Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	947,380	T→G	9.5%	intergenic (‑12/‑144)	norR ← / → hmp	DNA‑binding transcriptional regulator/flavohemoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	947,380	0	T	G	9.5%	121.6 / 5.5	42	intergenic (‑12/‑144)	norR/hmp	DNA‑binding transcriptional regulator/flavohemoprotein
Reads supporting (aligned to +/- strand):  ref base T (22/16);  new base G (2/2);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

AGCAGTGCTGCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAGTTGTTGCAATG  >  NC_002947/947232‑947526                                                                                                                                                      |                                                                                                                                                    agcagTGCTGCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTAt                                                                                                                                                     <  2:346814/149‑1 (MQ=255) agcagTGCTGCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTAt                                                                                                                                                     >  2:309678/1‑149 (MQ=255)          gcagcaTCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCTGGGTCGGGCAAAACGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGAGTCCTATGGGTCGa                                                                                                                                              >  1:290029/1‑148 (MQ=255)             gcaTCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCGAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATCGACCCATAGGAGTCCATATGa                                                                                                                                         <  1:55726/149‑1 (MQ=255)                  gCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACtata                                                                                                                                    >  2:351113/1‑149 (MQ=255)                      ggCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATCGACCCATAGGAGTCCATATGAc                                                                                                                                        >  6:358266/1‑141 (MQ=255)                      ggCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATCGACCCATAGGAGTCCATATGAc                                                                                                                                        <  5:358266/141‑1 (MQ=255)                           cagGCCACGCATGGCTTGCAACAGGCGGCGGTAACGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGgggt                                                                                                                           >  2:115814/1‑149 (MQ=255)                            agGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATGGCGGTACTGGTGGGTGAGCAGGGGGAGGAGGTTGGTGAGGAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAgtggtg                                                                                                                          <  5:196953/149‑1 (MQ=255)                                     aTGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGtttt                                                                                                                 <  1:162562/149‑1 (MQ=255)                                        gCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGa                                                                                                               >  3:252348/1‑148 (MQ=255)                                        gCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGa                                                                                                               <  1:309678/148‑1 (MQ=255)                                          ttGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACAt                                                                                                            >  6:228497/1‑149 (MQ=255)                                                              gTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGa                                                                                        <  1:353971/149‑1 (MQ=255)                                                                  cT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGa                                                                                        <  1:3984/145‑1 (MQ=255)                                                                  cT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGa                                                                                        >  2:3984/1‑145 (MQ=255)                                                                  cT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGAtttt                                                                                    >  1:237275/1‑149 (MQ=255)                                                                         gggCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGGGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑Gc                                                                             <  2:290029/149‑1 (MQ=255)                                                                                               cTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGc                                                       >  7:252143/1‑149 (MQ=255)                                                                                                tGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                                      >  3:82683/1‑149 (MQ=255)                                                                                                  aCCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTg                                                     <  5:228497/148‑1 (MQ=255)                                                                                                    cAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGTGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTaa                                                  >  8:153683/1‑149 (MQ=255)                                                                                                       gggCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAAtcg                                               >  4:189473/1‑147 (MQ=255)                                                                                                         gCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTAGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGT‑ATGGCAg                                            <  1:351113/149‑1 (MQ=255)                                                                                                           aggaggGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAgg                                           >  4:54283/1‑149 (MQ=255)                                                                                                             gaggGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAgggg                                         <  4:252348/149‑1 (MQ=255)                                                                                                                 gtggtgAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGt                                     >  3:361196/1‑149 (MQ=255)                                                                                                                        gCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTTCTGTAATGGCAGGGGCAGTCATTTg                               <  8:252143/148‑1 (MQ=255)                                                                                                                           gTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                                      >  5:355398/1‑122 (MQ=255)                                                                                                                           gTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGc                           >  6:168988/1‑149 (MQ=255)                                                                                                                           gTGGCTGGGTAGTCATATGGACTCCTCTGGGGCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                                      <  6:355398/122‑1 (MQ=255)                                                                                                                             ctgcgtatAGTCATATCGACCCATAGGAGTCCATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCca                         >  4:145435/8‑149 (MQ=38)                                                                                                                                tgtgTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                                      <  2:42984/117‑1 (MQ=255)                                                                                                                                tgtgTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                                      >  1:42984/1‑117 (MQ=255)                                                                                                                                  tgtAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGc                    >  2:16478/1‑149 (MQ=255)                                                                                                                                       tCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAg                >  2:339018/1‑148 (MQ=255)                                                                                                                                         atatGGACTCCTATGGGTCGATATGACTATACGCAGGTGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAg             <  7:153683/149‑1 (MQ=255)                                                                                                                                         atatGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCa                                       <  4:271672/123‑1 (MQ=255)                                                                                                                                         atatGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCa                                       >  3:271672/1‑123 (MQ=255)                                                                                                                                               aCTCCTTTGGGTTCTTATTTCTATTCGCAGGGGGTGGTCGTTTTTTCATGTCCTTCGTTAATCCCCTGTTTTTTGTGCCCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAgttgt         <  1:115814/148‑1 (MQ=255)                                                                                                                                                 tCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCcaca                       >  4:153529/1‑131 (MQ=255)                                                                                                                                                 tCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCcaca                       <  3:153529/131‑1 (MQ=255)                                                                                                                                                   cTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAGTTGTTGCAAt   >  2:250794/1‑149 (MQ=255)                                                                                                                                                    tATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAGTTGTTGCAATg  >  1:269303/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                    AGCAGTGCTGCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAGTTGTTGCAATG  >  NC_002947/947232‑947526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑