| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 714,186 | T→A | 7.2% | V67D (GTC→GAC) | ispH → | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 714,186 | 0 | T | A | 7.2% | 202.8 / 5.1 | 72 | V67D (GTC→GAC) | ispH | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base T (32/33); new base A (3/2); total (36/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
GCGATCGAGATCGTCAACCGCGCGCTGGAAGTCTTCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAGTGGCCAAGTACAGCCGCGATGGCCGTGAGTG > NC_002947/714038‑714333 | gCGATCGAGATCGTCAACCGCGCGCTGGAAGTCTTCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATgt > 5:157904/1‑149 (MQ=255) agaTCGTAAACCGCGCGCTGGAAGTCTTCGGACCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCAGCTTTGTCGAAGAGCTGGACCATGTGCCGGACGATgtcatcgt > 4:201460/1‑148 (MQ=255) gTCAACCGCGCGCTGGAAGTCTTCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCtt > 8:205695/1‑149 (MQ=255) aaCCGCGCGCTGGAAGTCTTCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCa < 1:59804/148‑1 (MQ=255) cgcgcgCTGGAAGTCTTCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGc > 8:212953/1‑149 (MQ=255) gcgcgcTGGAAGTCTTCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGcc > 4:148164/1‑149 (MQ=255) cgcgcTGGAAGTCTTCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGcc > 4:258978/1‑148 (MQ=255) gcgcTGGAAGTCTTCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCa < 4:48514/149‑1 (MQ=255) cTGGAAGTCTTCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCtt < 8:307361/137‑1 (MQ=255) cTGGAAGTCTTCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCtt > 7:307361/1‑137 (MQ=255) tGGAAGTCTTCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGCCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCAACTTCAGTGCCAACgga > 6:260250/1‑148 (MQ=255) cTTCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTcc < 7:212953/148‑1 (MQ=255) ttCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCa < 3:55342/130‑1 (MQ=255) ttCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCa > 4:55342/1‑130 (MQ=255) cGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAgg > 3:139934/1‑149 (MQ=255) ccgATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGACATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCg > 7:172538/1‑149 (MQ=255) gATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGTAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCgg > 1:118574/1‑148 (MQ=255) gcCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAgctgct > 8:98973/1‑149 (MQ=255) ccACGAAGTGGTGCACAACAAGTTTGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGctgctg > 2:84056/1‑149 (MQ=255) ccACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGctgctg < 2:118574/149‑1 (MQ=255) cACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACGGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAgct > 1:259373/1‑144 (MQ=255) cACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAgct < 2:259373/144‑1 (MQ=255) cACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTgg < 6:189964/149‑1 (MQ=255) aaGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGt < 4:218644/149‑1 (MQ=255) gtggtgCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTgg < 7:205695/149‑1 (MQ=255) tggtgCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGACATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGc < 8:172538/149‑1 (MQ=255) gtgCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTCTTTCCCAGGCCGTGCCGCAGGAAGCTGCTGGCCGTGGCCt > 3:62815/1‑149 (MQ=255) gCACAACACGTACGTGG‑GGCACCCCTGCGGTACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGaa < 5:287917/149‑1 (MQ=255) gCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGaa > 3:288754/1‑130 (MQ=255) gCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGaa < 4:288754/130‑1 (MQ=255) gCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGAGCACACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTg > 5:91171/1‑148 (MQ=255) acaacaAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGa > 2:57396/1‑147 (MQ=255) aacaaGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGgtgt > 8:21293/1‑104 (MQ=255) aacaaGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGgtgt < 7:21293/104‑1 (MQ=255) aacaaGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGc < 6:154231/141‑1 (MQ=255) aacaaGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGc > 5:154231/1‑141 (MQ=255) aacaaGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGCCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAgt > 2:117655/1‑149 (MQ=255) aacaaGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGATGGACAAAGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAgt > 5:257765/1‑149 (MQ=255) gTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAgtgt > 6:171005/1‑146 (MQ=255) gTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAgtgt < 5:171005/146‑1 (MQ=255) gTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTc > 3:17740/1‑148 (MQ=255) tCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTTGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGAt < 5:28947/149‑1 (MQ=255) gAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCa > 3:332777/1‑145 (MQ=255) gAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCa < 4:332777/145‑1 (MQ=255) gAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTg > 2:28108/1‑149 (MQ=255) ttGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCt > 1:152522/1‑149 (MQ=255) gCGTAACCGCGGCGCCATCTTTGTCGTAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTg < 1:146752/148‑1 (MQ=255) gCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCt < 7:98973/147‑1 (MQ=255) gTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGt > 6:258317/1‑148 (MQ=255) gTAACCCCGGCGCAATC‑TTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGa < 4:228467/149‑1 (MQ=255) tAACCTCGTCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCCCGGTGTTTCCCAGGCCGTTCGGCTGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGa < 3:201460/149‑1 (MQ=255) tAACCGCGGCGCCATCTTTGTCGAAGAGCTGGCCCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCCGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGa > 2:193791/1‑149 (MQ=255) tAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGa < 6:157904/149‑1 (MQ=255) ccGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGAcc > 7:53583/1‑148 (MQ=255) gcggcgCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAg < 1:57396/149‑1 (MQ=255) ccggcggCATCTTTGTCGAAGAGCTGGACCATGTGGCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGGCGTGGGGCAGGTAGCTGGTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAg < 4:62815/148‑1 (MQ=255) cATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGCCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTcaca < 1:117655/149‑1 (MQ=255) aTCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACAt > 3:117554/1‑149 (MQ=255) tttCGAAGAGCTGGAACATGTTCCGTACTATGTCATCGTCATCTTCAGTGCCCACTGTGTTTCCCATTCCGTGCGGCAGGAATCTTGTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGGCCGCTGGTGACCAAGGTTCACATctat < 8:225996/146‑4 (MQ=255) tGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATc < 3:258978/145‑1 (MQ=255) tGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCg < 5:258317/146‑1 (MQ=255) tGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGaa < 3:148164/148‑1 (MQ=255) gTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAg < 5:260250/148‑1 (MQ=255) tCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAGt < 4:17740/148‑1 (MQ=255) agCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAGTGGCCAAg < 4:139934/149‑1 (MQ=255) ggACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAGTGGCCAAGTa > 3:334312/1‑147 (MQ=255) gACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAGTGGCCAAGTACAg > 3:339445/1‑149 (MQ=255) ccAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAGTGGCCAAGTACAGcc > 4:312019/1‑149 (MQ=255) gTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAGTGGCCAAGTACAGCCGCGa < 4:117554/149‑1 (MQ=255) gatgACGATGACATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGaa > 8:16808/4‑126 (MQ=255) gatgACGATGACATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGaa < 7:16808/123‑1 (MQ=255) gatgACGATGACATCGTCATCTTCAGTGCCCACGGAGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAGTGGCCAAGTACAGCCGCGATgg > 1:55455/4‑149 (MQ=255) ggATGATGTCATAGTCATGTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAGTGGCCAAGTACAGCCGCGATGGcc < 6:257765/149‑1 (MQ=255) gACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAGTGGCCAAGTACAGCCGCGATGGCCg > 7:175998/1‑149 (MQ=255) gtcatcgtcatcTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAGTGGCCAAGTACAGCCGCGATGGCCGTGAGt < 4:334312/148‑1 (MQ=255) tcatcgtcatcTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAGTGGCCAAGTACAGCCGCGATGGCCGTGAGTg > 7:149588/1‑148 (MQ=255) | GCGATCGAGATCGTCAACCGCGCGCTGGAAGTCTTCGGCCCGCCGATCTATGTGCGCCACGAAGTGGTGCACAACAAGTTCGTGGTGGAAGACTTGCGTAACCGCGGCGCCATCTTTGTCGAAGAGCTGGACCAGGTGCCGGACGATGTCATCGTCATCTTCAGTGCCCACGGTGTTTCCCAGGCCGTGCGGCAGGAAGCTGCTGGCCGTGGCCTGAAAGTGTTCGATGCCACTTGCCCGCTGGTGACCAAGGTTCACATCGAAGTGGCCAAGTACAGCCGCGATGGCCGTGAGTG > NC_002947/714038‑714333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |