Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 764,568 G→A 6.0% G259G (GGC→GGT)  PP_0657 ← amino acid ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947764,5680GA 6.0% 204.6 / 2.2 69G259G (GGC→GGT) PP_0657amino acid ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (35/28);  new base A (2/2);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

AGCGCCAGCCAGGTCAGCAGTAACCGGGTGCGAAAGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCG  >  NC_002947/764423‑764715
                                                                                                                                                 |                                                                                                                                                   
aGCGCCAGCCAGGTCAGCAGTAACCGGGTGCGAAAGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCa                                                                                                                                                  >  7:62820/1‑149 (MQ=255)
   gccagccaGGTCAGCAGTAACCGGGTGCGAAAGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCa                                                                                                                                               <  8:62820/149‑1 (MQ=255)
     cagccagGTCAGCAGTAACCGGGTGCGAAAGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGc                                                                                                                                             >  2:120872/1‑149 (MQ=255)
          agGTCAGCAGTAACCGGGTGCGAAAGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCa                                                                                                                                        >  4:340098/1‑149 (MQ=255)
                gcagTAACCGGGTGCGAAAGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGTCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCgg                                                                                                                                  <  2:234693/149‑1 (MQ=255)
                 cagTAACCGGGTGCGAAAGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTACCATCAGCCTTCAGCTCGGc                                                                                                                                 >  6:132704/1‑149 (MQ=255)
                    tAACCGGGTGCGAAAGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCTCGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGaa                                                                                                                              >  7:63516/1‑149 (MQ=255)
                    tAACCGGGTGCGAAAGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGaa                                                                                                                              <  1:120872/149‑1 (MQ=255)
                      aCCGGGTGCGAAAGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAaca                                                                                                                            >  2:80448/1‑149 (MQ=255)
                        cGGGTGCGAAAGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACAcc                                                                                                                          >  7:76953/1‑149 (MQ=255)
                           gTGCGAAAGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTcg                                                                                                                       >  2:89882/1‑149 (MQ=255)
                                 aaGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCa                                                                                                                                               <  7:247167/119‑1 (MQ=255)
                                 aaGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCa                                                                                                                                               >  8:247167/1‑119 (MQ=255)
                                    ccGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTg                                                                                                              <  2:246311/149‑1 (MQ=255)
                                    ccGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTg                                                                                                              <  2:69655/149‑1 (MQ=255)
                                     cGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGCTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTgg                                                                                                             >  2:49170/1‑149 (MQ=255)
                                      gAACAGGCCGGCGCCCGCAGGCTTTGCCTCGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGc                                                                                                            <  8:63516/149‑1 (MQ=255)
                                        aCAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCAt                                                                                                          <  2:287345/149‑1 (MQ=255)
                                         cAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTACCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATc                                                                                                         <  5:132704/149‑1 (MQ=255)
                                               ggCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTc                                                                                                   <  1:80448/149‑1 (MQ=255)
                                                  gcCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCggg                                                                                                <  3:340098/149‑1 (MQ=255)
                                                      gCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGTCAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGCACCCCTCGCGTACCTTGGCATCCCACTCGGGGTCg                                                                                            >  6:286378/1‑149 (MQ=255)
                                                                ccACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGa                                                                                  <  1:89882/149‑1 (MQ=255)
                                                                ccACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTAGGGGTCGCCTTTTTCGa                                                                                  >  7:257032/1‑149 (MQ=255)
                                                                 cACGGGCTTGGTGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCTTTTCTGCGACAGCTTGGCCAGGGTTCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGAt                                                                                 <  2:112951/149‑1 (MQ=255)
                                                                 cACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGAt                                                                                 >  3:308323/1‑149 (MQ=255)
                                                                 cACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTAAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGAt                                                                                 >  2:200052/1‑149 (MQ=255)
                                                                  aCGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGa                                                                                  >  3:175447/1‑147 (MQ=255)
                                                                  aCGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGa                                                                                  <  4:175447/147‑1 (MQ=255)
                                                                                    gggAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGctct                                                              <  8:76953/149‑1 (MQ=255)
                                                                                      gAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTggg                                                                  <  8:224943/143‑1 (MQ=255)
                                                                                      gAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTggg                                                                  >  7:224943/1‑143 (MQ=255)
                                                                                            gCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCa                                                           <  4:241715/144‑1 (MQ=255)
                                                                                            gCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCa                                                           >  3:241715/1‑144 (MQ=255)
                                                                                             cTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGc                                                     >  2:1949/1‑149 (MQ=255)
                                                                                             cTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGGCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAg                                                      <  8:69254/148‑1 (MQ=255)
                                                                                              tgaTTTGCTGATGTCGGGGCCAATGCATTTCTGCGACAGCTTGGGCAGGGTGGCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATc                                                                                                         <  6:200424/94‑1 (MQ=255)
                                                                                              tCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGCCAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATc                                                                                                         >  5:200424/1‑96 (MQ=255)
                                                                                              tCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGACTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCGTACAGCg                                                    >  3:299726/1‑149 (MQ=255)
                                                                                                aTTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGt                                                  >  3:145866/1‑149 (MQ=255)
                                                                                                aTTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCACACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGt                                                  >  2:272852/1‑149 (MQ=255)
                                                                                                    gCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACATCGGTccgc                                              >  6:276364/1‑149 (MQ=255)
                                                                                                        aTGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTc                                                            <  7:136538/131‑1 (MQ=255)
                                                                                                        aTGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTc                                                            >  8:136538/1‑131 (MQ=255)
                                                                                                        aTGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGc                                          >  7:241994/1‑149 (MQ=255)
                                                                                                          gTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGcc                                         <  8:257032/148‑1 (MQ=255)
                                                                                                           tCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAg                                       >  3:98573/1‑149 (MQ=255)
                                                                                                             ggCGACAATCCATTTCTGCGACTGCTTGGCCAGGGTGCCATGAGCCTTCAGCTCGGCGAACACCTGGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTGGGGCGCTTCATACAGCGGTGCGCCGGCCAGCt                                     <  5:276364/149‑1 (MQ=255)
                                                                                                                 ccAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTg                                   >  2:290946/1‑147 (MQ=255)
                                                                                                                  cAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTc                                                            <  6:230694/121‑1 (MQ=255)
                                                                                                                  cAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTc                                                            >  5:230694/1‑121 (MQ=255)
                                                                                                                   aaTCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAgc                               >  7:342186/1‑149 (MQ=255)
                                                                                                                     tCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCGACCGCGTTGGGc                                                                 <  2:128546/113‑1 (MQ=255)
                                                                                                                     tCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAgcgc                             >  2:84560/1‑149 (MQ=255)
                                                                                                                       cATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAGCGCtt                           <  7:192298/149‑1 (MQ=255)
                                                                                                                       cATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAGCGCtt                           <  1:84560/149‑1 (MQ=255)
                                                                                                                       cATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAGCGCtt                           <  7:187062/149‑1 (MQ=255)
                                                                                                                            cTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGc                                                                              <  3:46165/93‑1 (MQ=255)
                                                                                                                            cTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGc                                                                              >  4:46165/1‑93 (MQ=255)
                                                                                                                             tGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAGCGCTTGTc                        >  6:181746/1‑146 (MQ=255)
                                                                                                                             tGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAGCGCTTGTc                        <  5:181746/146‑1 (MQ=255)
                                                                                                                                   aGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTAGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAGCGCTTGTCATGCTGCAg               >  7:317263/1‑149 (MQ=255)
                                                                                                                                   aGCTTGGCCAGGGTCCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAg               <  4:145866/149‑1 (MQ=255)
                                                                                                                                      ttGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGgcg            >  7:313371/1‑149 (MQ=255)
                                                                                                                                      ttGGCCAGGGTACCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAgcgc                             <  8:139701/132‑1 (MQ=255)
                                                                                                                                      ttGGCCAGGGTACCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAgcgc                             >  7:139701/1‑132 (MQ=255)
                                                                                                                                         gCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCgg         >  2:232524/1‑149 (MQ=255)
                                                                                                                                               gTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTgcgc   >  5:162793/1‑149 (MQ=255)
                                                                                                                                                tGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGcgcg  <  4:308323/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGCGCCAGCCAGGTCAGCAGTAACCGGGTGCGAAAGCCGAACAGGCCGGCGCCCGCAGGCTTTGCCACGGGCTTGGGGGAGTGAGGGAAGGTGCTCATTTGCTGATGTCGGCGCCAATCCATTTCTGCGACAGCTTGGCCAGGGTGCCATCAGCCTTCAGCTCGGCGAACACCTCGCGTACCTTGGCATCCCACTCGGGGTCGCCTTTTTCGATGGCCACCGCGTTGGGCTCTTCATACAGCGGTGCGCCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCG  >  NC_002947/764423‑764715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑