Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	563,783	T→C	100%	L289S (TTG→TCG)	rpoA →	DNA‑directed RNA polymerase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	563,783	0	T	C	100.0%	224.2 / NA	65	L289S (TTG→TCG)	rpoA	DNA‑directed RNA polymerase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/36);  total (29/36)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAGCAGGAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTTGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGCGACCGCCTGATCGTCG  >  NC_002947/563644‑563925                                                                                                                                              |                                                                                                                                               gAGCAGGAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTc                                                                                                                                        <  7:209640/148‑1 (MQ=255)  aGCAGGAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCt                                                                                                                                      <  4:246383/149‑1 (MQ=255)    cAGGAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTaa                                                                                                                                    >  1:348109/1‑149 (MQ=255)      ggAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTAGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAAcc                                                                                                                                  >  6:122264/1‑149 (MQ=255)       gAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGTAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCt                                                                                                                                 >  1:262480/1‑149 (MQ=255)       gAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCt                                                                                                                                 >  1:84328/1‑149 (MQ=255)        aaGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTACCCGAAG‑TAGAGCTGTCGAAGACTCCTAACCt                                                                                                                                 <  1:39508/149‑1 (MQ=255)         aGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTgg                                                                                                                               <  2:84328/149‑1 (MQ=255)         aGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTgg                                                                                                                               <  4:184454/149‑1 (MQ=255)         aGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTgg                                                                                                                               >  5:234352/1‑149 (MQ=255)         aGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTgg                                                                                                                               <  7:121421/149‑1 (MQ=255)         aGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTgg                                                                                                                               <  7:26310/149‑1 (MQ=255)         aGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTCACTGTACGTTCGGCCAACTGCATCAAGGCGGAGAACATCTACTACATCGGCGACCTGATACATCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCAgg                                                                                                                               >  1:164040/1‑149 (MQ=255)            cGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAGCTAGAGCTGTCGAAGACTCCTAACCTggg                                                                                                                              <  1:209405/148‑1 (MQ=255)             gagaTCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGt                                                                                                                             >  5:136001/1‑147 (MQ=255)             gagaTCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTaa                                                                                                                           <  2:177039/149‑1 (MQ=255)                atcgatcCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGTCGTGAGTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTaagaa                                                                                                                        >  2:285055/1‑149 (MQ=255)                 tcgatcCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTaagaa                                                                                                                        <  8:68176/148‑1 (MQ=255)                  cgatccgatcCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAAc                                                                                                                                   <  1:130419/136‑1 (MQ=255)                  cgatccgatcCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAAc                                                                                                                                   >  2:130419/1‑136 (MQ=255)                        gatccTGCTGCGTCCGGTTTTCGAGATGGAACTTACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGGGTA‑CCGAAG‑TAGAGCTGTCGAATACTCCTAACCTGGGTAAGAAGTCGctga                                                                                                                <  2:164040/149‑1 (MQ=255)                        gatccTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCctga                                                                                                                <  4:172197/149‑1 (MQ=255)                             tgctgcGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCctgactga                                                                                                            >  6:58836/1‑148 (MQ=255)                              gctgcGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGaaa                                                                                                          >  7:203656/1‑149 (MQ=255)                                  cGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATc                                                                                                        <  5:58836/147‑1 (MQ=255)                                   gTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAg                                                                                                     <  6:354716/149‑1 (MQ=255)                                      cGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGa                                                                                                   <  2:151183/148‑1 (MQ=255)                                       ggTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTc                                                                                                                                        <  6:110711/110‑1 (MQ=255)                                       ggTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTc                                                                                                                                        >  5:110711/1‑110 (MQ=255)                                        gTTGACCAGCTGGAACTGACTGTACGTTCGGACAACTGCCTCAATGCGGAGAACATCTACTACATCGGCGACCTGTTTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGt                                                                                                <  8:290704/149‑1 (MQ=255)                                            acgacCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTg                                                                                            <  2:348109/149‑1 (MQ=255)                                              gacCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGc                                                                                          >  1:145790/1‑149 (MQ=255)                                                ccTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGcc                                                                                         >  6:194899/1‑148 (MQ=255)                                                 cTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCctc                                                                                       >  4:133144/1‑149 (MQ=255)                                                      actgactgTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTg                                                                                  <  5:122264/149‑1 (MQ=255)                                                            tgTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGgtctgt                                                                            >  4:368119/1‑149 (MQ=255)                                                                      gCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCAt                                                                  >  3:270622/1‑149 (MQ=255)                                                                       ccAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATg                                                                 >  5:116812/1‑149 (MQ=255)                                                                               cTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGa                                                         <  8:203656/149‑1 (MQ=255)                                                                                  aaGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACaa                                                      <  1:129811/149‑1 (MQ=255)                                                                                  aaGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACaa                                                      <  1:134741/149‑1 (MQ=255)                                                                                  aaGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACaa                                                      >  5:43753/1‑149 (MQ=255)                                                                                        gagaACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGgcc                                                <  8:51467/149‑1 (MQ=255)                                                                                             cATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCt                                            >  5:74130/1‑148 (MQ=255)                                                                                             cATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCt                                            <  6:74130/148‑1 (MQ=255)                                                                                               tCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTg                                                                                            <  4:179711/98‑1 (MQ=255)                                                                                               tCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTg                                                                                            >  3:179711/1‑98 (MQ=255)                                                                                               tCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGgtctgt                                                                            >  1:93399/1‑114 (MQ=255)                                                                                               tCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGgtctgt                                                                            <  2:93399/114‑1 (MQ=255)                                                                                                      cATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTa                                  <  6:43753/149‑1 (MQ=255)                                                                                                       aTCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTaa                                 <  6:116812/149‑1 (MQ=255)                                                                                                       aTCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTaa                                 <  6:136001/149‑1 (MQ=255)                                                                                                        tCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCtt                                   >  2:167225/1‑146 (MQ=255)                                                                                                           gCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACATCTGGCCGCCTGCAAGt                                      <  8:140248/140‑1 (MQ=255)                                                                                                           gCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAGCTGGCCGCCTGCAAGt                                      >  7:140248/1‑140 (MQ=255)                                                                                                             gACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTaagaaaga                           >  4:27296/1‑149 (MQ=255)                                                                                                              aCCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAgac                          <  5:194899/149‑1 (MQ=255)                                                                                                                 tGATTCAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAgacga                        >  3:196652/1‑148 (MQ=255)                                                                                                                      cAGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAgg                   <  2:262480/148‑1 (MQ=255)                                                                                                                       aGCGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGTAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGc                  <  5:206271/148‑1 (MQ=255)                                                                                                                         cGTA‑CCGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGCGAc               <  1:285055/149‑1 (MQ=255)                                                                                                                               cGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGCGACCGCCt          <  6:234352/149‑1 (MQ=255)                                                                                                                               cGAAG‑TAGAGCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGCGACCGCCt          >  6:110310/1‑149 (MQ=255)                                                                                                                                      agagCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGACTTAAGAAAGACGACAAGGCGACCGCCTGAtcgt    >  5:43716/1‑149 (MQ=255)                                                                                                                                        agCTGTCGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGCGACCGCCTGAtcgtcg  >  1:253914/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                               GAGCAGGAAGACGAGATCGATCCGATCCTGCTGCGTCCGGTTGACGACCTGGAACTGACTGTACGTTCGGCCAACTGCCTCAAGGCGGAGAACATCTACTACATCGGCGACCTGATTCAGCGTA‑CCGAAG‑TAGAGCTGTTGAAGACTCCTAACCTGGGTAAGAAGTCCCTGACTGAAATCAAGGACGTTCTGGCCTCTCGTGGTCTGTCTCTCGGCATGCGCCTCGACAACTGGCCGCCTGCAAGTCTTAAGAAAGACGACAAGGCGACCGCCTGATCGTCG  >  NC_002947/563644‑563925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑