Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 884,562 A→T 7.0% Q158L (CAG→CTG)  PP_0767 → LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947884,5620AT 7.0% 210.6 / 4.9 71Q158L (CAG→CTG) PP_0767LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (37/29);  new base T (3/2);  total (40/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

GCCAGGCCATGGGCCTGCTGAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCTTAACGAACAGGAC  >  NC_002947/884415‑884710
                                                                                                                                                   |                                                                                                                                                    
gccaggccaTGGGCCTGCTGAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAg                                                                                                                                                     >  5:123468/1‑149 (MQ=255)
  caggccaTGGGCCTGCTGAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAggg                                                                                                                                                   >  6:116680/1‑149 (MQ=255)
   aggccaTGGGCCTGCTGAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAgggg                                                                                                                                                  <  8:241275/149‑1 (MQ=255)
    ggccaTGGGCCTGCTGAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGc                                                                                                                                                 <  6:246556/149‑1 (MQ=255)
             ccTGCTGAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTc                                                                                                                                         >  6:116649/1‑148 (MQ=255)
                 ctgAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCg                                                                                                                                    >  4:58413/1‑149 (MQ=255)
                   gAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGtt                                                                                                                                  >  3:66874/1‑149 (MQ=255)
                    aaaaTGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGtt                                                                                                                                  <  2:70115/148‑1 (MQ=255)
                     aaaTGCGCCAGCGCCATCATCTGTTTGCCTTTGCACAGTGGCTCGACGGCCTGCTCGACGAGCGGGCTCTGTACCTGAAAGCTGATACGCGGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGc                                                                                                                                <  4:27903/149‑1 (MQ=255)
                       aTGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCt                                                                                                                              <  2:290929/149‑1 (MQ=255)
                           gccagcgccaTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATAACCTGGCCCAGGGGCCGGTGCTAGACCGTTGCCTGCAg                                                                                                                          >  6:310857/1‑149 (MQ=255)
                              agcggcaTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGttc                                                                                                                       >  3:213400/1‑149 (MQ=255)
                                   caTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGcc                                                                                                                               >  2:154999/1‑136 (MQ=255)
                                   caTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGcc                                                                                                                               <  1:154999/136‑1 (MQ=255)
                                   caTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCt                                                                                                                  <  7:201176/149‑1 (MQ=255)
                                   caTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCt                                                                                                                  <  5:66616/149‑1 (MQ=255)
                                   caTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCt                                                                                                                  >  5:54936/1‑149 (MQ=255)
                                   caTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCt                                                                                                                  <  3:232907/149‑1 (MQ=255)
                                        gCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCACTGCTGCTGGTACTGGACGACTATCACCTGGCCCAgg                                                                                                                                                    >  2:178512/1‑110 (MQ=255)
                                        gCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCACTGCTGCTGGTACTGGACGACTATCACCTGGCCCAgg                                                                                                                                                    <  1:178512/110‑1 (MQ=255)
                                                    tGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCg                                                                                                 >  6:282054/1‑149 (MQ=255)
                                                       agagTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGcc                                                                                              >  1:361270/1‑149 (MQ=255)
                                                        gagTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCt                                                                                             <  2:299571/149‑1 (MQ=255)
                                                        gagTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCt                                                                                             >  7:243317/1‑149 (MQ=255)
                                                         agTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTg                                                                                            <  5:116680/149‑1 (MQ=255)
                                                               tCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTg                                                                                      >  5:373452/1‑149 (MQ=255)
                                                                 gACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCt                                                                                    <  6:297851/149‑1 (MQ=255)
                                                                  aCGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGgt                                                                                          >  2:32363/1‑142 (MQ=255)
                                                                  aCGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGgt                                                                                          <  1:32363/142‑1 (MQ=255)
                                                                  aCGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTg                                                                                   >  7:185335/1‑149 (MQ=255)
                                                                  aCGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTg                                                                                   <  4:324871/149‑1 (MQ=255)
                                                                  aCGGCCTGCTCGACGAGCGGGCGCTGTACCTGCAAGCGGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCGCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTg                                                                                   <  5:310857/149‑1 (MQ=255)
                                                                    ggCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGt                                                                                 <  4:213400/149‑1 (MQ=255)
                                                                        tGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCg                                                                                                 <  6:35000/129‑1 (MQ=255)
                                                                        tGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCATCAATCACCTGCCCCCCg                                                                                                 >  5:35000/1‑129 (MQ=255)
                                                                             gacgaGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAgc                                                                          <  2:248530/147‑1 (MQ=255)
                                                                                      gcgcTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGc                                                                >  7:271648/1‑148 (MQ=255)
                                                                                            tACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCt                                                          <  2:361270/148‑1 (MQ=255)
                                                                                            tACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCt                                                          <  5:282054/148‑1 (MQ=255)
                                                                                              ccTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTgg                                                        <  5:298817/148‑1 (MQ=255)
                                                                                              ccTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTgg                                                        >  1:199196/1‑148 (MQ=255)
                                                                                                tGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCAc                                                     >  2:335331/1‑149 (MQ=255)
                                                                                                tGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAACTGCCgggacaggcg                                                     >  6:378323/1‑142 (MQ=255)
                                                                                                 gCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGc                                                           >  2:244216/1‑142 (MQ=255)
                                                                                                 gCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGc                                                           <  1:244216/142‑1 (MQ=255)
                                                                                                   aaGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTg                                                  >  8:67690/1‑149 (MQ=255)
                                                                                                      cTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGgcg                                               >  5:346530/1‑149 (MQ=255)
                                                                                                             gccgcTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCgcgcctgc                                        <  4:225866/149‑1 (MQ=255)
                                                                                                             gccgcTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCgcgcctgc                                        >  7:265494/1‑149 (MQ=255)
                                                                                                                gctgctgctGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCgcgcctgcgcc                                     <  5:116649/149‑1 (MQ=255)
                                                                                                                       ctgGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCg                              >  2:249185/1‑149 (MQ=255)
                                                                                                                        tgGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCgg                             >  4:117160/1‑149 (MQ=255)
                                                                                                                          gTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCgg                             >  1:110005/1‑147 (MQ=255)
                                                                                                                          gTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCa                           <  3:46213/149‑1 (MQ=255)
                                                                                                                              tGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTg                       >  5:361443/1‑149 (MQ=255)
                                                                                                                                gacgacTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGt                     >  3:72127/1‑149 (MQ=255)
                                                                                                                                gacgacTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGt                     >  7:97954/1‑149 (MQ=255)
                                                                                                                                gacgacTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGt                     >  6:86986/1‑149 (MQ=255)
                                                                                                                                gacgacTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGC‑GTTGGTc                    >  2:253321/1‑149 (MQ=255)
                                                                                                                                    acTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAg                 <  6:361443/149‑1 (MQ=255)
                                                                                                                                      tATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCt               >  5:205695/1‑149 (MQ=255)
                                                                                                                                      gagcACCGGCCCCTGGGCCAGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTg                       <  5:143960/138‑1 (MQ=255)
                                                                                                                                      gagcACCGGCCCCTGGGCCAGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTg                       >  6:149666/4‑141 (MQ=255)
                                                                                                                                      gagcACCGGCCCCTGGGCCAGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTg                       >  6:143960/4‑141 (MQ=255)
                                                                                                                                      gagcACCGGCCCCTGGGCCAGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTg                       <  5:149666/138‑1 (MQ=255)
                                                                                                                                        tCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCCCCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCtt              >  4:54112/1‑148 (MQ=255)
                                                                                                                                        tCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCt               <  4:66874/147‑1 (MQ=255)
                                                                                                                                        gcACCGGCCCCTGGGCCAGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCtt              >  2:22429/2‑148 (MQ=255)
                                                                                                                                           ccTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCTTAAc           >  7:220217/1‑148 (MQ=255)
                                                                                                                                           ccTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCTTAACg          <  8:265494/149‑1 (MQ=255)
                                                                                                                                                 ccAGGGGCCGCTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACAAGCCCCCCGGCCTGGTGTTGCTGGAGACCAGCCGCCAGCGGCCAGGCTGGCACCTGACGCGCCTGCACCTGTCCCGCCAGTTCGTCGACCTTAAAGACCAg     >  4:253896/1‑148 (MQ=255)
                                                                                                                                                   aGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCTTAACGAACAGGAc  <  1:249185/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCCAGGCCATGGGCCTGCTGAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCTTAACGAACAGGAC  >  NC_002947/884415‑884710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑