Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 524,352 G→C 8.1% intergenic (+219/‑597) tyrS → / → PP_16SC tyrosine‑‑tRNA ligase/16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947524,3520GC 8.1% 181.8 / 4.7 60intergenic (+219/‑597)tyrS/PP_16SCtyrosine‑‑tRNA ligase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (32/22);  new base C (3/2);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

GTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCC  >  NC_002947/524207‑524493
                                                                                                                                                 |                                                                                                                                             
gtTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCgcgggcg                                                                                                                                            <  4:333446/149‑1 (MQ=255)
    cTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCgcgggcg                                                                                                                                            <  8:234061/145‑1 (MQ=255)
    cTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGgcgc                                                                                                                                        >  6:369260/1‑149 (MQ=255)
    cTGTTGTAGGGGGAGGGGGCTGGTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCgcgggcg                                                                                                                                            >  7:234061/1‑145 (MQ=255)
         gtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGt                                                                                                                                   >  2:136159/1‑149 (MQ=255)
         gtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGCGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCCCGCGCGGCGCTCgg                                                                                                                                    <  7:91505/148‑1 (MQ=255)
            gggggagggggCTGTTTTGAGAGGCTGTTGGCGCTGAGATGTGTCCGCCACAAACCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACAGAGCGCCGCGCGGGCGGCGCTCGGtat                                                                                                                                 >  1:92571/1‑146 (MQ=255)
               ggagggggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATa                                                                                                                             >  4:94990/1‑149 (MQ=255)
                  gggggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGgc                                                                                                                          >  1:2704/1‑149 (MQ=255)
                     ggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCt                                                                                                                       <  1:342072/149‑1 (MQ=255)
                        tGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGa                                                                                                                     >  3:339410/1‑148 (MQ=255)
                          ttttGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAAc                                                                                                                  >  3:87981/1‑149 (MQ=255)
                                gagGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGt                                                                                                            >  2:361899/1‑149 (MQ=255)
                                  gggTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTgg                                                                                                          >  6:212694/1‑149 (MQ=255)
                                   ggTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTgg                                                                                                          <  8:158060/148‑1 (MQ=255)
                                   ggTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGc                                                                                                         <  3:247575/149‑1 (MQ=255)
                                               gagaTGTGTACGCCACAAACCTTGCAGAGCCTGTGAGATAGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACAGCCTATGAAACCGAGAGCCGCGCGGGCGGCGCGAGGGATCATAGGCTCTGAAACTTTCGTTGCGAAAACTTGGCa                                                                                             >  3:186355/1‑149 (MQ=255)
                                                agaTGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAg                                                                                            >  1:60881/1‑149 (MQ=255)
                                                    gtgtCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTc                                                                                        >  6:135689/1‑149 (MQ=255)
                                                          gCCACAACCCTTGCAGCGCCTGTGAGATAGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACg                                                                                  >  1:21117/1‑149 (MQ=255)
                                                            cacaACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACgg                                                                                 >  6:298481/1‑148 (MQ=255)
                                                            cacaACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGt                                                                                <  4:339410/149‑1 (MQ=255)
                                                            cacaACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGt                                                                                <  1:361899/149‑1 (MQ=255)
                                                            cacaACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGt                                                                                <  2:60881/149‑1 (MQ=255)
                                                             acaACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTc                                                                               >  8:203082/1‑149 (MQ=255)
                                                               aaCCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCtt                                                                             >  4:238178/1‑149 (MQ=255)
                                                                      gCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTccc                                                                        <  1:136159/147‑1 (MQ=255)
                                                                        aGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTta                                                                    <  5:317664/149‑1 (MQ=255)
                                                                             cTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATAttt                                                               >  5:212909/1‑149 (MQ=255)
                                                                                tgAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCa                                                             <  3:238178/148‑1 (MQ=255)
                                                                                  agaTCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTcccc                                                                       <  3:151687/136‑1 (MQ=255)
                                                                                  agaTCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTcccc                                                                       >  4:151687/1‑136 (MQ=255)
                                                                                  agaTCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACtt                                                          >  7:3478/1‑149 (MQ=255)
                                                                                       gAGCGCCGCCCGCGTCCGGCTTGAGACATTCCCCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCtt                                                      <  7:203082/148‑1 (MQ=255)
                                                                                       gAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCtt                                                      <  8:3478/148‑1 (MQ=255)
                                                                                       gAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCtt                                                      <  5:135689/148‑1 (MQ=255)
                                                                                               cccgcGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTaaa                                             <  3:94990/149‑1 (MQ=255)
                                                                                               cccgcGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTa                                               >  5:62671/1‑147 (MQ=255)
                                                                                                ccgcGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTaaa                                             <  5:267091/148‑1 (MQ=255)
                                                                                                ccgcGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTa                                               >  6:267091/1‑146 (MQ=255)
                                                                                                 cgcgTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGTGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAgg                                           <  5:212694/149‑1 (MQ=255)
                                                                                                 cgcgTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAgg                                           >  2:65856/1‑149 (MQ=255)
                                                                                                          cTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGccc                                  >  8:167380/1‑149 (MQ=255)
                                                                                                              agaCATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCtctc                              >  2:214060/1‑149 (MQ=255)
                                                                                                              agaCATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCtctc                              <  4:87981/149‑1 (MQ=255)
                                                                                                               gaCATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCtctc                              >  3:55176/1‑148 (MQ=255)
                                                                                                                         gCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTTGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTta                   <  5:298481/149‑1 (MQ=255)
                                                                                                                         gCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTta                   >  4:97266/1‑149 (MQ=255)
                                                                                                                           cTATGAGACCGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGGTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTtat                  >  2:160329/1‑148 (MQ=11)
                                                                                                                           cTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTtat                  >  5:148851/1‑148 (MQ=255)
                                                                                                                              tGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATcccc                      >  4:351205/1‑141 (MQ=255)
                                                                                                                              tGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATcccc                      <  3:351205/141‑1 (MQ=255)
                                                                                                                              tGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATaa                <  5:314811/147‑1 (MQ=255)
                                                                                                                              tGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATg              >  2:207717/1‑149 (MQ=255)
                                                                                                                                    cGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGccc         >  1:220256/1‑148 (MQ=255)
                                                                                                                                     gAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTGCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCa       >  2:159076/1‑149 (MQ=255)
                                                                                                                                     gAGCGCCGCCCGCGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATcc                        <  1:263859/132‑1 (MQ=21)
                                                                                                                                     gAGCGCCGCCCGCGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATcc                        >  2:263859/1‑132 (MQ=21)
                                                                                                                                      aGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCAc      <  1:214060/149‑1 (MQ=255)
                                                                                                                                         gccgcGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTc   >  7:151865/1‑149 (MQ=255)
                                                                                                                                         gccgcGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTc   <  6:62671/149‑1 (MQ=255)
                                                                                                                                          ccgcccgcGCGGCGCTCGGTTTCATAGGCGGTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTcc  <  3:77293/149‑1 (MQ=255)
                                                                                                                                           cgcgcgGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACg                                     >  8:15659/1‑113 (MQ=255)
                                                                                                                                           cgcgcgGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACg                                     <  7:15659/113‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCC  >  NC_002947/524207‑524493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑