Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 660,596 T→C 8.6% Y548H (TAC→CAC)  speA → biosynthetic arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947660,5960TC 8.6% 193.8 / 7.3 70Y548H (TAC→CAC) speAbiosynthetic arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (29/35);  new base C (2/4);  total (31/39)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

GGCGTGCGGTATTGCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACATGCTGCGCTACGTGCACCTGTCGC  >  NC_002947/660450‑660743
                                                                                                                                                  |                                                                                                                                                   
ggcgTGCGGTATTGCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTAc                                                                                                                                                   <  8:145655/149‑1 (MQ=255)
  cgTGCGGTATTGCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCa                                                                                                                                                 <  1:64956/149‑1 (MQ=255)
    tGCGGTATTGCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTAGGCACCCACCAgg                                                                                                                                               <  3:322918/149‑1 (MQ=255)
      cGGTATTGCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTAGGCACCCACCAGGaa                                                                                                                                             <  8:38880/149‑1 (MQ=255)
      cGGTATTGCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTAGGCACCCACCAGGaa                                                                                                                                             <  4:140795/149‑1 (MQ=255)
       ggTATTGCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTAGGCACCCACCAGGaac                                                                                                                                            <  4:16816/149‑2 (MQ=255)
             gccggACCAGACGCGCGATTCCGCCCGCCACATCAACCACCACCTTGACGACCAGAGCCTCGAAACCAGCCTGCCGGGGCATGCGGTGAAGGAGGGGGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTgg                                                                                                                                      <  8:258088/146‑1 (MQ=255)
             gCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTg                                                                                                                                       <  5:133531/148‑1 (MQ=255)
              cAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTggg                                                                                                                                     >  8:99506/1‑149 (MQ=255)
              cAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTggg                                                                                                                                     >  5:358476/1‑149 (MQ=255)
              cAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTgg                                                                                                                                      <  4:365715/148‑1 (MQ=255)
              cAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTgg                                                                                                                                      <  3:226855/148‑1 (MQ=255)
                   ccTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACa                                                                                                                                >  7:144176/1‑149 (MQ=255)
                   ccTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACa                                                                                                                                <  3:277298/149‑1 (MQ=255)
                      gACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGt                                                                                                                                    >  5:343068/1‑142 (MQ=255)
                      gACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGt                                                                                                                                    <  6:343068/142‑1 (MQ=255)
                       aCTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACacg                                                                                                                              <  2:285387/147‑3 (MQ=255)
                       aCTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATg                                                                                                                              <  6:8887/147‑1 (MQ=255)
                         ttGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGcac                                                                                                                           >  5:145134/1‑148 (MQ=255)
                            cGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAATTCCTGGGt                                                                                                                                    >  1:151969/1‑136 (MQ=255)
                            cGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGt                                                                                                                                    <  2:151969/136‑1 (MQ=255)
                                 ccGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGtt                                                                                                                   <  4:170892/148‑1 (MQ=255)
                                  cGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCg                                                                                                                 >  5:77235/1‑149 (MQ=255)
                                  cGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCg                                                                                                                 >  3:92466/1‑149 (MQ=255)
                                    aCGGCAAGATCTACCAGTACGTTGAAGAGCAGAGCATCGAAACCATGATGGCAGTGCATGGGGTGAAGGATGGTGATGATTACCTGCTTGGCGTGTTCCTGGTGGGTGCCTACGAGGAAATCCTGGGTGACATGCACAACCTGTTCGGt                                                                                                               <  1:358961/149‑1 (MQ=255)
                                        cAAGATCAACCAGTACGTTGACGAGAAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGaca                                                                                                           <  6:358476/149‑1 (MQ=255)
                                         aaGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGATGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGacac                                                                                                          >  2:352367/1‑149 (MQ=255)
                                           gATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACAcc                                                                                                         >  4:51687/1‑148 (MQ=255)
                                             tCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGAt                                                                                                      >  3:87206/1‑149 (MQ=255)
                                                   aGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCATGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTg                                                                                                <  8:205052/149‑1 (MQ=255)
                                                    gTACGTTGAGGAGCATAGCTTCGAAACCTGCATGCCGGTGCATGCTGTGAAGGAGGGTGTGGATTAGCTGCTGGGCGTGTTGCTGGTGGGTGCCTACCATTTAATCCTTTGTGAAATGCTCATGCTGTTGGGTGACACCGATTCGGTGa                                                                                               <  6:77235/149‑1 (MQ=255)
                                                    gTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGa                                                                                               <  3:215693/149‑1 (MQ=255)
                                                     tACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGATGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGaa                                                                                              <  7:157075/149‑1 (MQ=255)
                                                       cGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAAc                                                                                             <  2:156779/148‑1 (MQ=255)
                                                        gTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACa                                                                                            >  5:57786/1‑148 (MQ=255)
                                                        gTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACAt                                                                                           <  3:51687/149‑1 (MQ=255)
                                                            aCGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTAc                                                                                       >  2:361947/1‑149 (MQ=255)
                                                                gcagagcaTCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAg                                                                                    >  4:232651/1‑148 (MQ=255)
                                                                gcagagcaTCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAg                                                                                    >  4:133587/1‑148 (MQ=255)
                                                                    agcaTCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGc                                                                               >  4:132030/1‑149 (MQ=255)
                                                                      caTCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGt                                                                                                 <  4:162301/129‑1 (MQ=255)
                                                                      caTCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGt                                                                                                 >  3:162301/1‑129 (MQ=255)
                                                                        tCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCg                                                                                                   >  2:359403/1‑125 (MQ=255)
                                                                        tCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCg                                                                                                   <  1:359403/125‑1 (MQ=255)
                                                                        tCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGAc                                                                           <  3:132030/149‑1 (MQ=255)
                                                                        tCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGAc                                                                           <  6:57786/149‑1 (MQ=255)
                                                                           aaaCCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCGTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGc                                                                        >  4:222394/1‑149 (MQ=255)
                                                                            aaCCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCa                                                                       <  6:145134/149‑1 (MQ=255)
                                                                              ccAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGc                                                                     <  8:144176/149‑1 (MQ=255)
                                                                                  cATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCgtgt                                                                 <  7:99506/149‑1 (MQ=255)
                                                                                                            gTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATc                                                   <  4:249716/137‑1 (MQ=255)
                                                                                                            gTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATc                                                   >  3:249716/1‑137 (MQ=255)
                                                                                                               aGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGacac                                     >  2:255480/1‑148 (MQ=255)
                                                                                                                 gATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGc                                                                               <  6:115304/104‑1 (MQ=255)
                                                                                                                 gATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGc                                                                               >  5:115304/1‑104 (MQ=255)
                                                                                                                       ctgctgGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGa                            <  4:92466/149‑1 (MQ=255)
                                                                                                                         gctgGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACa                          >  7:189022/1‑149 (MQ=255)
                                                                                                                         gctgGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACa                          >  2:305662/1‑149 (MQ=255)
                                                                                                                          ctgGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGAc                           <  1:361947/147‑1 (MQ=255)
                                                                                                                            gggCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTAGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACAtgc                       >  3:191920/1‑149 (MQ=255)
                                                                                                                            ccaggatttCCTGGTAGGCACCCACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACAtgc                       >  8:303893/8‑149 (MQ=255)
                                                                                                                             ggCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACAtgct                      <  3:367469/149‑1 (MQ=255)
                                                                                                                             caggatttCCTGGTAGGCACCCACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACAtgct                      >  5:378064/7‑149 (MQ=255)
                                                                                                                                   ttCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACATGCTGCGCTa                >  8:322929/1‑149 (MQ=255)
                                                                                                                                      cTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACATGCTGCGCTACg              <  1:255480/148‑1 (MQ=255)
                                                                                                                                       tggtggGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGaa                              >  6:219751/1‑131 (MQ=255)
                                                                                                                                       tggtggGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGaa                              <  5:219751/131‑1 (MQ=255)
                                                                                                                                         gtgggtgCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACATGCTGCGCTACGTGCa          <  4:87206/149‑1 (MQ=255)
                                                                                                                                             gtgCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACATGCTGCGCTACGTGCACCTg      >  3:297427/1‑149 (MQ=255)
                                                                                                                                                 cTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACATGCTGCGCTACGTGCACCTGTCGc  >  4:191967/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGCGTGCGGTATTGCAGGACCTGACTTGCGATTCCGACGGCAAGATCAACCAGTACGTTGACGAGCAGAGCATCGAAACCAGCATGCCGGTGCATGCGGTGAAGGAGGGTGAGGATTACCTGCTGGGCGTGTTCCTGGTGGGTGCCTACCAGGAAATCCTGGGTGACATGCACAACCTGTTCGGTGACACCGATTCGGTGAACATCTACCAGAATGCCGACGGCAGCGTGTACCACGCCGGTATCGAAACCCACGACACCATCGAAGACATGCTGCGCTACGTGCACCTGTCGC  >  NC_002947/660450‑660743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑