| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 553,686 | C→T | 6.9% | R78C (CGC→TGC) | rpsS → | 30S ribosomal protein S19 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 553,686 | 0 | C | T | 6.9% | 172.6 / 2.2 | 58 | R78C (CGC→TGC) | rpsS | 30S ribosomal protein S19 |
| Reads supporting (aligned to +/- strand): ref base C (23/31); new base T (2/2); total (25/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01 | |||||||||||
GCCAGTTAAAACCTGGTCGCGCCGTTCGATGATCCTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGAAAGCCCGCTTGGTCGCCGACCAGATCCGCGGGAAGAAGGTGGGCG > NC_002947/553541‑553832 | gCCAGTTAAAACCTGGTCGCGCCGTTCGATGATCCTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCa < 5:149844/149‑1 (MQ=255)gCCAGTTAAAACCTGGGCGCGCCGTTCGATGATCCTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCa < 6:239841/149‑1 (MQ=255) ccAGTTAAAACCTGGTCGCGCCGTTCGATGATCCTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCAc < 2:112016/149‑1 (MQ=255) ttAAAACCTGGTCGCGCCGTTCGATGATCCTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTAccc > 7:156403/1‑141 (MQ=255) ttAAAACCTGGTCGCGCCGTTCGATGATCCTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTAccc < 8:156403/141‑1 (MQ=255) ccTGGTCGCGCCGTTCGATGATCCTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCggg > 4:261585/1‑149 (MQ=255) gcgcCGTTCGATGATCCTGCCACAAATGGTCGGTCTGACCATCGAGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTgg < 2:91164/149‑1 (MQ=255) gcCGTTCGATGATCCTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCAc > 1:98118/1‑130 (MQ=255) gcCGTTCGATGATCCTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCAc < 2:98118/130‑1 (MQ=255) tgatCCTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGaaa < 4:5574/149‑1 (MQ=255) tCCTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGcc < 1:314255/149‑1 (MQ=255) tCCTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGcc < 6:229463/149‑1 (MQ=255) cTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCaa < 2:275454/149‑1 (MQ=255) tGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAg > 5:155553/1‑149 (MQ=255) cgacaAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGc < 7:49588/146‑1 (MQ=255) cacaAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGt > 8:37599/1‑149 (MQ=255) aaTGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAg < 3:261585/149‑1 (MQ=255) ggtcggtcTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGa > 7:240539/1‑131 (MQ=255) ggtcggtcTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGa < 8:240539/131‑1 (MQ=255) gtcggtcTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAgggg < 6:165117/148‑1 (MQ=255) gtcTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAg > 5:289891/1‑148 (MQ=255) aCCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGaaa < 3:115484/147‑1 (MQ=255) cgGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAg > 7:183561/1‑149 (MQ=255) gTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGaa < 7:50460/143‑1 (MQ=255) gTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGaa > 8:50460/1‑143 (MQ=255) caACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTaa < 6:155553/149‑1 (MQ=255) aaCGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAg > 5:43990/1‑149 (MQ=255) aaCGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAg < 7:37599/149‑1 (MQ=255) cGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCaa < 3:69241/107‑1 (MQ=255) cGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCaa > 4:69241/1‑107 (MQ=255) cAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCCCGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCggga > 7:298412/1‑148 (MQ=255) aGCATGTTCCAGTTCTACTTAACGAAGACATGGTCGGCCACAAACTGGGCGAGATCGCGGGTACCCGCACCTATCCCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGgcg < 5:325031/149‑1 (MQ=255) ttCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCa > 1:162828/1‑149 (MQ=255) aGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGTGGTAAGGAAATGGAAGTAGCCGCTaa < 5:247462/128‑1 (MQ=255) aGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTaa > 6:247462/1‑128 (MQ=255) aGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCAtctc > 4:34087/1‑149 (MQ=255) gTTCTCGTGAACGAAGACATGGTCGGCCACAATCTGGGCGAGTTCGCCGGTACCTGCACCTTTCGCGGGCACGTGTCTGACAAGAAAGCCAAGCGTTAAGGGGTaa < 4:350988/106‑1 (MQ=255) gTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCTGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTaa < 8:11918/106‑1 (MQ=255) gTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCTGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTaa > 7:11918/1‑106 (MQ=255) gTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTAGACGGTACCTGCACCTATAGCGGGCACGTGGCTGACAAGAAAGACAAGCGTTAAGGGGTaa > 3:350988/1‑106 (MQ=255) gTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGcc < 6:289891/146‑1 (MQ=255) gTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGcc > 5:322959/1‑146 (MQ=255) gTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGcc > 6:215183/1‑146 (MQ=255) gTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGcc < 4:125720/146‑1 (MQ=255) aaCGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGaaa < 6:104682/149‑1 (MQ=255) aCGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGAAAg < 6:43990/149‑1 (MQ=255) cGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCgg < 7:340835/124‑1 (MQ=255) cGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCgg > 8:340835/1‑124 (MQ=255) gAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGAAAGcc > 6:212583/1‑149 (MQ=255) gACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGAAAGCCCGc < 2:162828/149‑1 (MQ=255) aCATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTGGCATCTCCGCCCAGAAAGCCCGCt > 7:111540/1‑149 (MQ=255) aCATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGAAAGCCCGCt < 3:34087/149‑1 (MQ=255) ggTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGaaa < 6:295565/138‑1 (MQ=255) ggTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGaaa > 5:295565/1‑138 (MQ=255) ccACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTaa < 4:68790/80‑1 (MQ=255) ccACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTaa > 3:68790/1‑80 (MQ=255) ccACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGAAAGCCCGCTTGGTCGCCGa < 8:183561/149‑1 (MQ=255) ttCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGAAAGCCCGCTTGGTCGCCGACCAGATCCGCggg > 8:100830/1‑146 (MQ=255) gCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGAAAGCCCGCTTGGTCGCCGACCAGATCCGCGGGaagaag > 7:275892/1‑149 (MQ=255) gCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGAAAGCCCGCTTGGTCGCCGACCAGATCCGCGGGaagaag < 5:215183/149‑1 (MQ=255) ccGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGAAAGCCCGCTTGGTCGCCGACCAGATCCGCGGGAAGAAGGTGGGCg > 8:317971/1‑148 (MQ=255) | GCCAGTTAAAACCTGGTCGCGCCGTTCGATGATCCTGCCACAAATGGTCGGTCTGACCATCGCGGTTCACAACGGTCGCCAGCATGTTCCAGTTCTCGTGAACGAAGACATGGTCGGCCACAAACTGGGCGAGTTCGCCGGTACCCGCACCTATCGCGGGCACGTGGCTGACAAGAAAGCCAAGCGTTAAGGGGTAAGGAAATGGAAGTAGCCGCTAAGTTGTCGGGCGCTCGCATCTCCGCCCAGAAAGCCCGCTTGGTCGCCGACCAGATCCGCGGGAAGAAGGTGGGCG > NC_002947/553541‑553832 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |