Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 947,371 G→C 10.3% intergenic (‑3/‑153) norR ← / → hmp DNA‑binding transcriptional regulator/flavohemoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947947,3710GC10.3% 100.0 / 4.4 39intergenic (‑3/‑153)norR/hmpDNA‑binding transcriptional regulator/flavohemoprotein
Reads supporting (aligned to +/- strand):  ref base G (20/15);  new base C (2/2);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.23e-01

AGCAGTGCTGCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAG  >  NC_002947/947232‑947515
                                                                                                                                            |                                                                                                                                                
agcagTGCTGCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTAt                                                                                                                                         <  2:346814/149‑1 (MQ=255)
agcagTGCTGCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTAt                                                                                                                                         >  2:309678/1‑149 (MQ=255)
         gcagcaTCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCTGGGTCGGGCAAAACGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGAGTCCTATGGGTCGa                                                                                                                                  >  1:290029/1‑148 (MQ=255)
            gcaTCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCGAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATCGACCCATAGGAGTCCATATGa                                                                                                                             <  1:55726/149‑1 (MQ=255)
                 gCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACtata                                                                                                                        >  2:351113/1‑149 (MQ=255)
                     ggCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATCGACCCATAGGAGTCCATATGAc                                                                                                                            <  5:358266/141‑1 (MQ=255)
                     ggCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATCGACCCATAGGAGTCCATATGAc                                                                                                                            >  6:358266/1‑141 (MQ=255)
                          cagGCCACGCATGGCTTGCAACAGGCGGCGGTAACGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGgggt                                                                                                               >  2:115814/1‑149 (MQ=255)
                           agGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATGGCGGTACTGGTGGGTGAGCAGGGGGAGGAGGTTGGTGAGGAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAgtggtg                                                                                                              <  5:196953/149‑1 (MQ=255)
                                    aTGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGtttt                                                                                                     <  1:162562/149‑1 (MQ=255)
                                       gCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGa                                                                                                   <  1:309678/148‑1 (MQ=255)
                                       gCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGa                                                                                                   >  3:252348/1‑148 (MQ=255)
                                         ttGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACAt                                                                                                >  6:228497/1‑149 (MQ=255)
                                                             gTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGa                                                                            <  1:353971/149‑1 (MQ=255)
                                                                 cT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGa                                                                            <  1:3984/145‑1 (MQ=255)
                                                                 cT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGa                                                                            >  2:3984/1‑145 (MQ=255)
                                                                 cT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGAtttt                                                                        >  1:237275/1‑149 (MQ=255)
                                                                        gggCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGGGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGc                                                                  <  2:290029/149‑1 (MQ=255)
                                                                                              cTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGc                                            >  7:252143/1‑149 (MQ=255)
                                                                                               tGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCt                                           >  3:82683/1‑149 (MQ=255)
                                                                                                 aCCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTg                                          <  5:228497/148‑1 (MQ=255)
                                                                                                   cAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGTGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTaa                                       >  8:153683/1‑149 (MQ=255)
                                                                                                      gggCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTAAtcg                                    >  4:189473/1‑147 (MQ=255)
                                                                                                        gCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTAGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGT‑ATGGCAg                                 <  1:351113/149‑1 (MQ=255)
                                                                                                          aggaggGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTAATGGCAgg                                >  4:54283/1‑149 (MQ=255)
                                                                                                            gaggGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTAATGGCAgggg                              <  4:252348/149‑1 (MQ=255)
                                                                                                                gtggtgAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGt                          >  3:361196/1‑149 (MQ=255)
                                                                                                                       gCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTTCTGTAATGGCAGGGGCAGTCATTTg                    <  8:252143/148‑1 (MQ=255)
                                                                                                                          gTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCt                                           >  5:355398/1‑122 (MQ=255)
                                                                                                                          gTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGc                >  6:168988/1‑149 (MQ=255)
                                                                                                                          gTGGCTGGGTAGTCATATGGACTCCTCTGGGGCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCt                                           <  6:355398/122‑1 (MQ=255)
                                                                                                                            ctgcgtatAGTCATATCGACCCATAGGAGTCCATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCca              >  4:145435/8‑149 (MQ=38)
                                                                                                                               tgtgTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCt                                           <  2:42984/117‑1 (MQ=255)
                                                                                                                               tgtgTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCt                                           >  1:42984/1‑117 (MQ=255)
                                                                                                                                 tgtAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGc         >  2:16478/1‑149 (MQ=255)
                                                                                                                                      tCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAg     >  2:339018/1‑148 (MQ=255)
                                                                                                                                        atatGGACTCCTATGGGTCGATATGACTATACGCAGGTGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAg  <  7:153683/149‑1 (MQ=255)
                                                                                                                                        atatGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCa                            <  4:271672/123‑1 (MQ=255)
                                                                                                                                        atatGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCa                            >  3:271672/1‑123 (MQ=255)
                                                                                                                                            |                                                                                                                                                
AGCAGTGCTGCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTGGCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAG  >  NC_002947/947232‑947515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑