP_0369 → diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947447,7680AT 7.3% 236.7 / 7.4 82E116V (GAG→GTG) PP_0369diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (40/36);  new base T (3/3);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

ACGGTGTACATCGCCTACTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTGG  >  NC_002947/447623‑447916
                                                                                                                                                 |                                                                                                                                                    
aCGGTGTACATCGCCTACTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGc                                                                                                                                                    <  8:58245/148‑1 (MQ=255)
  ggTGTACATCGCCTACTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTa                                                                                                                                                 <  2:204653/149‑1 (MQ=255)
  ggTGTACATCGCCTACTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTa                                                                                                                                                 <  3:79715/149‑1 (MQ=255)
              cTACTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCtgctgc                                                                                                                                     <  1:366307/149‑1 (MQ=255)
               tACTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACctgctg                                                                                                                                      >  1:39942/1‑147 (MQ=255)
               tACTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACctgctg                                                                                                                                      >  3:329617/1‑147 (MQ=255)
                 cTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAgg                                                                                                                                  <  3:2245/149‑1 (MQ=255)
                   ttCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGAc                                                                                                                                >  6:709/1‑149 (MQ=255)
                     cTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGAcc                                                                                                                               <  3:227911/148‑1 (MQ=255)
                      tGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTg                                                                                                                             >  2:230131/1‑149 (MQ=255)
                         tCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGc                                                                                                                                                    >  5:210179/1‑123 (MQ=255)
                         tCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGc                                                                                                                                                    <  6:210179/123‑1 (MQ=255)
                          cGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGTGCGTACGCTCctgcca                                                                                                                                      <  6:323455/136‑3 (MQ=255)
                          cGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGTGCGTACGCTCctgcca                                                                                                                                      <  6:46460/136‑3 (MQ=255)
                          cGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGTGCGTACGCTCctgcca                                                                                                                                      >  5:323455/1‑134 (MQ=255)
                          cGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGTGCGTACGCTCctgcca                                                                                                                                      >  5:46460/1‑134 (MQ=255)
                          cGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACATTGTCTGCCTGTTGCTGCATGCGCTGTTCGGGCTGTATGGCGCCAGGCAGGAGCGTACGCAACTGCTGCAGGACATGGTCg                                                                                                                         >  4:208743/1‑149 (MQ=255)
                           gATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTCAGGAAGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGc                                                                                                                        >  3:134874/1‑149 (MQ=255)
                            aTACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGcc                                                                                                                       <  6:293801/149‑1 (MQ=255)
                                ccAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCcgcg                                                                                                                   >  3:301106/1‑149 (MQ=255)
                                ccAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCcgcg                                                                                                                   >  3:8609/1‑149 (MQ=255)
                                ccAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCcgcg                                                                                                                   >  6:179038/1‑149 (MQ=255)
                                ccAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCcgcg                                                                                                                   >  4:63150/1‑149 (MQ=255)
                                  aGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGTGCGTACGCTCctgcca                                                                                                                                      >  1:158373/1‑126 (MQ=255)
                                  aGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGTGCGTACGCTCctgcca                                                                                                                                      <  2:158373/128‑3 (MQ=255)
                                    aCCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCgcggtgc                                                                                                               >  7:80645/1‑149 (MQ=255)
                                     ccGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCgcggtgcg                                                                                                              <  3:20576/149‑1 (MQ=255)
                                      cGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCAc                                                                                                                                            <  6:142461/118‑1 (MQ=255)
                                      cGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCAc                                                                                                                                            >  5:142461/1‑118 (MQ=255)
                                      cGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTCGTCGCCCacgctgcgg                                                                                                             >  8:284676/1‑149 (MQ=255)
                                       gACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCgcggtgcggt                                                                                                            <  6:83077/149‑1 (MQ=255)
                                       gACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCgcggtgcggt                                                                                                            <  2:39942/149‑1 (MQ=255)
                                          ttCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCt                                                                                                         >  8:260546/1‑149 (MQ=255)
                                             tACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGtt                                                                                                      >  8:144929/1‑149 (MQ=255)
                                              aCCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGttt                                                                                                     <  1:230131/149‑1 (MQ=255)
                                               cggTGACGTTGGCCGCGTGAGTGAGGAGGCCGCGGTGATCTTCCAGCTTGTGTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGttt                                                                                                     <  7:284676/146‑1 (MQ=255)
                                                 gTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCAc                                                                                                                                            >  4:13821/1‑107 (MQ=255)
                                                 gTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCAc                                                                                                                                            <  3:13821/107‑1 (MQ=255)
                                                 gTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGcc                                                                                                  <  7:144929/149‑1 (MQ=255)
                                                   gACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGt                                                                                                <  3:63150/149‑1 (MQ=255)
                                                      cATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTggg                                                                                             >  2:242014/1‑149 (MQ=255)
                                                         ggCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAg                                                                                          >  2:119065/1‑149 (MQ=255)
                                                           ccGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAgtg                                                                                        <  4:329617/149‑1 (MQ=255)
                                                               gtgcgtgAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGc                                                                                    <  7:92048/149‑1 (MQ=255)
                                                                        gACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGcccg                                                                           >  1:45007/1‑148 (MQ=255)
                                                                        gACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCt                                                                           >  1:247779/1‑149 (MQ=255)
                                                                             cgcgGTGATCTTCCACGTTGTCTGCCTGTTGCTGCCGGAGCTGTTCGGGCTGTATGTCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCAGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAg                                                                       >  5:79361/1‑148 (MQ=255)
                                                                             cgcgGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGc                                                                      <  1:348816/149‑1 (MQ=255)
                                                                             cgcgGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGc                                                                      <  2:261592/149‑1 (MQ=255)
                                                                                    aTCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCt                                                                <  3:162372/148‑1 (MQ=255)
                                                                                     tCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTg                                                                                               >  1:313251/1‑116 (MQ=255)
                                                                                     tCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTg                                                                                               <  2:313251/116‑1 (MQ=255)
                                                                                       ttCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTAccc                                                            >  4:89860/1‑149 (MQ=255)
                                                                                           aCCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAg                                                                       <  1:167595/134‑1 (MQ=255)
                                                                                           aCCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAg                                                                       >  2:167595/1‑134 (MQ=255)
                                                                                                   tGCCTGTTGCTGCCGGCGCTGTTCGGGTTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCGCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAg                                                        >  1:118415/1‑141 (MQ=255)
                                                                                                   tGCCTGTTGCTGCCGGCGCTGTTCGGGTTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAg                                                        <  2:118415/141‑1 (MQ=255)
                                                                                                   tGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTAc                                                              <  8:227011/135‑1 (MQ=255)
                                                                                                   tGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTAc                                                              >  7:227011/1‑135 (MQ=255)
                                                                                                     ccTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGc                                                 <  4:301106/146‑1 (MQ=255)
                                                                                                         ttGCTGGCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCTGGACCTGGTGGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGGTGACCt                                           <  4:134874/148‑1 (MQ=255)
                                                                                                           gctgcCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTgg                                                                               >  3:124554/1‑110 (MQ=255)
                                                                                                           gctgcCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTgg                                                                               <  4:124554/110‑1 (MQ=255)
                                                                                                           gctgcCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGt                                        >  5:257258/1‑149 (MQ=255)
                                                                                                               ccGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGc                                    >  3:295133/1‑149 (MQ=255)
                                                                                                                 ggCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCg                                   >  4:178202/1‑148 (MQ=255)
                                                                                                                 ggCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTCCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCg                                   >  1:258995/1‑148 (MQ=255)
                                                                                                                  gcgcTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTTGTCGTTCGCTGTGTCGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGga                                 <  1:119065/149‑1 (MQ=255)
                                                                                                                    gcTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCg                                   >  7:229180/1‑145 (MQ=255)
                                                                                                                     cTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCg                                   >  6:327594/1‑144 (MQ=255)
                                                                                                                        ttCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGGGATCCg                           >  4:23579/1‑149 (MQ=255)
                                                                                                                        ttCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATcc                            <  2:247779/148‑1 (MQ=255)
                                                                                                                           ggtcTGTATGGCGGCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTTGGCAGTGTGGCTGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGTAGATCCGCt                         <  3:208743/145‑1 (MQ=255)
                                                                                                                             gCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGc                      >  8:54773/1‑149 (MQ=255)
                                                                                                                               tGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCg                    >  1:12984/1‑149 (MQ=255)
                                                                                                                                  aTGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCg                                   >  4:231092/1‑131 (MQ=255)
                                                                                                                                  aTGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCg                                   <  3:231092/131‑1 (MQ=255)
                                                                                                                                      cgcCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGTGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGc             <  7:260546/149‑1 (MQ=255)
                                                                                                                                       gcCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTg                        <  6:363981/137‑1 (MQ=255)
                                                                                                                                       gcCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTg                        >  5:363981/1‑137 (MQ=255)
                                                                                                                                         cTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGGGGCGGTGGCCCGCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATg          <  2:45007/149‑1 (MQ=255)
                                                                                                                                                gAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTg   >  6:147573/1‑149 (MQ=255)
                                                                                                                                                 aGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTgg  <  5:709/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ACGGTGTACATCGCCTACTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTGG  >  NC_002947/447623‑447916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑