Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 571,617 G→A 7.5% intergenic (+43/+48) ssb → / ← PP_0486 single‑stranded DNA‑binding protein/GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947571,6170GA 7.5% 232.2 / 5.1 79intergenic (+43/+48)ssb/PP_0486single‑stranded DNA‑binding protein/GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (38/35);  new base A (4/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

GCAGGCCCAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGATGCG  >  NC_002947/571469‑571763
                                                                                                                                                    |                                                                                                                                                  
gcagGCCCAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAg                                                                                                                                                    <  1:58669/149‑1 (MQ=255)
      ccAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAAAAACCCAGGGCAACTGccc                                                                                                                                              >  4:70789/1‑149 (MQ=255)
               gCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGttttt                                                                                                                                     >  2:165717/1‑149 (MQ=255)
                    aGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGA‑CAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATgg                                                                                                                               <  2:170726/149‑1 (MQ=255)
                      gCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATggg                                                                                                                              >  2:190761/1‑149 (MQ=255)
                       ccccTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgc                                                                                                                             <  8:171092/149‑1 (MQ=255)
                         ccTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGTTGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgcag                                                                                                                           <  4:198174/149‑1 (MQ=255)
                         ccTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgcag                                                                                                                           >  3:366659/1‑149 (MQ=255)
                             agcgcccagcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGTCTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCAcc                                                                                                                       >  4:5907/1‑149 (MQ=255)
                             agcgcccagcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCTGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCAcc                                                                                                                       >  4:57306/1‑149 (MQ=255)
                             agcgcccagcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCAcc                                                                                                                       >  5:106743/1‑149 (MQ=255)
                             agcgcccagcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCAcc                                                                                                                       >  3:272428/1‑149 (MQ=255)
                               cgcccagcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACcgc                                                                                                                     <  2:114947/149‑1 (MQ=255)
                                 cccagcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATgg                                                                                                                               <  4:186879/137‑1 (MQ=255)
                                 cccagcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATgg                                                                                                                               >  3:186879/1‑137 (MQ=255)
                                  ccagcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGGTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTTTGGGCAGCACCGCGtt                                                                                                                  >  8:278639/1‑148 (MQ=255)
                                    agcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTAt                                                                                                                 >  6:95292/1‑148 (MQ=255)
                                    agcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTAt                                                                                                                 >  3:249130/1‑148 (MQ=255)
                                         cgcAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCATCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAg                                                                                                              <  5:195174/146‑1 (MQ=255)
                                         cgcAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAg                                                                                                              >  6:195174/1‑146 (MQ=255)
                                         cgcAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCa                                                                                                           >  7:240734/1‑149 (MQ=255)
                                              cAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCt                                                                                                      >  8:258967/1‑149 (MQ=255)
                                                gCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCttt                                                                                                    <  4:272428/149‑1 (MQ=255)
                                                gCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCttt                                                                                                    <  7:36353/149‑1 (MQ=255)
                                                 ccTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTc                                                                                                   <  8:22527/149‑1 (MQ=255)
                                                  cTGCGCCGCAGCCGTCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCa                                                                                                  <  6:371632/149‑1 (MQ=255)
                                                  cTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCa                                                                                                  <  5:159460/149‑1 (MQ=255)
                                                  cTGCGCCGCAGCCGGCGGCTGACTTCGACACCTTCGATGACGATATTCCGTTCTGATCAGAGCGTTAGGGCGTTCAGCAAGACCAGAAACCCAGGCCAGTTGCACTGGATTTTTTTATGTGAATCACCGCCTATCAGGCATCCCTTTc                                                                                                   >  8:238857/1‑148 (MQ=255)
                                                     gaagtcAGCCGCCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAg                                                                                                 >  6:374304/6‑147 (MQ=255)
                                                     cgccgcAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGt                                                                                                <  8:240734/148‑1 (MQ=255)
                                                     cgccgcAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTa                                                                                               <  7:308839/149‑1 (MQ=255)
                                                      gccgcAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGc                                                                                                       <  6:29621/140‑1 (MQ=255)
                                                      gccgcAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGc                                                                                                       >  5:29621/1‑140 (MQ=255)
                                                              cggcggCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGTAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgcag                                                                                                                           <  4:81503/112‑1 (MQ=255)
                                                              cggcggCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgcag                                                                                                                           >  3:81503/1‑112 (MQ=255)
                                                               ggcggcTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCgg                                                                                     <  1:165717/149‑1 (MQ=255)
                                                                     tGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTcc                                                                               >  1:213894/1‑149 (MQ=255)
                                                                                      aTGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCt                                                              >  1:342179/1‑149 (MQ=255)
                                                                                          cGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCa                                                                                                  <  3:360160/109‑1 (MQ=255)
                                                                                          cGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCa                                                                                                  >  4:360160/1‑109 (MQ=255)
                                                                                          cGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGcc                                                          <  5:180687/149‑1 (MQ=255)
                                                                                           gATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCa                                                         <  5:95292/149‑1 (MQ=255)
                                                                                            atatTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAg                                                        >  7:339587/1‑149 (MQ=255)
                                                                                             tatTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCa                                                                                                  >  2:148565/1‑106 (MQ=255)
                                                                                             tatTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCa                                                                                                  <  1:148565/106‑1 (MQ=255)
                                                                                               ttCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGtttt                                                                                                                                      >  6:3410/1‑68 (MQ=255)
                                                                                               ttCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGtttt                                                                                                                                      <  5:3410/68‑1 (MQ=255)
                                                                                                tCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTg                                                    >  7:334847/1‑149 (MQ=255)
                                                                                                 ccGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTg                                                    <  8:339587/148‑1 (MQ=255)
                                                                                                  cGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGt                                                  >  1:235585/1‑149 (MQ=255)
                                                                                                  cGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGt                                                  <  2:342179/149‑1 (MQ=255)
                                                                                                         aTCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGc                                            <  8:216528/148‑1 (MQ=255)
                                                                                                            agagCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCaaa                                         <  8:334847/148‑1 (MQ=255)
                                                                                                            agagCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAg                                        >  5:227472/1‑149 (MQ=255)
                                                                                                                cGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCa                                    <  6:106743/149‑1 (MQ=255)
                                                                                                                cGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCa                                    <  3:5907/149‑1 (MQ=255)
                                                                                                                     gggCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCAGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCt                               >  1:252578/1‑149 (MQ=255)
                                                                                                                     gggCGTTCAGCAAGACCAGAAACCCAGGCAAGTTGCGCTGGGTGTTTTTATGGGCAGCACCGCGGATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTACGGCGAATCAAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCt                               >  1:205750/1‑149 (MQ=255)
                                                                                                                           tCACCAAGACCTGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTggcgg                         <  3:57306/149‑1 (MQ=255)
                                                                                                                             aGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCGGTTCTTGGCGGCt                       <  2:235585/149‑1 (MQ=255)
                                                                                                                             aGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGtt                                 <  8:203898/139‑1 (MQ=255)
                                                                                                                             aGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGtt                                 >  7:203898/1‑139 (MQ=255)
                                                                                                                               cAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTgg                     >  2:131151/1‑149 (MQ=255)
                                                                                                                                 aGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGt                   <  1:190761/149‑1 (MQ=255)
                                                                                                                                  gACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTg                  >  3:316450/1‑149 (MQ=255)
                                                                                                                                   aCCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGAGTGc                 <  4:366659/149‑1 (MQ=255)
                                                                                                                                     cAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGt                   >  3:76346/1‑145 (MQ=255)
                                                                                                                                     cAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGt                   <  4:76346/145‑1 (MQ=255)
                                                                                                                                       gAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCAt              >  1:181572/1‑148 (MQ=255)
                                                                                                                                        aaaCCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCAt              >  7:83234/1‑147 (MQ=255)
                                                                                                                                          aCCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAgg          >  8:116880/1‑149 (MQ=255)
                                                                                                                                          aCCCAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAgg          >  6:228288/1‑149 (MQ=255)
                                                                                                                                           cccAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAggg         <  7:258967/149‑1 (MQ=255)
                                                                                                                                           cccAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAggg         <  6:227472/149‑1 (MQ=255)
                                                                                                                                           cccAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAgg                                              >  3:101597/1‑112 (MQ=255)
                                                                                                                                           cccAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAgg                                              <  4:101597/112‑1 (MQ=255)
                                                                                                                                           cccAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTgg                     >  5:133607/1‑137 (MQ=255)
                                                                                                                                           cccAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTgg                     <  6:133607/137‑1 (MQ=255)
                                                                                                                                              aGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGa      >  4:202908/1‑149 (MQ=255)
                                                                                                                                                   aGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTCGGCGGCTGGGTGCCATCAGGGCGATgcg  >  1:138756/1‑148 (MQ=255)
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GCAGGCCCAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGATGCG  >  NC_002947/571469‑571763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑