Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 947,382 G→A 9.5% intergenic (‑14/‑142) norR ← / → hmp DNA‑binding transcriptional regulator/flavohemoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947947,3820GA 9.5% 111.8 / 2.7 42intergenic (‑14/‑142)norR/hmpDNA‑binding transcriptional regulator/flavohemoprotein
Reads supporting (aligned to +/- strand):  ref base G (22/16);  new base A (2/2);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-01

GCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAGTTGTTGCAATGCT  >  NC_002947/947241‑947528
                                                                                                                                              |                                                                                                                                                   
gcagcaTCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCTGGGTCGGGCAAAACGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGAGTCCTATGGGTCGa                                                                                                                                                >  1:290029/1‑148 (MQ=255)
   gcaTCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCGAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATCGACCCATAGGAGTCCATATGa                                                                                                                                           <  1:55726/149‑1 (MQ=255)
        gCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACtata                                                                                                                                      >  2:351113/1‑149 (MQ=255)
            ggCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATCGACCCATAGGAGTCCATATGAc                                                                                                                                          <  5:358266/141‑1 (MQ=255)
            ggCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATCGACCCATAGGAGTCCATATGAc                                                                                                                                          >  6:358266/1‑141 (MQ=255)
                 cagGCCACGCATGGCTTGCAACAGGCGGCGGTAACGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGgggt                                                                                                                             >  2:115814/1‑149 (MQ=255)
                  agGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATGGCGGTACTGGTGGGTGAGCAGGGGGAGGAGGTTGGTGAGGAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAgtggtg                                                                                                                            <  5:196953/149‑1 (MQ=255)
                           aTGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGtttt                                                                                                                   <  1:162562/149‑1 (MQ=255)
                              gCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGa                                                                                                                 >  3:252348/1‑148 (MQ=255)
                              gCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGa                                                                                                                 <  1:309678/148‑1 (MQ=255)
                                ttGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACAt                                                                                                              >  6:228497/1‑149 (MQ=255)
                                                    gTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGa                                                                                          <  1:353971/149‑1 (MQ=255)
                                                        cT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGa                                                                                          <  1:3984/145‑1 (MQ=255)
                                                        cT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGa                                                                                          >  2:3984/1‑145 (MQ=255)
                                                        cT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGAtttt                                                                                      >  1:237275/1‑149 (MQ=255)
                                                               gggCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGGGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑Gc                                                                               <  2:290029/149‑1 (MQ=255)
                                                                                     cTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGc                                                         >  7:252143/1‑149 (MQ=255)
                                                                                      tGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                                        >  3:82683/1‑149 (MQ=255)
                                                                                        aCCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTg                                                       <  5:228497/148‑1 (MQ=255)
                                                                                          cAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGTGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTaa                                                    >  8:153683/1‑149 (MQ=255)
                                                                                             gggCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAAtcg                                                 >  4:189473/1‑147 (MQ=255)
                                                                                               gCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTAGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGT‑ATGGCAg                                              <  1:351113/149‑1 (MQ=255)
                                                                                                 aggaggGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAgg                                             >  4:54283/1‑149 (MQ=255)
                                                                                                   gaggGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAgggg                                           <  4:252348/149‑1 (MQ=255)
                                                                                                       gtggtgAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGt                                       >  3:361196/1‑149 (MQ=255)
                                                                                                              gCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTTCTGTAATGGCAGGGGCAGTCATTTg                                 <  8:252143/148‑1 (MQ=255)
                                                                                                                 gTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                                        >  5:355398/1‑122 (MQ=255)
                                                                                                                 gTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGc                             >  6:168988/1‑149 (MQ=255)
                                                                                                                 gTGGCTGGGTAGTCATATGGACTCCTCTGGGGCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                                        <  6:355398/122‑1 (MQ=255)
                                                                                                                   ctgcgtatAGTCATATCGACCCATAGGAGTCCATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCca                           >  4:145435/8‑149 (MQ=38)
                                                                                                                      tgtgTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                                        >  1:42984/1‑117 (MQ=255)
                                                                                                                      tgtgTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCt                                                        <  2:42984/117‑1 (MQ=255)
                                                                                                                        tgtAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGc                      >  2:16478/1‑149 (MQ=255)
                                                                                                                             tCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAg                  >  2:339018/1‑148 (MQ=255)
                                                                                                                               atatGGACTCCTATGGGTCGATATGACTATACGCAGGTGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAg               <  7:153683/149‑1 (MQ=255)
                                                                                                                               atatGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCa                                         >  3:271672/1‑123 (MQ=255)
                                                                                                                               atatGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCa                                         <  4:271672/123‑1 (MQ=255)
                                                                                                                                     aCTCCTTTGGGTTCTTATTTCTATTCGCAGGGGGTGGTCGTTTTTTCATGTCCTTCGTTAATCCCCTGTTTTTTGTGCCCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAgttgt           <  1:115814/148‑1 (MQ=255)
                                                                                                                                       tCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCcaca                         >  4:153529/1‑131 (MQ=255)
                                                                                                                                       tCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCcaca                         <  3:153529/131‑1 (MQ=255)
                                                                                                                                         cTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAGTTGTTGCAAt     >  2:250794/1‑149 (MQ=255)
                                                                                                                                          tATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAGTTGTTGCAATg    >  1:269303/1‑149 (MQ=255)
                                                                                                                                             gggTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAGTTGTTGCAATGCt  >  5:277818/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
GCAGCATCGCACGGCAGCAGGCCACGCATGGCTTGCAACAGGCGGCGGTAGCGTTCCT‑GGTCGGGCAAATCGCGGGACAGGTCGCTGACCAGGGGCAGGAGGGTGGTGAGCAGTGGCTGTGTAGTCATATGGACTCCTATGGGTCGATATGACTATACGCAGGGGTGGGTCGTTTTGACATGACCTGCGTCAAGCCCCTGATTTTGCTG‑GCGATAAAAAGTTGGCACGATTGCTGTAATGGCAGGGGCAGTCATTTGAAGCCACAGGCTCAGGAGTTGTTGCAATGCT  >  NC_002947/947241‑947528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑