Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 682,199 G→C 9.6% F358L (TTC→TTG)  PP_0584 ← methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947682,1990GC 9.6% 178.8 / 6.1 61F358L (TTC→TTG) PP_0584methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base G (27/28);  new base C (2/4);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCG  >  NC_002947/682055‑682338
                                                                                                                                                |                                                                                                                                           
cATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAAc                                                                                                                                          >  1:314997/1‑148 (MQ=255)
  tGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAg                                                                                                                                       <  4:95668/149‑1 (MQ=255)
     ccACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTg                                                                                                                                    >  8:31350/1‑149 (MQ=255)
        cGGCTTCGCGCTGGAGGCCGGCCGCCTCGTCCATGTCACTGGAGACGCGGGTCGAGCTGGTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGATTGGCACCGAGGAACTGGTTCAACCAGttcctc                                                                                                                                 <  7:347673/149‑5 (MQ=255)
         ggCTTCGCGCTGGCGGCCGGCCGCCGCGGCCATGGCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTCAACCAGttcctcg                                                                                                                                <  8:168385/149‑6 (MQ=255)
           cTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAg                                                                                                                                       <  8:81094/140‑1 (MQ=255)
           cTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAg                                                                                                                                       >  7:81094/1‑140 (MQ=255)
                 gcTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGcgcg                                                                                                                        >  7:184506/1‑149 (MQ=255)
                     gcggcCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGttt                                                                                                                    <  2:314997/149‑1 (MQ=255)
                       ggccggccGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTtcg                                                                                                                  >  3:107841/1‑149 (MQ=255)
                       ggccggccGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTtcg                                                                                                                  <  8:184506/149‑1 (MQ=255)
                        gccggccgCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTtcgt                                                                                                                 >  2:123522/1‑149 (MQ=255)
                         ccggccgCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGACGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCTCGGTTtcgtc                                                                                                                <  8:166623/149‑1 (MQ=255)
                                            cACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAgg                                                                                             <  7:31350/149‑1 (MQ=255)
                                            cACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAgg                                                                                             >  4:60075/1‑149 (MQ=255)
                                             aCTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGt                                                                                            >  2:1087/1‑149 (MQ=255)
                                                      gcgGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGggt                                                                                     >  1:112824/1‑147 (MQ=255)
                                                      gccggacGAGCTGCTGGACGTGCTGAGGATATTGCTGGCACCAGAGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGCAACTGGATGAACCAGATGGCCAGTTGCGCGGGTTCGTCGCGGCCGCGGATATCCAGGTTCCGggt                                                                                     >  2:272257/7‑147 (MQ=255)
                                                        gggTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGggtcagg                                                                                 <  1:1087/149‑1 (MQ=255)
                                                                 tgctgGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGc                                                                                         >  3:178587/1‑132 (MQ=255)
                                                                 tgctgGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGc                                                                                         >  1:129448/1‑132 (MQ=255)
                                                                 tgctgGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGc                                                                                         >  1:134364/1‑132 (MQ=255)
                                                                 tgctgGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGc                                                                                         <  2:129448/132‑1 (MQ=255)
                                                                 tgctgGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGc                                                                                         <  2:134364/132‑1 (MQ=255)
                                                                 tgctgGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGc                                                                                         <  4:178587/132‑1 (MQ=255)
                                                                  gctgGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGc                                                                                         >  2:59656/1‑131 (MQ=255)
                                                                     ggACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCtt                                                                    >  7:224606/1‑149 (MQ=255)
                                                                      gACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTg                                                                   <  2:112824/149‑1 (MQ=255)
                                                                         gTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGc                                                                <  4:104160/149‑1 (MQ=255)
                                                                            cTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAAt                                                             <  3:60075/149‑1 (MQ=255)
                                                                            cTGAGGATCTTGCTGACAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAAt                                                             <  4:124363/149‑1 (MQ=255)
                                                                              gAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATg                                                            >  6:32356/1‑148 (MQ=255)
                                                                               aGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGtt                                                                                                                     <  6:74414/90‑1 (MQ=255)
                                                                               aGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTc                                                          <  3:285050/149‑1 (MQ=255)
                                                                                    cTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTc                                                       >  7:348587/1‑147 (MQ=255)
                                                                                         tGGCAGCAGAGCCGATGTGGTGGATCAGGGTGGGAATGGCACCGAGGAACTGGTTGAACCAGATGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTACAGGATGCGGGTCAGGTCACCGTCACCTTGGGCAATGTCTTCCAGGcga                                                >  6:308741/1‑147 (MQ=255)
                                                                                                     cGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCcgc                                                                                                        >  3:199883/1‑81 (MQ=255)
                                                                                                     cGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCcgc                                                                                                        <  4:199883/81‑1 (MQ=255)
                                                                                                      gATGTGCTGGATCAGGCTGCGAATGGCTCCGTGGAACTGGTTGAACGAGTTGGCCAGTTGCGCGTTTTCGTCGCGGCCGCGGATTTCCTGGTTGCGGGTTAGGTCACCCTCTCCTTGGGCAATGTCTTCCAGGACATTGGTGACGCTGt                                   <  1:272257/149‑1 (MQ=255)
                                                                                                      gATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGt                                   <  4:107841/149‑1 (MQ=255)
                                                                                                      gATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGt                                   <  4:29402/149‑1 (MQ=255)
                                                                                                           gCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATc                              <  5:32356/149‑1 (MQ=255)
                                                                                                               gATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCt                                                                          >  3:208495/1‑101 (MQ=255)
                                                                                                               gATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCt                                                                          <  4:208495/101‑1 (MQ=255)
                                                                                                                   aGGCGGCGAAGGGCACCGTGAAACTGGTTCAACCAGTTATCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCa                       <  1:123522/148‑1 (MQ=255)
                                                                                                                     gCTGCGAATGGCACCGAGGAACTGGTTGAACGAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGa                    <  1:68239/149‑1 (MQ=255)
                                                                                                                     gCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGc                                      <  3:73232/131‑1 (MQ=255)
                                                                                                                     gCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGc                                      >  4:73232/1‑131 (MQ=255)
                                                                                                                     gCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGa                    >  5:94514/1‑149 (MQ=255)
                                                                                                                           aaTGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGTGCACCCGATCaaca              <  8:224606/149‑4 (MQ=255)
                                                                                                                                 aCCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCg         >  2:213497/1‑148 (MQ=255)
                                                                                                                                 aCCGAGGAACTGGTTGAACCAGGTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCg         >  7:233565/1‑148 (MQ=255)
                                                                                                                                  ccGAGGAACTTGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGaa       <  7:18324/149‑1 (MQ=255)
                                                                                                                                   cGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAg      >  7:88523/1‑149 (MQ=255)
                                                                                                                                     aGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGc    <  8:348587/149‑1 (MQ=255)
                                                                                                                                      ccaaCTGGTTCAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGcc   >  8:124076/3‑149 (MQ=255)
                                                                                                                                       gAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGCCGCTGTTCATCGGGCGCACGATCAGTTTGGCGCAGGCCg  >  8:172258/1‑149 (MQ=255)
                                                                                                                                       gAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAgg     <  6:202350/146‑1 (MQ=255)
                                                                                                                                       gAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAgg     >  5:202350/1‑146 (MQ=255)
                                                                                                                                             gTTCAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGgc                          <  7:135201/119‑1 (MQ=255)
                                                                                                                                             gTTCAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGgc                          >  8:135201/1‑119 (MQ=255)
                                                                                                                                                |                                                                                                                                           
CATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTTGGCCAGTTGCGCGGTTTCGTCGCGGCCGCGGATTTCCAGGTTGCGGGTCAGGTCACCCTCACCTTGGGCAATGTCTTCCAGGCCATTGGTGACGCTGTTGATCGGGCGCACGATCAGTTTGGCGAAGGCCG  >  NC_002947/682055‑682338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑