Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 447,777 A→T 7.1% H119L (CAC→CTC)  PP_0369 → diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947447,7770AT 7.1% 245.5 / 7.2 85H119L (CAC→CTC) PP_0369diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (42/37);  new base T (3/3);  total (45/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01

CTACTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTGGATGAGCCT  >  NC_002947/447637‑447924
                                                                                                                                            |                                                                                                                                                   
cTACTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCtgctgc                                                                                                                                             <  1:366307/149‑1 (MQ=255)
 tACTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACctgctg                                                                                                                                              >  3:329617/1‑147 (MQ=255)
 tACTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACctgctg                                                                                                                                              >  1:39942/1‑147 (MQ=255)
   cTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAgg                                                                                                                                          <  3:2245/149‑1 (MQ=255)
     ttCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGAc                                                                                                                                        >  6:709/1‑149 (MQ=255)
       cTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGAcc                                                                                                                                       <  3:227911/148‑1 (MQ=255)
        tGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTg                                                                                                                                     >  2:230131/1‑149 (MQ=255)
            cGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGTGCGTACGCTCctgcca                                                                                                                                              >  5:46460/1‑134 (MQ=255)
            cGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGTGCGTACGCTCctgcca                                                                                                                                              <  6:323455/136‑3 (MQ=255)
            cGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGTGCGTACGCTCctgcca                                                                                                                                              <  6:46460/136‑3 (MQ=255)
            cGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGTGCGTACGCTCctgcca                                                                                                                                              >  5:323455/1‑134 (MQ=255)
            cGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACATTGTCTGCCTGTTGCTGCATGCGCTGTTCGGGCTGTATGGCGCCAGGCAGGAGCGTACGCAACTGCTGCAGGACATGGTCg                                                                                                                                 >  4:208743/1‑149 (MQ=255)
             gATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTCAGGAAGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGc                                                                                                                                >  3:134874/1‑149 (MQ=255)
              aTACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGcc                                                                                                                               <  6:293801/149‑1 (MQ=255)
                  ccAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCcgcg                                                                                                                           >  6:179038/1‑149 (MQ=255)
                  ccAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCcgcg                                                                                                                           >  3:8609/1‑149 (MQ=255)
                  ccAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCcgcg                                                                                                                           >  3:301106/1‑149 (MQ=255)
                  ccAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCcgcg                                                                                                                           >  4:63150/1‑149 (MQ=255)
                    aGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGTGCGTACGCTCctgcca                                                                                                                                              >  1:158373/1‑126 (MQ=255)
                    aGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGTGCGTACGCTCctgcca                                                                                                                                              <  2:158373/128‑3 (MQ=255)
                      aCCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCgcggtgc                                                                                                                       >  7:80645/1‑149 (MQ=255)
                       ccGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCgcggtgcg                                                                                                                      <  3:20576/149‑1 (MQ=255)
                        cGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCAc                                                                                                                                                    <  6:142461/118‑1 (MQ=255)
                        cGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCAc                                                                                                                                                    >  5:142461/1‑118 (MQ=255)
                        cGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTCGTCGCCCacgctgcgg                                                                                                                     >  8:284676/1‑149 (MQ=255)
                         gACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCgcggtgcggt                                                                                                                    <  6:83077/149‑1 (MQ=255)
                         gACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCgcggtgcggt                                                                                                                    <  2:39942/149‑1 (MQ=255)
                            ttCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCt                                                                                                                 >  8:260546/1‑149 (MQ=255)
                               tACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGtt                                                                                                              >  8:144929/1‑149 (MQ=255)
                                aCCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGttt                                                                                                             <  1:230131/149‑1 (MQ=255)
                                 cggTGACGTTGGCCGCGTGAGTGAGGAGGCCGCGGTGATCTTCCAGCTTGTGTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGttt                                                                                                             <  7:284676/146‑1 (MQ=255)
                                   gTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCAc                                                                                                                                                    <  3:13821/107‑1 (MQ=255)
                                   gTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCAc                                                                                                                                                    >  4:13821/1‑107 (MQ=255)
                                   gTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGcc                                                                                                          <  7:144929/149‑1 (MQ=255)
                                     gACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGt                                                                                                        <  3:63150/149‑1 (MQ=255)
                                        cATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTggg                                                                                                     >  2:242014/1‑149 (MQ=255)
                                           ggCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAg                                                                                                  >  2:119065/1‑149 (MQ=255)
                                             ccGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAgtg                                                                                                <  4:329617/149‑1 (MQ=255)
                                                 gtgcgtgAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGc                                                                                            <  7:92048/149‑1 (MQ=255)
                                                          gACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGcccg                                                                                   >  1:45007/1‑148 (MQ=255)
                                                          gACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCt                                                                                   >  1:247779/1‑149 (MQ=255)
                                                               cgcgGTGATCTTCCACGTTGTCTGCCTGTTGCTGCCGGAGCTGTTCGGGCTGTATGTCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCAGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAg                                                                               >  5:79361/1‑148 (MQ=255)
                                                               cgcgGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGc                                                                              <  2:261592/149‑1 (MQ=255)
                                                               cgcgGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGc                                                                              <  1:348816/149‑1 (MQ=255)
                                                                      aTCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCt                                                                        <  3:162372/148‑1 (MQ=255)
                                                                       tCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTg                                                                                                       >  1:313251/1‑116 (MQ=255)
                                                                       tCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTg                                                                                                       <  2:313251/116‑1 (MQ=255)
                                                                         ttCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTAccc                                                                    >  4:89860/1‑149 (MQ=255)
                                                                             aCCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAg                                                                               <  1:167595/134‑1 (MQ=255)
                                                                             aCCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAg                                                                               >  2:167595/1‑134 (MQ=255)
                                                                                     tGCCTGTTGCTGCCGGCGCTGTTCGGGTTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCGCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAg                                                                >  1:118415/1‑141 (MQ=255)
                                                                                     tGCCTGTTGCTGCCGGCGCTGTTCGGGTTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAg                                                                <  2:118415/141‑1 (MQ=255)
                                                                                     tGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTAc                                                                      >  7:227011/1‑135 (MQ=255)
                                                                                     tGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTAc                                                                      <  8:227011/135‑1 (MQ=255)
                                                                                       ccTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGc                                                         <  4:301106/146‑1 (MQ=255)
                                                                                           ttGCTGGCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCTGGACCTGGTGGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGGTGACCt                                                   <  4:134874/148‑1 (MQ=255)
                                                                                             gctgcCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTgg                                                                                       <  4:124554/110‑1 (MQ=255)
                                                                                             gctgcCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTgg                                                                                       >  3:124554/1‑110 (MQ=255)
                                                                                             gctgcCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGt                                                >  5:257258/1‑149 (MQ=255)
                                                                                                 ccGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGc                                            >  3:295133/1‑149 (MQ=255)
                                                                                                   ggCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCg                                           >  4:178202/1‑148 (MQ=255)
                                                                                                   ggCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTCCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCg                                           >  1:258995/1‑148 (MQ=255)
                                                                                                    gcgcTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTTGTCGTTCGCTGTGTCGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGga                                         <  1:119065/149‑1 (MQ=255)
                                                                                                      gcTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCg                                           >  7:229180/1‑145 (MQ=255)
                                                                                                       cTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCg                                           >  6:327594/1‑144 (MQ=255)
                                                                                                          ttCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGGGATCCg                                   >  4:23579/1‑149 (MQ=255)
                                                                                                          ttCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATcc                                    <  2:247779/148‑1 (MQ=255)
                                                                                                             ggtcTGTATGGCGGCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTTGGCAGTGTGGCTGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGTAGATCCGCt                                 <  3:208743/145‑1 (MQ=255)
                                                                                                               gCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGc                              >  8:54773/1‑149 (MQ=255)
                                                                                                                 tGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCg                            >  1:12984/1‑149 (MQ=255)
                                                                                                                    aTGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCg                                           >  4:231092/1‑131 (MQ=255)
                                                                                                                    aTGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCg                                           <  3:231092/131‑1 (MQ=255)
                                                                                                                        cgcCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGTGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGc                     <  7:260546/149‑1 (MQ=255)
                                                                                                                         gcCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTg                                >  5:363981/1‑137 (MQ=255)
                                                                                                                         gcCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTg                                <  6:363981/137‑1 (MQ=255)
                                                                                                                           cTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGGGGCGGTGGCCCGCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATg                  <  2:45007/149‑1 (MQ=255)
                                                                                                                                  gAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTg           >  6:147573/1‑149 (MQ=255)
                                                                                                                                   aGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTgg          <  5:709/149‑1 (MQ=255)
                                                                                                                                    gcctACGCACCTGCGGCAGGACCGGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTGGa         <  6:257258/146‑1 (MQ=255)
                                                                                                                                    gCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGc                     >  3:14885/1‑137 (MQ=255)
                                                                                                                                    gCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGc                     <  4:14885/137‑1 (MQ=255)
                                                                                                                                     cGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTGGAt        >  5:157483/1‑149 (MQ=255)
                                                                                                                                       tACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTGGAt        >  3:124343/1‑147 (MQ=255)
                                                                                                                                       tACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTGGATGa      <  2:48359/149‑1 (MQ=255)
                                                                                                                                       tACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTGGATGa      <  2:258995/149‑1 (MQ=255)
                                                                                                                                            aCCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTGGATGAGc    <  3:224760/146‑1 (MQ=255)
                                                                                                                                            aCCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTGGATGAGc    >  4:224760/1‑146 (MQ=255)
                                                                                                                                            aCCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTGGATGAGCCt  >  4:142501/1‑148 (MQ=255)
                                                                                                                                            aCCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTGGATGAGCCt  >  1:86765/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
CTACTTTCTGCTCGATACCCAGACCGACTTCTACCGTGACCATGGCCGCGTGCGTGAGGACGCCGCGGTGATCTTCCACCTTGTCTGCCTGTTGCTGCCGGCGCTGTTCGGGCTGTATGGCGCCTGGCAGGAGCGTACGCACCTGCTGCAGGACCTGGTCGCCCGCGGTGCGGTGCTGTTTGCCGTGGGCAGTGTGGCGGTGGCCCTCCAGCAAAGCTACCCCGAGGCGTTGCTGACCTGGTTGGCGGAGATCCGCTGGCCGGCTTTGCATGGCCACTGGATGAGCCT  >  NC_002947/447637‑447924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑