Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 922,097 C→T 7.0% V311I (GTA→ATA)  PP_0803 ← protein secretion ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947922,0970CT 7.0% 164.2 / 2.4 57V311I (GTA→ATA) PP_0803protein secretion ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (19/33);  new base T (2/2);  total (21/36)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

GATCGCAAAACGCTTGCCGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCACGATCTTCATCACCTGGCCACCGGGCTGCACCAC  >  NC_002947/921951‑922241
                                                                                                                                                  |                                                                                                                                                
gATCGCAAAACGCTTGCCGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATAcc                                                                                                                                                 >  7:184106/1‑148 (MQ=255)
  tCGCAAAACGCTTGCCGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGag                                                                                                                                              >  5:3069/1‑149 (MQ=255)
  tCGCAAAACGCTTGCCGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGag                                                                                                                                              >  7:244288/1‑149 (MQ=255)
    gCAAAACGCTTGCCGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGagta                                                                                                                                            <  1:353543/149‑1 (MQ=255)
    gCAAAACGCTTGCCGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGagta                                                                                                                                            <  6:33029/149‑1 (MQ=255)
     cAAAACGCTTGCCGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGagtag                                                                                                                                           <  5:279090/149‑1 (MQ=255)
      aaaaCGCTTGCCGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGagtagt                                                                                                                                          >  6:57995/1‑149 (MQ=255)
      aaaaCGCTTGCCGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCAGCCGTATACCGagtagt                                                                                                                                          >  7:222289/1‑149 (MQ=255)
           gCTTGCCGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAg                                                                                                                                     <  5:57995/149‑1 (MQ=255)
           gCTTGCCGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAg                                                                                                                                     <  3:39699/149‑1 (MQ=255)
               gCCGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCa                                                                                                                                  <  5:319214/148‑1 (MQ=255)
                 cGGCCTTGTTTTGCAAGCTGTCCTGTTCTGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTg                                                                                                                               <  6:89563/149‑1 (MQ=255)
                 cGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTg                                                                                                                               <  2:186695/149‑1 (MQ=255)
                    ccTTGTTTTGCAAGCTGTCCTGTTGGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTTCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATc                                                                                                                            <  3:266166/149‑1 (MQ=255)
                    ccTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATc                                                                                                                            >  2:368993/1‑149 (MQ=255)
                     cTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCt                                                                                                                           <  8:244288/149‑1 (MQ=255)
                        gTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTg                                                                                                                         <  2:359656/148‑1 (MQ=255)
                         ttttGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGa                                                                                                                              <  6:28471/142‑1 (MQ=255)
                         ttttGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGa                                                                                                                              >  5:28471/1‑142 (MQ=255)
                            tGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCtt                                                                                                                    >  3:3365/1‑149 (MQ=255)
                              cAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTg                                                                                                                   <  4:198596/148‑1 (MQ=255)
                                         gTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGa                                                                                                                        <  3:268333/132‑1 (MQ=255)
                                         gTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGa                                                                                                                        >  4:268333/1‑132 (MQ=255)
                                            cGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCggg                                                                                                     <  1:368993/148‑1 (MQ=255)
                                             ggTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGt                                                                                                   <  6:215990/149‑1 (MQ=255)
                                                 cGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTTGTCGGGGtgaat                                                                                               <  6:133861/149‑1 (MQ=255)
                                                  ggATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGtgaat                                                                                               >  6:347738/1‑148 (MQ=255)
                                                     tGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAg                                                                                           <  6:23250/149‑1 (MQ=255)
                                                          acGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGGCGTATTCCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGAt                                                                                      <  8:222289/149‑1 (MQ=255)
                                                                     gtagATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGgcg                                                                           >  6:277590/1‑149 (MQ=255)
                                                                         aTTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGTTGAATGAAGGCGATGTCCCGTGGCGCGAt                                                                       <  8:184106/149‑1 (MQ=255)
                                                                               ggTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGt                                                                 >  6:313568/1‑149 (MQ=255)
                                                                                 tttGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTACCGTGGCGCGATGCGGGttt                                                               <  5:347738/149‑1 (MQ=255)
                                                                                     aCCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGa                                                            <  5:277590/148‑1 (MQ=255)
                                                                                     aCCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGAt                                                           <  4:170066/149‑1 (MQ=255)
                                                                                       cTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGAt                                                           >  8:35660/1‑147 (MQ=255)
                                                                                       cTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGAt                                                           >  1:249359/1‑147 (MQ=255)
                                                                                       cTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATca                                                         <  4:3365/149‑1 (MQ=255)
                                                                                       cTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATca                                                         <  2:249359/149‑1 (MQ=255)
                                                                                        tcctCCTGCAGGCTATCCGGCGAGATGCCTAAAACCTTGCCATCCAGCACGCCGTATACCGAGTAGTAATAGGCACTGATCTTGACCTTGGCCGCCAGGTCGCGGt                                                                                                   >  4:367961/4‑106 (MQ=255)
                                                                                        tCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGt                                                                                                   <  3:367961/106‑1 (MQ=255)
                                                                                          gTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATcagc                                                       <  7:35660/148‑1 (MQ=255)
                                                                                                   ggTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGt                                             <  5:313568/149‑1 (MQ=255)
                                                                                                         cGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCg                                       <  2:186989/149‑1 (MQ=255)
                                                                                                             gagaTGCCTACCACCTTGCCATCCAGCCCGCCGTATATCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCAc                                   >  5:160343/1‑149 (MQ=255)
                                                                                                             gagaTGCCTACCACCTTGCCATCCAGCCCGCCGTATATCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCAc                                   >  5:154773/1‑149 (MQ=255)
                                                                                                               gaTGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCACGa                                 >  2:130715/1‑149 (MQ=255)
                                                                                                                  gCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGCCACGATCt                              >  7:141695/1‑149 (MQ=255)
                                                                                                                    cTACCACCTTGCCATCCAGCCCGCCGTATATCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCACGATCTtc                            <  6:154773/149‑1 (MQ=255)
                                                                                                                    cTACCACCTTGCCATCCAGCCCGCCGTATATCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCACGATCTtc                            <  6:160343/149‑1 (MQ=255)
                                                                                                                     tACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCACGATCTtca                           >  4:76972/1‑149 (MQ=255)
                                                                                                                           cTTTCCATCCAGCCCGCAGTATAACGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCACGATCt                              <  4:288677/140‑1 (MQ=255)
                                                                                                                           cTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCACGATCt                              >  3:293733/1‑140 (MQ=255)
                                                                                                                           cTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCACGATCt                              >  3:288677/1‑140 (MQ=255)
                                                                                                                           cTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCACGATCt                              <  4:293733/140‑1 (MQ=255)
                                                                                                                              gCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCACGATCTTCATCACCTgg                   <  1:130715/148‑1 (MQ=255)
                                                                                                                              gCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCACGATCTTCATCACCTg                    <  3:76972/147‑1 (MQ=255)
                                                                                                                                              tataCCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCACGATCTTCATCACCTGGCCACCGGGCTGcaccac  <  6:3069/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GATCGCAAAACGCTTGCCGGCCTTGTTTTGCAAGCTGTCCTGTTCGGTGCGGATGAACACGCGGTAGTAGTAGATTTCCGGTTTGACCTCGTCCTGCAGGGTATCCGGCGAGATGCCTACCACCTTGCCATCCAGCCCGCCGTATACCGAGTAGTCATAGGCACTGATCTTGACCTTGGCCGCCTGGTCGGGGTGAATGAAGGCGATGTCCCGTGGCGCGATGCGGGTTTCGATCAGCAGGCGTTCGTCCATCGGCACGATCTTCATCACCTGGCCACCGGGCTGCACCAC  >  NC_002947/921951‑922241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑