Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 884,568 C→A 7.5% P160Q (CCG→CAG)  PP_0767 → LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947884,5680CA 7.5% 214.7 / 6.7 67P160Q (CCG→CAG) PP_0767LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (35/27);  new base A (3/2);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.46e-01

CCTGCTGAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCTTAACGAACAGGACCTGCG  >  NC_002947/884428‑884715
                                                                                                                                            |                                                                                                                                                   
ccTGCTGAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTc                                                                                                                                              >  6:116649/1‑148 (MQ=255)
    ctgAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCg                                                                                                                                         >  4:58413/1‑149 (MQ=255)
      gAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGtt                                                                                                                                       >  3:66874/1‑149 (MQ=255)
       aaaaTGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGtt                                                                                                                                       <  2:70115/148‑1 (MQ=255)
        aaaTGCGCCAGCGCCATCATCTGTTTGCCTTTGCACAGTGGCTCGACGGCCTGCTCGACGAGCGGGCTCTGTACCTGAAAGCTGATACGCGGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGc                                                                                                                                     <  4:27903/149‑1 (MQ=255)
          aTGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCt                                                                                                                                   <  2:290929/149‑1 (MQ=255)
              gccagcgccaTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATAACCTGGCCCAGGGGCCGGTGCTAGACCGTTGCCTGCAg                                                                                                                               >  6:310857/1‑149 (MQ=255)
                 agcggcaTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGttc                                                                                                                            >  3:213400/1‑149 (MQ=255)
                      caTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGcc                                                                                                                                    >  2:154999/1‑136 (MQ=255)
                      caTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGcc                                                                                                                                    <  1:154999/136‑1 (MQ=255)
                      caTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCt                                                                                                                       <  7:201176/149‑1 (MQ=255)
                      caTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCt                                                                                                                       <  5:66616/149‑1 (MQ=255)
                      caTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCt                                                                                                                       <  3:232907/149‑1 (MQ=255)
                      caTCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCt                                                                                                                       >  5:54936/1‑149 (MQ=255)
                                       tGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCg                                                                                                      >  6:282054/1‑149 (MQ=255)
                                          agagTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGcc                                                                                                   >  1:361270/1‑149 (MQ=255)
                                           gagTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCt                                                                                                  >  7:243317/1‑149 (MQ=255)
                                           gagTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCt                                                                                                  <  2:299571/149‑1 (MQ=255)
                                            agTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTg                                                                                                 <  5:116680/149‑1 (MQ=255)
                                                  tCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTg                                                                                           >  5:373452/1‑149 (MQ=255)
                                                    gACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCt                                                                                         <  6:297851/149‑1 (MQ=255)
                                                     aCGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGgt                                                                                               >  2:32363/1‑142 (MQ=255)
                                                     aCGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGgt                                                                                               <  1:32363/142‑1 (MQ=255)
                                                     aCGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTg                                                                                        >  7:185335/1‑149 (MQ=255)
                                                     aCGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTg                                                                                        <  4:324871/149‑1 (MQ=255)
                                                     aCGGCCTGCTCGACGAGCGGGCGCTGTACCTGCAAGCGGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCGCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTg                                                                                        <  5:310857/149‑1 (MQ=255)
                                                       ggCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGt                                                                                      <  4:213400/149‑1 (MQ=255)
                                                           tGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCg                                                                                                      <  6:35000/129‑1 (MQ=255)
                                                           tGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCATCAATCACCTGCCCCCCg                                                                                                      >  5:35000/1‑129 (MQ=255)
                                                                gacgaGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAgc                                                                               <  2:248530/147‑1 (MQ=255)
                                                                         gcgcTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGc                                                                     >  7:271648/1‑148 (MQ=255)
                                                                               tACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCt                                                               <  2:361270/148‑1 (MQ=255)
                                                                               tACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCt                                                               <  5:282054/148‑1 (MQ=255)
                                                                                 ccTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTgg                                                             >  1:199196/1‑148 (MQ=255)
                                                                                 ccTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTgg                                                             <  5:298817/148‑1 (MQ=255)
                                                                                   tGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCAc                                                          >  2:335331/1‑149 (MQ=255)
                                                                                   tGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAACTGCCgggacaggcg                                                          >  6:378323/1‑142 (MQ=255)
                                                                                    gCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGc                                                                >  2:244216/1‑142 (MQ=255)
                                                                                    gCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGc                                                                <  1:244216/142‑1 (MQ=255)
                                                                                      aaGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTg                                                       >  8:67690/1‑149 (MQ=255)
                                                                                         cTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGgcg                                                    >  5:346530/1‑149 (MQ=255)
                                                                                                gccgcTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCgcgcctgc                                             <  4:225866/149‑1 (MQ=255)
                                                                                                gccgcTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCgcgcctgc                                             >  7:265494/1‑149 (MQ=255)
                                                                                                   gctgctgctGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCgcgcctgcgcc                                          <  5:116649/149‑1 (MQ=255)
                                                                                                          ctgGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCg                                   >  2:249185/1‑149 (MQ=255)
                                                                                                           tgGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCgg                                  >  4:117160/1‑149 (MQ=255)
                                                                                                             gTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCgg                                  >  1:110005/1‑147 (MQ=255)
                                                                                                             gTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCa                                <  3:46213/149‑1 (MQ=255)
                                                                                                                 tGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTg                            >  5:361443/1‑149 (MQ=255)
                                                                                                                   gacgacTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGt                          >  7:97954/1‑149 (MQ=255)
                                                                                                                   gacgacTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGt                          >  6:86986/1‑149 (MQ=255)
                                                                                                                   gacgacTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGt                          >  3:72127/1‑149 (MQ=255)
                                                                                                                   gacgacTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGC‑GTTGGTc                         >  2:253321/1‑149 (MQ=255)
                                                                                                                       acTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAg                      <  6:361443/149‑1 (MQ=255)
                                                                                                                         tATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCt                    >  5:205695/1‑149 (MQ=255)
                                                                                                                         gagcACCGGCCCCTGGGCCAGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTg                            >  6:149666/4‑141 (MQ=255)
                                                                                                                         gagcACCGGCCCCTGGGCCAGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTg                            >  6:143960/4‑141 (MQ=255)
                                                                                                                         gagcACCGGCCCCTGGGCCAGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTg                            <  5:143960/138‑1 (MQ=255)
                                                                                                                         gagcACCGGCCCCTGGGCCAGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTg                            <  5:149666/138‑1 (MQ=255)
                                                                                                                           tCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCCCCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCtt                   >  4:54112/1‑148 (MQ=255)
                                                                                                                           tCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCt                    <  4:66874/147‑1 (MQ=255)
                                                                                                                           gcACCGGCCCCTGGGCCAGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCtt                   >  2:22429/2‑148 (MQ=255)
                                                                                                                              ccTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCTTAAc                >  7:220217/1‑148 (MQ=255)
                                                                                                                              ccTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCTTAACg               <  8:265494/149‑1 (MQ=255)
                                                                                                                                    ccAGGGGCCGCTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACAAGCCCCCCGGCCTGGTGTTGCTGGAGACCAGCCGCCAGCGGCCAGGCTGGCACCTGACGCGCCTGCACCTGTCCCGCCAGTTCGTCGACCTTAAAGACCAg          >  4:253896/1‑148 (MQ=255)
                                                                                                                                      aGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCTTAACGAACAGGAc       <  1:249185/149‑1 (MQ=255)
                                                                                                                                          gCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCTTAACGAACAGGACCTgc   >  6:82730/1‑149 (MQ=255)
                                                                                                                                           ccGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCTTAACGAACAGGACCTgcg  >  1:116079/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
CCTGCTGAAAATGCGCCAGCGCCATCAGCCGTTTGCCTTTGAAGAGTGGCTCGACGGCCTGCTCGACGAGCTGGCGCTGTACCTGCAAGCTGATACGCCGCTGCTGCTGGTACTGGACGACTATCACCTGGCCCAGGGGCCGGTGCTCGACCGTTGCCTGCAGTTCTTCCTCAATCACCTGCCCCCCGGCCTGGTGTTGCTGGTGACCAGCCGCCAGCGGCCAGGCTGGCACCTGGCGCGCCTGCGCCTGTCCCGGCAGTTGGTCGAGCTTAACGAACAGGACCTGCG  >  NC_002947/884428‑884715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑