Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 947,375 T→C 10.0% intergenic (‑7/‑149) norR ← / → hmp DNA‑binding transcriptional regulator/flavohemoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947947,3750TC10.0% 104.1 / 3.2 40intergenic (‑7/‑149)norR/hmpDNA‑binding transcriptional regulator/flavohemoprotein
Reads supporting (aligned to +/- strand):  ref base T (20/16);  new base C (2/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

AGCAGTGCTGCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAGTTGT  >  NC_002947/947232‑947519
                                                                                                                                                |                                                                                                                                                 
agcagTGCTGCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTAt                                                                                                                                              <  2:346814/149‑1 (MQ=255)
agcagTGCTGCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTAt                                                                                                                                              >  2:309678/1‑149 (MQ=255)
         gcagcaTCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCTGGGTCGGGCAAAACGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGAGTCCTATGGGTCGa                                                                                                                                       >  1:290029/1‑148 (MQ=255)
            gcaTCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCGAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATCGACCCATAGGAGTCCATATGa                                                                                                                                  <  1:55726/149‑1 (MQ=255)
                 gCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACtata                                                                                                                             >  2:351113/1‑149 (MQ=255)
                     ggCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATCGACCCATAGGAGTCCATATGAc                                                                                                                                 <  5:358266/141‑1 (MQ=255)
                     ggCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATCGACCCATAGGAGTCCATATGAc                                                                                                                                 >  6:358266/1‑141 (MQ=255)
                          cagGCCACGCATGGCTTGCAACAGGCGGCGGTAACGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGgggt                                                                                                                    >  2:115814/1‑149 (MQ=255)
                           agGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATGGCGGTACTGGTGGGTGAGCAGGGGGAGGAGGTTGGTGAGGAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAgtggtg                                                                                                                   <  5:196953/149‑1 (MQ=255)
                                    aTGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGtttt                                                                                                          <  1:162562/149‑1 (MQ=255)
                                       gCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGa                                                                                                        >  3:252348/1‑148 (MQ=255)
                                       gCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGa                                                                                                        <  1:309678/148‑1 (MQ=255)
                                         ttGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACAt                                                                                                     >  6:228497/1‑149 (MQ=255)
                                                             gTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGa                                                                                 <  1:353971/149‑1 (MQ=255)
                                                                 cT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGa                                                                                 <  1:3984/145‑1 (MQ=255)
                                                                 cT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGa                                                                                 >  2:3984/1‑145 (MQ=255)
                                                                 cT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGAtttt                                                                             >  1:237275/1‑149 (MQ=255)
                                                                        gggCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGGGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑Gc                                                                      <  2:290029/149‑1 (MQ=255)
                                                                                              cTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGc                                                >  7:252143/1‑149 (MQ=255)
                                                                                               tGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                               >  3:82683/1‑149 (MQ=255)
                                                                                                 aCCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTg                                              <  5:228497/148‑1 (MQ=255)
                                                                                                   cAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGTGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTaa                                           >  8:153683/1‑149 (MQ=255)
                                                                                                      gggCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAAtcg                                        >  4:189473/1‑147 (MQ=255)
                                                                                                        gCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTAGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGT‑ATGGCAg                                     <  1:351113/149‑1 (MQ=255)
                                                                                                          aggaggGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAgg                                    >  4:54283/1‑149 (MQ=255)
                                                                                                            gaggGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAgggg                                  <  4:252348/149‑1 (MQ=255)
                                                                                                                gtggtgAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGt                              >  3:361196/1‑149 (MQ=255)
                                                                                                                       gCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTTCTGTAATGGCAGGGGCAGTCATTTg                        <  8:252143/148‑1 (MQ=255)
                                                                                                                          gTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                               >  5:355398/1‑122 (MQ=255)
                                                                                                                          gTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGc                    >  6:168988/1‑149 (MQ=255)
                                                                                                                          gTGGCTGGGTAGTCATATGGACTCCTCTGGGGCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                               <  6:355398/122‑1 (MQ=255)
                                                                                                                            ctgcgtatAGTCATATCGACCCATAGGAGTCCATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCca                  >  4:145435/8‑149 (MQ=38)
                                                                                                                               tgtgTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                               <  2:42984/117‑1 (MQ=255)
                                                                                                                               tgtgTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                               >  1:42984/1‑117 (MQ=255)
                                                                                                                                 tgtAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGc             >  2:16478/1‑149 (MQ=255)
                                                                                                                                      tCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAg         >  2:339018/1‑148 (MQ=255)
                                                                                                                                        atatGGACTCCTATGGGTCGATATGACTATACGCAGGTGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAg      <  7:153683/149‑1 (MQ=255)
                                                                                                                                        atatGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCa                                <  4:271672/123‑1 (MQ=255)
                                                                                                                                        atatGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCa                                >  3:271672/1‑123 (MQ=255)
                                                                                                                                              aCTCCTTTGGGTTCTTATTTCTATTCGCAGGGGGTGGTCGTTTTTTCATGTCCTTCGTTAATCCCCTGTTTTTTGTGCCCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAgttgt  <  1:115814/148‑1 (MQ=255)
                                                                                                                                                tCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCcaca                >  4:153529/1‑131 (MQ=255)
                                                                                                                                                tCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCcaca                <  3:153529/131‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
AGCAGTGCTGCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAGTTGT  >  NC_002947/947232‑947519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑