Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 543,730 C→T 8.9% A657V (GCT→GTT)  rpoC → DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947543,7300CT 8.9% 286.0 / 12.7 102A657V (GCT→GTT) rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (45/47);  new base T (7/2);  total (53/49)
Fisher's exact test for biased strand distribution p-value = 1.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

Only 100 of 102 total aligned reads displayed.

TACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGA  >  NC_002947/543585‑543871
                                                                                                                                                    |                                                                                                                                             
tACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTAc                                                                                                                                            >  4:337897/1‑149 (MQ=255)
tACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTAc                                                                                                                                            >  1:287533/1‑149 (MQ=255)
  ccGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCAATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTAcc                                                                                                                                           >  3:299048/1‑148 (MQ=255)
    gcgTGGTTGGTCTGAAAGAGACCGTTATCTTCTCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCATTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGAt                                                                                                                                        <  2:40549/149‑1 (MQ=255)
     cgTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGAtg                                                                                                                                       <  5:177115/149‑1 (MQ=255)
     cgTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGAt                                                                                                                                        >  7:2534/1‑148 (MQ=255)
         gttggtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGAtgaagt                                                                                                                                   >  6:144447/1‑149 (MQ=255)
         gttggtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGAtgaag                                                                                                                                    <  3:346158/148‑1 (MQ=255)
          ttggtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGAtgaagt                                                                                                                                   >  7:263831/1‑148 (MQ=255)
             gtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGAtgaagtgaag                                                                                                                               <  2:226955/149‑1 (MQ=255)
             gtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGAtgaagtgaag                                                                                                                               <  1:349324/149‑1 (MQ=255)
              tCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTACATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCCACCGAtgaagtgaag                                                                                                                               >  6:340247/1‑148 (MQ=255)
                tGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGa                                                                                                                             >  4:202382/1‑148 (MQ=255)
                  aaaGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGaaa                                                                                                                           <  2:287533/148‑1 (MQ=255)
                      agaCCTTTATCTTCGCCGTCCAACTGCTTTACACCGGTTTCGCATAATCGACCATTTCCGGCGTTTCCATCTGTGTT‑AACGACTTCGTTATCCCGGACTAGA‑AAGCC‑CGCATCATCTGTAAAGCTAACGATGAAGTGAAGGAAATCgag                                                                                                                      <  2:28671/149‑1 (MQ=255)
                       gaCCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCgaga                                                                                                                     <  7:240009/149‑1 (MQ=255)
                        aCCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGAtgaagtgaag                                                                                                                               >  6:162738/1‑138 (MQ=255)
                        aCCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGAtgaagtgaag                                                                                                                               <  5:162738/138‑1 (MQ=255)
                          cGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGc                                                                                                                   >  1:326492/1‑148 (MQ=255)
                                   cGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTAc                                                                                                             <  4:201059/145‑1 (MQ=255)
                                      cGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGc                                                                                                           >  1:227624/1‑144 (MQ=255)
                                      cGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGc                                                                                                           <  2:227624/144‑1 (MQ=255)
                                       gACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAAATGAATACGTTATCCCGGACGAGA‑AACCC‑CGCATCATCGGTAACGATACCGATGAAGTCAAGGAAATCGAGAGCCATTACGCCTCCg                                                                                                      >  1:110422/1‑149 (MQ=255)
                                         ccAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGcc                                                                                                   >  5:275248/1‑149 (MQ=255)
                                           aaCTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTTCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCCACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTg                                                                                                 <  5:340247/149‑1 (MQ=255)
                                               gATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGAGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTaa                                                                                             >  1:124420/1‑149 (MQ=255)
                                                aTGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTaa                                                                                             <  2:177163/148‑1 (MQ=255)
                                                 tGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAAc                                                                                            <  3:337897/148‑1 (MQ=255)
                                                     cacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AATGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAgg                                                                                                                              >  1:44987/1‑110 (MQ=255)
                                                     cacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AATGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAgg                                                                                                                              <  2:44987/110‑1 (MQ=255)
                                                     cacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACTACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATAGAGAGCCAGTACGCATCCGGCCTTGt                                                                                               >  2:16342/1‑141 (MQ=255)
                                                     cacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGt                                                                                               <  1:16342/141‑1 (MQ=255)
                                                     cacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAgg                                                                                       <  5:144447/149‑1 (MQ=255)
                                                        cGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CACATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGc                                                                                     >  8:2141/1‑148 (MQ=255)
                                                          gTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCgag                                                                                  <  3:9298/149‑1 (MQ=255)
                                                           tttCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGAGGAGCC‑CGCATCATCGGTAACGCTACCGATTAAGTTAAGTAAATCGAGAGCCAGTACCCATCCGGCATGGTAACCCAgcgc                                                                                     >  1:294641/1‑146 (MQ=255)
                                                             tCGCCTACTCCACCACTTCCCGCGGTTCCATCGGTGTT‑AACGACCTCGTAATCCCGGCCGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGaa                                                                                <  8:263831/148‑1 (MQ=255)
                                                             tCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGaa                                                                                <  7:333233/148‑1 (MQ=255)
                                                               gCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTa                                                                             >  4:28197/1‑149 (MQ=255)
                                                                  tACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTacaa                                                                          <  6:275248/149‑1 (MQ=255)
                                                                   aCTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTacaa                                                                          >  1:195668/1‑148 (MQ=255)
                                                                    cTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTacaac                                                                         >  4:258338/1‑148 (MQ=255)
                                                                     tCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTaca                                                                           >  4:232778/1‑145 (MQ=255)
                                                                     tCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTaca                                                                           <  3:232778/145‑1 (MQ=255)
                                                                       gACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAgg                                                                     >  2:254772/1‑149 (MQ=255)
                                                                       gACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAgg                                                                     <  3:197192/149‑1 (MQ=255)
                                                                       gACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAg                                                                      <  2:124420/148‑1 (MQ=255)
                                                                       gACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CACATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAg                                                                      <  7:2141/148‑1 (MQ=255)
                                                                         ccATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGt                                                                    >  8:263231/1‑148 (MQ=255)
                                                                         ccATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTc                                                                   <  8:2534/149‑1 (MQ=255)
                                                                         ccATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTc                                                                   <  2:195668/149‑1 (MQ=255)
                                                                           aTTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGATA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCAt                                                                 <  3:262998/149‑1 (MQ=255)
                                                                            tttCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTacaa                                                                          <  3:226665/139‑1 (MQ=255)
                                                                            tttCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTacaa                                                                          >  4:226665/1‑139 (MQ=255)
                                                                               ccGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGAAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGa                                                              <  4:77381/148‑1 (MQ=255)
                                                                                  gCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTtg                                                          <  3:202382/149‑1 (MQ=255)
                                                                                         cATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCg                                                    >  6:297307/1‑148 (MQ=255)
                                                                                           tCGGTGTT‑AACGCCTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAg                                                 <  4:209682/149‑1 (MQ=255)
                                                                                           tCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAg                                                 <  2:326492/149‑1 (MQ=255)
                                                                                           tCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAg                                                 <  7:263231/149‑1 (MQ=255)
                                                                                                    aaagACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGATAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAacga                                         >  5:258381/4‑149 (MQ=255)
                                                                                                    aaCGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGAtgaagt                                                                                                                                   >  8:107913/1‑59 (MQ=255)
                                                                                                    aaCGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGTGCGAGaa                                                                                >  6:222428/1‑110 (MQ=255)
                                                                                                    aaCGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGaa                                                                                <  5:222428/110‑1 (MQ=255)
                                                                                                    aaCGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGaa                                                                                <  5:217159/110‑1 (MQ=255)
                                                                                                    aaCGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGaa                                                                                >  6:217159/1‑110 (MQ=255)
                                                                                                     aCGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAATGCGAGAGCCAGTACGCCTCCGGCCTGGTAACCGAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGTTCTAAGTCGCacgac                                        >  6:356374/1‑149 (MQ=255)
                                                                                                     aCGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTCCGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGCCTTGTGGTCGAAGGCGAacgac                                        >  3:84510/1‑149 (MQ=255)
                                                                                                       gACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAAcgacga                                      <  4:299048/149‑1 (MQ=255)
                                                                                                        aCTTCGTTATCCCGGACGAGA‑AAGCCGCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGaa                                                  >  1:190801/1‑137 (MQ=255)
                                                                                                        aCTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGaa                                                  <  2:190801/136‑1 (MQ=255)
                                                                                                          ttCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGAc                                                             <  7:306353/123‑1 (MQ=255)
                                                                                                          ttCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGAc                                                             >  8:306353/1‑123 (MQ=255)
                                                                                                          ttCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGAc                                                             >  8:311504/1‑123 (MQ=255)
                                                                                                          ttCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGAc                                                             <  7:311504/123‑1 (MQ=255)
                                                                                                            cGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAgt                                   <  8:191500/147‑1 (MQ=255)
                                                                                                              ttATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTc                                >  3:76382/1‑148 (MQ=255)
                                                                                                               tATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTcc                               >  2:219301/1‑148 (MQ=255)
                                                                                                                   ccGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGAAGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGc                          >  5:353173/1‑149 (MQ=255)
                                                                                                                    cGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGc                          >  2:268375/1‑148 (MQ=255)
                                                                                                                     ggACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCga                        >  1:95200/1‑149 (MQ=255)
                                                                                                                       aCGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCgatg                      <  4:84510/149‑1 (MQ=255)
                                                                                                                           ga‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATgg                  >  6:9023/1‑149 (MQ=255)
                                                                                                                           ga‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATgg                  <  2:95200/149‑1 (MQ=255)
                                                                                                                                 cttcacttcatcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCaa              >  3:181148/8‑149 (MQ=255)
                                                                                                                                 cc‑cGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAATGACGAAGTGTCCAAGGCGATGATGGCCAAc             <  1:219301/149‑1 (MQ=255)
                                                                                                                                  c‑cGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAAcc            >  1:322591/1‑149 (MQ=255)
                                                                                                                                    cacttcatcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATgg                  >  2:17388/5‑142 (MQ=255)
                                                                                                                                    cacttcatcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATgg                  <  1:17388/138‑1 (MQ=255)
                                                                                                                                      cttcatcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACt                                                            <  6:81474/96‑1 (MQ=255)
                                                                                                                                      cttcatcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACt                                                            >  5:81474/3‑98 (MQ=255)
                                                                                                                                      catcatCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACctct         <  3:28197/149‑1 (MQ=255)
                                                                                                                                       ttcatcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCGAAGGGGATGATGGCCACCctcgc        >  6:259366/2‑147 (MQ=255)
                                                                                                                                        tcatcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCtctc        >  4:107487/1‑148 (MQ=255)
                                                                                                                                         catcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCg       >  1:4413/1‑148 (MQ=255)
                                                                                                                                         catcGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCg       >  5:116002/1‑148 (MQ=255)
                                                                                                                                         catcGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCg       >  5:130067/1‑148 (MQ=255)
                                                                                                                                          atcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGa      >  3:226163/1‑148 (MQ=255)
                                                                                                                                           tcGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGaaa    <  2:110422/149‑1 (MQ=255)
                                                                                                                                             ggTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAaga  <  3:258338/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
TACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTT‑AACGACTTCGTTATCCCGGACGAGA‑AAGCC‑CGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGA  >  NC_002947/543585‑543871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑