BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	160,513	A→C	5.1%	F65V (TTC→GTC)	PP_0150 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	160,513	0	A	C	5.1%	306.2 / 4.5	95	F65V (TTC→GTC)	PP_0150	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (43/47); new base C (3/2); total (46/49)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

CCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGC  >  NC_002947/160368‑160660

ccAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCt                                                                                                                                                   >  4:318585/1‑149 (MQ=255)

 agatctcgcacgaGGTTCTCAGTGCGAGTATGGCTTCAGAGAATACCTGGTCGCTGGCGGCGGGCGCTTCCGGTACTCGGTCGCGCTGGGTTTTGAAGGCCGCCTCGTGTTGTACGCGGCGGATGTCACCGTTGCTGGCGACGAAGCtt                                                                                                                                                  <  4:148294/143‑1 (MQ=255)

  aGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTg                                                                                                                                                 >  2:70565/1‑149 (MQ=255)

     cacgcacgCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAg                                                                                                                                              <  5:241337/149‑1 (MQ=255)

      acgcacgcGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGc                                                                                                                                             >  3:358120/1‑149 (MQ=255)

      acgcacgcGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGc                                                                                                                                             >  5:162011/1‑149 (MQ=255)

        gcacgcGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCtt                                                                                                                                                  >  3:148294/1‑142 (MQ=255)

        gcacgcGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTAGTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGcgt                                                                                                                                           >  4:234970/1‑149 (MQ=255)

              gcTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGACGCTGCAAGcttcgtcgc                                                                                                                                     <  3:342/149‑2 (MQ=255)

              gcTTCACAGTGCGAGGATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGcgtcgtcgt                                                                                                                                     <  7:58490/149‑1 (MQ=255)

               cTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTg                                                                                                                                    <  1:186051/149‑1 (MQ=255)

                ttCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTgg                                                                                                                                   >  4:84047/1‑149 (MQ=255)

                ttCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTgg                                                                                                                                   >  4:116552/1‑149 (MQ=255)

                ttCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTgg                                                                                                                                   >  6:237234/1‑149 (MQ=255)

                   acaGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGACGCTGCAAGcttcgtcgc                                                                                                                                     <  7:268111/144‑2 (MQ=255)

                   acaGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGACGCTGCAAGcttcgtcgc                                                                                                                                     >  8:268111/1‑143 (MQ=255)

                   acaGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGAAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGATGGCGACGAAGCTTGCAGCGTCGTCGTGGGCt                                                                                                                                >  3:248018/1‑149 (MQ=255)

                       tGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTcgc                                                                                                                            >  2:104796/1‑149 (MQ=255)

                          gAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGca                                                                                                                         <  6:322565/149‑1 (MQ=255)

                          gAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGca                                                                                                                         <  3:116552/149‑1 (MQ=255)

                                ggCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTAGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTAGCAGCGTCGTCGTGGGCTTCGCGCACCACtt                                                                                                                   >  5:197208/1‑149 (MQ=255)

                                 gCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACtt                                                                                                                   <  4:319038/148‑1 (MQ=255)

                                  cTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTg                                                                                                                 <  3:234970/149‑1 (MQ=255)

                                  cTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTg                                                                                                                 <  6:52450/149‑1 (MQ=255)

                                  cTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTg                                                                                                                 <  3:169354/149‑1 (MQ=255)

                                  cTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTg                                                                                                                 <  1:104796/149‑1 (MQ=255)

                                  cTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTg                                                                                                                 >  7:39573/1‑149 (MQ=255)

                                  cTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTg                                                                                                                 >  8:221580/1‑149 (MQ=255)

                                   ttCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTg                                                                                                                 >  8:127482/1‑148 (MQ=255)

                                     cAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGa                                                                                                               <  1:279629/148‑1 (MQ=255)

                                        ccAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcg                                                                                                           >  8:241965/1‑149 (MQ=255)

                                         cAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGcgtcgtcgt                                                                                                                                     <  2:305615/122‑1 (MQ=255)

                                         cAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGcgtcgtcgt                                                                                                                                     >  1:305615/1‑122 (MQ=255)

                                         cAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcgc                                                                                                          <  6:162011/149‑1 (MQ=255)

                                               cTGGTCGCTGGCGCCGCGCGCTTCCGGTTCTCGCTCGCGCAGGGTATTGAAGGACGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATc                                                                                                     <  7:241965/148‑1 (MQ=255)

                                                   tCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAggt                                                                                                >  6:40087/1‑149 (MQ=255)

                                                      cTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAggtggt                                                                                             <  3:84047/149‑1 (MQ=255)

                                                       tGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAggtggtg                                                                                            <  3:318585/149‑1 (MQ=255)

                                                       tGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAggtggtg                                                                                            >  1:181976/1‑149 (MQ=255)

                                                               cgcgcTTCCGGTACTCGCTGGCGCAGGGTTTTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGTGGAGGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTTGTG‑GTGTCTGa                                                                                   <  6:197208/149‑1 (MQ=255)

                                                                gcgcTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAg                                                                                  <  5:237234/149‑1 (MQ=255)

                                                                 cgcTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAggtggtg                                                                                            <  4:246674/139‑1 (MQ=255)

                                                                 cgcTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAggtggtg                                                                                            >  3:246674/1‑139 (MQ=255)

                                                                 cgcTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAgg                                                                                 >  3:368091/1‑149 (MQ=255)

                                                                    ttCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTg                                                                               >  4:145924/1‑148 (MQ=255)

                                                                      ccGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGa                                                                              <  6:244798/147‑1 (MQ=255)

                                                                      ccGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGa                                                                              >  5:244798/1‑147 (MQ=255)

                                                                      caggTACTCGCTCGCGCTGGGTAGTGAAGGCCGCCTCGATTTGTACGCCGCGGATGTCACAGTTGCTGGAGACGAATCTTGCATCTTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCTCGGATCGAGGTTGTG‑GTGTCTGAgtttaa                                                                             <  4:248018/146‑6 (MQ=255)

                                                                       cGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCAGCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGt                                                                           <  4:358120/149‑1 (MQ=255)

                                                                       cGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGt                                                                           >  7:44/1‑149 (MQ=255)

                                                                       cGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGt                                                                           >  2:194122/1‑149 (MQ=255)

                                                                         gTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGtc                                                                          >  7:242863/1‑148 (MQ=255)

                                                                            ctcgctcgCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGtcgat                                                                       <  1:194122/148‑1 (MQ=255)

                                                                               gctcgcGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcgc                                                                                                          <  1:150701/111‑1 (MQ=255)

                                                                               gctcgcGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcgc                                                                                                          >  2:150701/1‑111 (MQ=255)

                                                                               gctcgcGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGtcgatcg                                                                     <  4:368091/147‑1 (MQ=255)

                                                                                   gcgcAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGc                                                               >  2:281246/1‑149 (MQ=255)

                                                                                     gcAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑gtgt                                                                                       >  7:296477/1‑123 (MQ=255)

                                                                                     gcAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑gtgt                                                                                       <  8:296477/123‑1 (MQ=255)

                                                                                        gggTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAg                                                          <  8:114615/149‑1 (MQ=255)

                                                                                        gggTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAg                                                          <  5:40087/149‑1 (MQ=255)

                                                                                              tGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGgcacgc                                                    >  8:117283/1‑149 (MQ=255)

                                                                                                aaGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGgcacgcac                                                  >  2:87473/1‑149 (MQ=255)

                                                                                                aaGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GGGTCTGAGGTGAAGTCGATCGAACGGCCAAAGgcacgcac                                                  >  1:124797/1‑149 (MQ=255)

                                                                                                aaGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTC‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGgcacgcac                                                  >  5:317731/1‑149 (MQ=255)

                                                                                                     cgccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACgatga                                             <  5:142747/149‑1 (MQ=255)

                                                                                                      gccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACgatgat                                            <  1:87473/149‑1 (MQ=255)

                                                                                                       ccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGatgatg                                           <  6:317731/149‑1 (MQ=255)

                                                                                                        cTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGt                                          >  1:44612/1‑149 (MQ=255)

                                                                                                          cGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGt                                                                           <  8:205942/114‑1 (MQ=255)

                                                                                                           gAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGaa                                                                             <  4:218416/111‑1 (MQ=255)

                                                                                                           gAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGaa                                                                             >  3:218416/1‑111 (MQ=255)

                                                                                                           gAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGtt                                         <  3:152506/147‑1 (MQ=255)

                                                                                                           gAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGc                                       <  8:39573/149‑1 (MQ=255)

                                                                                                             gTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCtg                                     <  7:221580/149‑1 (MQ=255)

                                                                                                              ttGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGCGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCtg                                     <  1:281246/149‑1 (MQ=255)

                                                                                                              ttGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCtg                                     <  2:181976/148‑1 (MQ=255)

                                                                                                                gTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCtggt                                   <  7:127482/148‑1 (MQ=255)

                                                                                                                 tACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCtggtg                                  <  5:228457/148‑1 (MQ=255)

                                                                                                                   cgccgcGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGc                                >  7:259589/1‑148 (MQ=255)

                                                                                                                     ccgcGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGccac                             >  1:254082/1‑149 (MQ=255)

                                                                                                                     ccgcGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGccac                             >  3:32078/1‑149 (MQ=255)

                                                                                                                                aCCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGTGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCtgtg                  >  1:202521/1‑149 (MQ=255)

                                                                                                                                 ccGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGc                 <  4:32078/149‑1 (MQ=255)

                                                                                                                                  cGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAggtggtg‑gt                                                                                         >  8:58951/1‑76 (MQ=255)

                                                                                                                                  cGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAggtggtg‑gt                                                                                         <  7:58951/76‑1 (MQ=255)

                                                                                                                                      gCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTCGCTGGTGGCCACCAGGGTCTGTGCCTgg             <  3:145924/148‑1 (MQ=255)

                                                                                                                                        ccacgacgaCGCTGCAAGCTTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGc           >  4:121276/4‑148 (MQ=255)

                                                                                                                                          gCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAg        <  8:242863/149‑1 (MQ=255)

                                                                                                                                          acgacgaCGCTGCAAGCTTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAg        >  8:289444/2‑149 (MQ=255)

                                                                                                                                            gacgaAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCtggtg                                  >  3:188777/1‑121 (MQ=255)

                                                                                                                                            gacgaAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCtggtg                                  <  4:188777/121‑1 (MQ=255)

                                                                                                                                            gacgaAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGt      >  2:195807/1‑149 (MQ=255)

                                                                                                                                            gacgaAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGGCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGt      >  2:159414/1‑149 (MQ=255)

                                                                                                                                             acgaAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTc     <  8:44/149‑1 (MQ=255)

                                                                                                                                                 aGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGc  >  7:263459/1‑148 (MQ=255)

CCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTG‑GTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGC  >  NC_002947/160368‑160660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`